FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1236517
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTG64100.5183915789269375No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAATACCAC52500.424579686328615No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC51320.4150367524263718No Hit
GTAATAGTCAGCCTCATCCTCTGACTGGAGGCCAGAGATGAGTAAAATCC50790.4107505194024829No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49320.3988622881852817No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48330.3908559283859421No Hit
GACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAATACCACAGCGTTGTTGTGCCAAATCATACAGTAATAGTCAG43040.34807447046825885No Hit
GTAATAGACAGCCGTGTCCTCGTGTCTCAGGCTGTTCATTTGCAGAAACA42020.3398254937053029No Hit
GTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAG41070.3321426231907851No Hit
GACTATTACTGTATGATTTGGCACAACAACGCTGTGGTATTCGGCGGAGGGACTAAGCTGACCGTCCTAGGTCAG38950.31499769109522957No Hit
GGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCC37950.30691045897468455No Hit
GTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATC35660.2883906974186364No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTAGTCCCTC33160.26817261711727375No Hit
GCTTAGTCCCTCCGCCGAATACCACAGCGTTGTTGTGCCAAATCATACAGTAATAGTCAGCCTCATCCTCTGA32050.2591957894634688No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31710.25644613054248344No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30810.2491676216339929No Hit
GGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGTCTCAC30350.2454474948585422No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG30020.24277870825876233No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT29370.23752200738040805No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA29350.23736026273799712No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28920.23388275292616276No Hit
CACCTGCACCCTACGCAGTGGCATCAATGTTGGTACGTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTC28870.23347839132013554No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG28330.2291112859750412No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT27730.22425894670271415No Hit
GTACGGGACTGTGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA27620.2233693511694542No Hit
CCCCAGTATCTCCTGAGGTTCAAATCAGACTCAGATAAGCAGTGGGGCTC26340.21301769405515655No Hit
CTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAG26240.21220897084310203No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26190.21180460923707478No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26180.21172373691586932No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAATAC25800.20865058871006223No Hit
CAGATAAGCAGTGGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAA24900.20137207980157165No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG24450.19773282534732642No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24100.19490229410513563No Hit
GTCTATTACTGTGCGAGAGGTGGTGTAGTAGACTTTGACTACTGGGGCCA23950.19368920928705388No Hit
GGACTAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG23850.19288048607499939No Hit
CAGTAATAGTCAGCCTCATCCTCTGACTGGAGGCCAGAGATGAGTAAAAT23720.1918291458993285No Hit
ATCCTGTACGTACCAACATTGATGCCACTGCGTAGGGTGCAGGTGAGACT23550.19045431643883587No Hit
GGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCT23430.18948384858437045No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC23310.18851338072990506No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22830.18463150931204342No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22790.18430802002722163No Hit
CCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCT22470.1817201057486472No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22460.18163923342744176No Hit
ATGTTGGTACGTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTGAGGTTCAAAT22410.1812348718214145No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG21970.17767648968837468No Hit
GTATATCCTGTACGTACCAACATTGATGCCACTGCGTAGGGTGCAGGTGA20830.16845704507095333No Hit
GATAAGCAGTGGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTA20700.16740570489528248No Hit
GCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTACTCATCTCTGGC20550.1661926200772007No Hit
CTATTACTGTATGATTTGGCACAACAACGCTGTGGTATTCGGCGGAGGGACTAAGCTGACCGTCCTAGGTCAGCC19660.15899498348991564No Hit
CTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAG19510.15778189867183387No Hit
ACCATATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACTCACTGTTTCTG19330.15632619689013577No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19300.15608357992651942No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18870.15260607011468502No Hit
GCTTATCTGAGTCTGATTTGAACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATATC18480.14945204958767247No Hit
ACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAATACCACAGCGTTGTTGTGCCAAATCATACAGTAATAGTCAGC18460.14929030494526158No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC18420.14896681566043976No Hit
