FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944822
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40810.4319332107000049No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC37300.3947833560183823No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT31270.3309618107961076No Hit
CTCCTGCACTGGACCCACCAGTGATATTGGCACTTTTAACCTTGTCTCGTGGTTCCAACACCACCCCGGCCAAGC26700.28259291168071865No Hit
GTCCTGGGCCCAGTCTGCCCTGACGCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG26470.28015859071867505No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC26330.27867683013308325No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC24650.26089570310598187No Hit
GATTATTACTGCTGCTCATATGCGCGTACTGTCAATTCCCCTAATGTCTTCGGAAGTGGGACCAGGGTCACCGTC24560.25994314272953No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC24380.2580380219766263No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCCTGGTCCCA24350.2577205018511423No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24100.2550745008054427No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG22220.2351765729417816No Hit
GTTCCAACACCACCCCGGCCAAGCCCCCAGACTCCTAATTTATGAGGGCT21770.23041377105952232No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGACCCACCAGTGATATT20730.21940640670941192No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT20340.21527864507812053No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19830.2098808029448933No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACGCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC19440.20575304131360195No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG19150.20268368010059037No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC19110.20226031993327842No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18540.19622743754908334No Hit
CTTTTAACCTTGTCTCGTGGTTCCAACACCACCCCGGCCAAGCCCCCAGA17830.1887127945792964No Hit
GACCTAGGACGGTGACCCTGGTCCCACTTCCGAAGACATTAGGGGAATTG16800.177811270271014No Hit
GCCTTGGGCTGACCTAGGACGGTGACCCTGGTCCCACTTCCGAAGACATT15960.1689207067574633No Hit
GTCGATCACCATCTCCTGCACTGGACCCACCAGTGATATTGGCACTTTTAACCTTGTCTCGTGGTTCCAACACCA15390.16288782437326818No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCCTGGTC15160.16045350341122455No Hit
ACCTAGGACGGTGACCCTGGTCCCACTTCCGAAGACATTAGGGGAATTGA15070.1595009430347727No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG14900.15770166232369695No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG14740.1560082216544492No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG14530.15378558077606153No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA13750.14553005751347872No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG13450.1423548562586392No Hit
GCACTGGACCCACCAGTGATATTGGCACTTTTAACCTTGTCTCGTGGTTC13380.1416139759658433No Hit
GCCCTCAGGTGTTTCCTATCGCTTCTCTGCCTCCAGGTCTGGCAATACGG13340.14119061579853137No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACGCAGC13300.14076725563121942No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC12500.13230005228498065No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC12320.13039493153207696No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT12300.13018325144842097No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC12040.1274314103608934No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12000.12700805019358144No Hit
GGGTGCGACAGGCCCCTGGACAAGGACTGGAGTGGATGGGCTCGATCAAC11960.12658469002626951No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA11950.12647884998444153No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACGCAGCCTGCCTCCGTGTCTGGGTCTCCTGGA11710.12393868898056988No Hit
GACTAGGGACACGTCCATCAATACAGGTTACATGGAACTGTCTAGTCTGACATCTAACGACACGGCCATTTATTA11470.12139852797669824No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG11450.1211868478930423No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11370.12034012755841841No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC11340.12002260743293446No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG11300.11959924726562253No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC11300.11959924726562253No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11280.11938756718196655No Hit
CTCCTAATTTATGAGGGCTTCAAGCGGCCCTCAGGTGTTTCCTATCGCTT11250.11907004705648258No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11200.11854084684734267No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACG10980.11621236592712701No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10630.11250796446314755No Hit
GCTCATATGCGCGTACTGTCAATTCCCCTAATGTCTTCGGAAGTGGGACC10580.11197876425400764No Hit
GTCTGGGGGTGAGATGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCATGCAAGGCTTCTGGATACACCTTCATTGG10520.11134372400303974No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACGCAGCCTGCCT10490.11102620387755577No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10400.11007364350110391No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10100.10689844224626437No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG9980.10562836174432856No Hit
GGACCCACCAGTGATATTGGCACTTTTAACCTTGTCTCGTGGTTCCAACACCACCCCGGCCAAGCCCCCAGACTC9720.10287652065680097No Hit
ACCCTAAGGGTGACACAAACTATGCACAGAAGTTTCAGGGCAGGGTCACCCTGACTAGGGACACGTCCATCAATA9650.10213564036400508No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC9630.10192396028034911No Hit
CCCCTAATGTCTTCGGAAGTGGGACCAGGGTCACCGTCCTAGGTCAGCCC9630.10192396028034911No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9620.10181812023852112No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG9500.10054803973658531No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGACCCACCAGTGATATTGGCA9470.10023051961110135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9750.047.7999271
GTGGTAT2350.041.97991
TGGTATC2400.041.074862
GTATCAA25600.041.0610241
TATCAAC29600.035.0265582
GGATATT4350.034.4092061
ATCAACG30300.033.9893883
TCAACGC30300.033.7650384
AACGCAG30500.033.6550676
CAACGCA30550.033.5999875
AATGGTA2950.032.2747870
ACGCAGA32500.031.6885687
CGCAGAG32400.031.6814678
AGAGTAC33100.031.21683911
TTCTAAC450.004275531.1593149
GCAGAGT33850.030.4247729
CAGAGTA33750.030.4142110
GACAGGT1251.4733814E-1029.9360081
GATATTT5000.029.2339692
GTGGCCG8650.028.30372870
Produced by FastQC (version 0.11.5)