FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863865
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC30540.353527460887986No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG29150.3374369837879761No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT28970.33535332488293884No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA28440.3292181069958848No Hit
GAGTATTACTGCAGCTCATATGCAGGCAGGAAGACTTTTGTCTTCGGAACTGGGACCACGGTCACCGTCCTAGGT27760.32134650668796627No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC26080.30189902357428533No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC25820.2988892940447871No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25760.29819474107644134No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG25620.2965741174836346No Hit
GTATTACTGCAGCTCATATGCAGGCAGGAAGACTTTTGTCTTCGGAACTG24940.2887025171757161No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC24640.2852297523339874No Hit
GTAATATATAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACA24620.2849982346778721No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCGGCAGCGACGTT23690.274232663668513No Hit
GACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAAGTCTTCCTGCCTGCATATGAGCTGCAGTAATACT20580.23823166814259172No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC20090.23255948556776812No Hit
ACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAAGTCTTCCTGC19670.22769761478934786No Hit
CTCCTGCACTGGAACCGGCAGCGACGTTGGTGGCTATAACTATGTCTCCTGGTACCAACGGCACCCAGGCAAAGC19310.22353029697927337No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19260.22295150283898527No Hit
ATATATTACTGTGCGAGAGGAGAGGAGCGACGGGAGCAACTCCCTGACTA18940.21924722034114127No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC18420.21322776128214477No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17790.2059349551145144No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG17350.20084156667997893No Hit
GCCCTCAGGGGTCCCTAATCGCTTCTTTGGCTCCAAGTCTGGCAACACGG17300.20026277253969083No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG17130.19829487246271119No Hit
GCCTTGGGCTGACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAAGTCTTCCTGCCTGCATATGAGCTG17060.19748456066630782No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG16730.19366451934040618No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT16340.18914992504615882No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAAGTCTTC15550.18000497762960646No Hit
GTCAGTCACCATCTCCTGCACTGGAACCGGCAGCGACGTTGGTGGCTATAACTATGTCTCCTGGTACCAACGGCA15480.1791946658332031No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG13530.15662169436196627No Hit
GCAGTAATACTCAGCCTCATCCTCAGGCTGGAGACCAGAGACGGTCAGGG13520.15650593553390865No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC13510.15639017670585104No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT13270.15361196483246806No Hit
TCTCTGGTCTCCAGCCTGAGGATGAGGCTGAGTATTACTGCAGCTCATAT13150.15222285889577655No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG13150.15222285889577655No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT12790.14805554108570204No Hit
GGCTATAACTATGTCTCCTGGTACCAACGGCACCCAGGCAAAGCCCCCAAACTCATCATTTATGACGTCACTGAG12620.14608764100872243No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA12460.14423549975980043No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC12000.1389105936691497No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT11870.13740572890440056No Hit
GTAATACTCAGCCTCATCCTCAGGCTGGAGACCAGAGACGGTCAGGGAGG11810.13671117593605484No Hit
GGATGAGGCTGAGTATTACTGCAGCTCATATGCAGGCAGGAAGACTTTTGTCTTCGGAACTGGGACCACGGTCAC11510.1332384110943261No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA11500.13312265226626846No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG11440.1324280992979227No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT11390.13184930515763457No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA11260.13034444039288548No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA10940.12664015789504146No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC10900.12617712258281097No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC10660.12339891070942799No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT10650.12328315188137036No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG10320.11946311055546874No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC10220.1183055222748925No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10220.1183055222748925No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC10080.11668489868208576No Hit
CTATAACTATGTCTCCTGGTACCAACGGCACCCAGGCAAAGCCCCCAAAC10050.11633762219791287No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCGGCAGCGACGTTGGTGG9980.1155273104015095No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9880.11436972212093324No Hit
CTCCCTGACCGTCTCTGGTCTCCAGCCTGAGGATGAGGCTGAGTATTACTGCAGCTCATATGCAGGCAGGAAGA9870.11425396329287563No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC9760.11298061618424174No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG9740.1127490985281265No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9670.11193878673172314No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC9560.11066543962308927No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT9530.11031816313891638No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9500.10997088665474351No Hit
CTATGTCTCCTGGTACCAACGGCACCCAGGCAAAGCCCCCAAACTCATCA9210.10661388064107238No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT9180.10626660415689951No Hit
CCTCAGGGGTCCCTAATCGCTTCTTTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGTCTCCAGC9060.10487749822020802No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT9040.10464598056409277No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG8960.10371990993963177No Hit
CTATATGGACTCTCTTGAGGGACGTTTCACCATCTCCAGAGACAACGACAAGATGTCACTGTTTCTGCAAATGAA8930.10337263345545888No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC8900.10302535697128604No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8780.10163625103459452No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC8680.10047866275401827No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG8660.10024714509790304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2400.056.53823570
GGTATCA7800.045.22241
CGTATGC1700.044.7408541
TCGTATG1700.044.68597840
ATGCCGT1750.041.6159344
TTATACA1900.041.4317972
TGGTATC1900.041.4317972
TCTCGTA1850.040.97903438
GTGGTAT2050.040.0929871
ATCTCGT1900.039.87275737
ACGAGAC1900.039.6327122
TCTCCGA1900.039.62812411
TCTGGAG7050.038.49411870
TATACAC2050.038.4002043
GTATCAA19300.037.794911
GTATGCC1950.037.27766842
CATCTCG2050.036.94017836
CTACATC2200.035.9129733
ATCTCCG2100.035.8540210
CCGTCTT2150.035.6138747
Produced by FastQC (version 0.11.5)