TCTCTGGCCTCCAGTCAGAGGATGAGGCTGACTATTACTGTATGATTTGG18230.1474302415575362No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTAGTC18210.1472684969151253No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18010.1456510504910163No Hit
GTGGTATTCGGCGGAGGGACTAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCA17920.14492319960016725No Hit
AGTATATCCTGTACGTACCAACATTGATGCCACTGCGTAGGGTGCAGGTGAGACTGGCTGATGCTCCAGGAGATG17860.14443796567293454No Hit
GCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGT17840.14427622103052362No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17740.14346749781846913No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17710.1432248808548528No Hit
ATACTGGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATATCCTGTACG17650.1427396469276201No Hit
GCCTCATCCTCTGACTGGAGGCCAGAGATGAGTAAAATCCCTGCATTGGCCGAAGCATCTTTGGATCCAGAGAAG17260.1395856264006075No Hit
GGATGAGGCTGACTATTACTGTATGATTTGGCACAACAACGCTGTGGTATTCGGCGGAGGGACTAAGCTGACCGT17100.1382916692613203No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16970.13724032908564943No Hit
CTCCAGAGCCCCACTGCTTATCTGAGTCTGATTTGAACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTG16650.13465241480707504No Hit
GTATATGGTACTACTAGCAGTAGTAATGAATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTG16480.13327758534658238No Hit
CTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCT16230.1312557773164461No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16120.13036618178318615No Hit
GAATACCACAGCGTTGTTGTGCCAAATCATACAGTAATAGTCAGCCTCAT16080.13004269249836437No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC16080.13004269249836437No Hit
GTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGG15860.12826350143184445No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15750.1273739058985845No Hit
GTATCAACGCAGAGTACGGGACTGTGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTC15700.12696954429255725No Hit
CTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCT15590.1260799487592973No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15580.12599907643809183No Hit
ATGCAGGGATTTTACTCATCTCTGGCCTCCAGTCAGAGGATGAGGCTGAC15410.12462424697759918No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15390.12446250233518828No Hit
CTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCC15110.12219807734143566No Hit
GTATCTCCTGAGGTTCAAATCAGACTCAGATAAGCAGTGGGGCTCTGGAG15090.12203633269902475No Hit
GTCTCACCTGCACCCTACGCAGTGGCATCAATGTTGGTACGTACAGGATA14970.12106586484455935No Hit
CCTGCACCCTACGCAGTGGCATCAATGTTGGTACGTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTC14870.12025714163250487No Hit
CTGCTTATCTGAGTCTGATTTGAACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATAT14590.11799271663875223No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14550.11766922735393043No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT14470.11702224878428684No Hit
CAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCC14460.11694137646308138No Hit
GAGTACGGGACTGTGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA14440.11677963182067047No Hit
CTCCTGGAGCATCAGCCAGTCTCACCTGCACCCTACGCAGTGGCATCAAT14380.11629439789343779No Hit
CTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCT14270.11540480236017783No Hit
GTCCTCCCCAGTATCTCCTGAGGTTCAAATCAGACTCAGATAAGCAGTGGGGCTCTGGAGTCCCCAGCCGCTTCT14240.11516218539656146No Hit
GGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCT14230.11508131307535602No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14090.11394910057847972No Hit
GTGCAGGTGAGACTGGCTGATGCTCCAGGAGATGCAGAGAGGGAAGACGG14090.11394910057847972No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13770.1113611862999053No Hit
GCGTTGTTGTGCCAAATCATACAGTAATAGTCAGCCTCATCCTCTGACTGGAGGCCAGAGATGAGTAAAATCC13670.1105524630878508No Hit
GTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTG13630.11022897380302898No Hit
GGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTT13520.10933937826976903No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG13300.10756018720324911No Hit
GAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTC13010.10521488988829106No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC13000.10513401756708561No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12940.1046487836398529No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC12650.10230348632489483No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT12430.10052429525837495No Hit
CTCCTGAGGTTCAAATCAGACTCAGATAAGCAGTGGGGCTCTGGAGTCCC12430.10052429525837495No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTAT200.007734083651.10362210
GGTATCA10450.043.3869741
TGGTATC3200.040.45542
AATGGTA2700.040.42722770
GTATCAA26850.039.6126861
ATTGTCT10000.034.89001564
CGGGGTA400.002513406834.72928245
TATCAAC30800.034.3995322
ATTTCCG450.002681012734.27477368
TACGGAC400.002761999634.0677032
GCATTGT10200.034.034462
TTGGCAT10750.033.03639259
TTTATTC3100.032.9620824
GGCATTG10550.032.8035561
ATCAACG32650.032.4438443
TGGCATT10750.032.11435760
TCAACGC32900.032.0937774
AACGCAG33150.032.065026
CAACGCA33150.031.9596635
GTGAGTT10750.031.59898851
Produced by FastQC (version 0.11.5)