FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944822
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC35630.3771080690331089No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT35050.37096934660708575No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30720.3251406084955685No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCCTGGTCCCA29820.31561500473104986No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC27860.2948703565327649No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC27630.29243603557072123No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG26700.28259291168071865No Hit
GATTATTACTGCTGCTCATATGCGCGTACTGTCAATTCCCCTAATGTCTTCGGAAGTGGGACCAGGGTCACCGTC26620.2817461913460948No Hit
GTTCCAACACCACCCCGGCCAAGCCCCCAGACTCCTAATTTATGAGGGCT25510.26999794670318855No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC25420.2690453863267367No Hit
CTCCTGCACTGGACCCACCAGTGATATTGGCACTTTTAACCTTGTCTCGTGGTTCCAACACCACCCCGGCCAAGC25380.26862202615942476No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT24480.25909642239490616No Hit
GACCTAGGACGGTGACCCTGGTCCCACTTCCGAAGACATTAGGGGAATTG23550.24925329850490358No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC21870.23147217147780216No Hit
ACCTAGGACGGTGACCCTGGTCCCACTTCCGAAGACATTAGGGGAATTGA21690.22956705072489847No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT20950.22173488762962762No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20890.22109984737865968No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20340.21527864507812053No Hit
GCCTTGGGCTGACCTAGGACGGTGACCCTGGTCCCACTTCCGAAGACATT19890.21051584319586122No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG18490.1956982373399434No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCCTGGTC18120.19178215579230795No Hit
CTTTTAACCTTGTCTCGTGGTTCCAACACCACCCCGGCCAAGCCCCCAGA17990.19040623524854416No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGACCCACCAGTGATATT17730.18765439416101656No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG17670.18701935391004867No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG17470.18490255307348896No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG15850.1677564662973555No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG14550.1539972608597175No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG14450.15293886044143765No Hit
GTGTTGGAACCACGAGACAAGGTTAAAAGTGCCAATATCACTGGTGGGTCCAGTGCAGGAGATGGTGATCGA13780.14584757763896267No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC13780.14584757763896267No Hit
GTCGATCACCATCTCCTGCACTGGACCCACCAGTGATATTGGCACTTTTAACCTTGTCTCGTGGTTCCAACACCA13750.14553005751347872No Hit
GCACTGGACCCACCAGTGATATTGGCACTTTTAACCTTGTCTCGTGGTTC13520.1430957365514351No Hit
GCCCTCAGGTGTTTCCTATCGCTTCTCTGCCTCCAGGTCTGGCAATACGG13390.14171981600767128No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC12980.13738037429272393No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT12870.13621613383261608No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12740.1348402132888523No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA12720.13462853320519633No Hit
GACTAGGGACACGTCCATCAATACAGGTTACATGGAACTGTCTAGTCTGACATCTAACGACACGGCCATTTATTA12260.129759891281109No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12250.12965405123928106No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12130.12838397073734523No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG12120.12827813069551725No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12090.1279606105700333No Hit
CATTAGGGGAATTGACAGTACGCGCATATGAGCAGCAGTAATAATCAGCC12000.12700805019358144No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11950.12647884998444153No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC11540.12213940826949414No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11370.12034012755841841No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA11260.11917588709831058No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11220.11875252693099865No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA11000.11642404601078299No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10920.11557732567615911No Hit
GCTCATATGCGCGTACTGTCAATTCCCCTAATGTCTTCGGAAGTGGGACC10900.11536564559250313No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC10850.11483644538336321No Hit
CTCCTAATTTATGAGGGCTTCAAGCGGCCCTCAGGTGTTTCCTATCGCTT10790.11420140513239531No Hit
GGGTGCGACAGGCCCCTGGACAAGGACTGGAGTGGATGGGCTCGATCAAC10660.11282548458863152No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10650.11271964454680353No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG10590.11208460429583562No Hit
ACCCTAAGGGTGACACAAACTATGCACAGAAGTTTCAGGGCAGGGTCACCCTGACTAGGGACACGTCCATCAATA10550.11166124412852367No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG10480.11092036383572779No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10460.11070868375207181No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10440.11049700366841586No Hit
CCCCTAATGTCTTCGGAAGTGGGACCAGGGTCACCGTCCTAGGTCAGCCC10420.11028532358475988No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10220.1081685227482002No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC10210.1080626827063722No Hit
CCTGAGGGCCGCTTGAAGCCCTCATAAATTAGGAGTCTGGGGGCTTGGCC9870.10446412128422074No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC9860.10435828124239276No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9820.1039349210750808No Hit
GTCTGGGGGTGAGATGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCATGCAAGGCTTCTGGATACACCTTCATTGG9800.10372324099142485No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9520.10075971982024126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4100.072.1948170
CGAAATC200.006428006653.54979360
CCCACGA4100.052.34739319
CGTATGC4150.050.88008541
TCGTATG4100.050.56927540
TATACAC4550.049.4111443
GAGCCTA4300.049.27946531
ATCTCGT4050.049.2439137
TATCTCG4050.049.2018636
GTATGCC4300.049.18454442
TCTCGTA4100.048.71624838
AGCCTAT4350.047.97064232
CTTATAC4500.047.7069131
ATGCCGT4450.047.6730144
GCCTATC4250.047.54007733
TCTCCGA4550.047.16518411
TTATACA4700.047.1094322
ATCTCCG4700.045.65991210
TACACAT4900.045.18665
TGCTTGA4800.044.93739755
Produced by FastQC (version 0.11.5)