FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863865
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC32320.3741325322822432No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA28420.3289865893397695No Hit
GAGTATTACTGCAGCTCATATGCAGGCAGGAAGACTTTTGTCTTCGGAACTGGGACCACGGTCACCGTCCTAGGT28330.3279447598872509No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT26800.3102336591944343No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC25340.29333287029802113No Hit
GTATTACTGCAGCTCATATGCAGGCAGGAAGACTTTTGTCTTCGGAACTG24160.2796733285872214No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG23770.27515873429297405No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC22950.26566651039224876No Hit
GTAATATATAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACA22410.25941553367713704No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC22200.2569845982879269No Hit
GACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAAGTCTTCCTGCCTGCATATGAGCTGCAGTAATACT21330.24691358024691357No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20530.23765287400230362No Hit
ATATATTACTGTGCGAGAGGAGAGGAGCGACGGGAGCAACTCCCTGACTA20300.23499042095697825No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG20230.23418010916057488No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19890.23024430900661563No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCGGCAGCGACGTT18580.21507990253106676No Hit
ACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAAGTCTTCCTGC18320.21207017300156855No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC18150.2101022729245889No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18120.20975499644041604No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG17860.20674526691091777No Hit
GCCTTGGGCTGACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAAGTCTTCCTGCCTGCATATGAGCTG17020.1970215253540773No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG16420.19007599567061984No Hit
GCCCTCAGGGGTCCCTAATCGCTTCTTTGGCTCCAAGTCTGGCAACACGG16270.18833961324975546No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16230.18787657793752496No Hit
CTCCTGCACTGGAACCGGCAGCGACGTTGGTGGCTATAACTATGTCTCCTGGTACCAACGGCACCCAGGCAAAGC16020.18544564254831486No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAAGTCTTC15960.18475108957996908No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT15740.18220439536270136No Hit
GCAGTAATACTCAGCCTCATCCTCAGGCTGGAGACCAGAGACGGTCAGGG14520.16808181833967115No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG14320.16576664177851863No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG13850.1603259768598103No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG13690.15847383561088826No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC13680.15835807678283065No Hit
GTAATACTCAGCCTCATCCTCAGGCTGGAGACCAGAGACGGTCAGGGAGG13170.15245437655189178No Hit
TCTCTGGTCTCCAGCCTGAGGATGAGGCTGAGTATTACTGCAGCTCATAT12380.14330942913533942No Hit
GGCTATAACTATGTCTCCTGGTACCAACGGCACCCAGGCAAAGCCCCCAAACTCATCATTTATGACGTCACTGAG12220.14145728788641743No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA11870.13740572890440056No Hit
GTCAGTCACCATCTCCTGCACTGGAACCGGCAGCGACGTTGGTGGCTATAACTATGTCTCCTGGTACCAACGGCA11620.13451175820295994No Hit
GGATGAGGCTGAGTATTACTGCAGCTCATATGCAGGCAGGAAGACTTTTGTCTTCGGAACTGGGACCACGGTCAC11210.12976564625259734No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA11000.12733471086338724No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA10530.12189404594467886No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC10450.12096797532021786No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT10370.12004190469575686No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC10350.1198103870396416No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC10300.11923159289935349No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT9980.1155273104015095No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG9960.11529579274539424No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9940.115064275089279No Hit
GTTTGGGGGCTTTGCCTGGGTGCCGTTGGTACCAGGAGACATAGTTATAG9870.11425396329287563No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC9760.11298061618424174No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC9750.11286485735618414No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG9680.11205454555978074No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT9590.11101271610726213No Hit
CTCCCTGACCGTCTCTGGTCTCCAGCCTGAGGATGAGGCTGAGTATTACTGCAGCTCATATGCAGGCAGGAAGA9570.11078119845114688No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9560.11066543962308927No Hit
CCTGAGGGCCGCTCAGTGACGTCATAAATGATGAGTTTGGGGGCTTTGCC9450.10939209251445538No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG9240.10696115712524527No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT9160.10603508650078428No Hit
CTATAACTATGTCTCCTGGTACCAACGGCACCCAGGCAAAGCCCCCAAAC9010.10429870407991988No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG8990.10406718642380465No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAAAGAAGCGATTAGGGAC8950.10360415111157414No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC8890.1029095981432284No Hit
CCTCAGGGGTCCCTAATCGCTTCTTTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGTCTCCAGC8880.10279383931517078No Hit
CTATGTCTCCTGGTACCAACGGCACCCAGGCAAAGCCCCCAAACTCATCA8800.10186776869070978No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACG200.00761829751.2976721
GGTATCA7850.043.56491
GTATCAA21500.041.0381361
GTGGTAT2300.038.6591151
TGGTATC2400.035.6006972
ATCAACG24650.035.2124523
TATCAAC24750.035.212332
TCTGGAG6800.034.87678570
TCAACGC24900.034.5844354
CAACGCA24950.034.5171285
AACGCAG25000.034.3133776
ACGCAGA26550.032.56767
AGAGTAC27100.032.1588611
CAGAGTA27600.031.94775610
CGCAGAG27100.031.9066338
GCAGAGT28150.031.080749
TAGGACA553.4435312E-431.0661054
AATCGAG450.00482622230.3968622
ATTACCG450.0048385430.38102315
TTAACGG953.296609E-728.95635835
Produced by FastQC (version 0.11.5)