FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686081
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCACCAGTATGGTAGCTCACCTTGGACGTTCGGCCAAGGGA30950.4511129152388712No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23910.34850112450279197No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17420.25390587991796887No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16920.24661811068955414No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16570.24151667222966386No Hit
GTCTCTGGTGGCTCCATCAACACTTACTATTGGAGCTGGATCCGGCAGCC16310.23772703223088817No Hit
GTAATAAATGGCCGTGTCTGCGGCGGTCACAGAATTCAACTTCAGGGAGA16080.23437465838581745No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA14470.21090804147032202No Hit
GTGTATTACTGTCACCAGTATGGTAGCTCACCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAA14090.20536933685672684No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14060.20493207070302194No Hit
GTATGGTAGCTCACCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT13440.19589523685978769No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAACACTTACTATTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGA13440.19589523685978769No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12650.18438056147889245No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT12120.17665552609677282No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC11690.17038804456033618No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTCTCGGCAACTACTTAACCTGGTCCC11540.16820171379181176No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11490.1674729368689703No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11470.1671814260998337No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11440.16674415994612882No Hit
CATCTATGGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA11440.16674415994612882No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA11100.1617884768708068No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10870.15843610302573602No Hit
ATTTATTACTGTGCGAGACATTATCAGCCGTGGCTGGTACTAGGTTACTT10530.15348041995041403No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10280.14983653533620667No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC10270.14969077995163838No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTCTCGG10250.1493992691825018No Hit
CTACTTAACCTGGTCCCAGCAGAAACCTGGCCAGCCTCCCAGGCTCCTCATCTATGGTGCATCCAACAGGGCCA10240.1492535137979335No Hit
ACCTAGTACCAGCCACGGCTGATAATGTCTCGCACAGTAATAAATGGCCGTGTCTGCGGCGGTCACAGAATTCAA9870.14386056456890658No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9830.1432775430306334No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9630.1403624353392675No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9610.14007092457013093No Hit
GTACTAGGTTACTTTGACCACTGGGGCCAGGGAACCCTGGTCACCGTCTC9250.13482373072567233No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT9140.1332204214954211No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA9030.13161711226516987No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8970.1307425799577601No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8930.13015955841948693No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTC8910.12986804765035032No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8540.12447509842132343No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8490.12374632149848196No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8480.12360056611391365No Hit
CTACCATACTGGTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC8400.1224345230373673No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8100.11806186150031847No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7970.11616704150093066No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC7950.11587553073179406No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7950.11587553073179406No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7820.11398071073240622No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCACCAGTATGGTAGCTCA7790.11354344457870134No Hit
CTACAACCCCTCCCTCAAGAGCCGGGTCACCATATCAGTAGACACGTCCA7710.11237740150215499No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7610.11091984765647205No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7570.11033682611819888No Hit
CCATAGATGAGGAGCCTGGGAGGCTGGCCAGGTTTCTGCTGGGACCAGGTTAAGTAGTTGCCGAGAACACTCTGA7560.11019107073363059No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7490.10917078304165252No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC7410.10800473996510616No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7180.1046523661200354No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7130.10392358919719391No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGGTCAAAGTAACCTAGTA7080.10319481227435245No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7060.10290330150521584No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC6930.10100848150582803No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAG250.00716455152.0979369
TACGTAT400.00791288850.80116370
GGTATCA5600.043.7058451
GTACGTA408.38775E-443.41494469
GTATCAA15950.039.4281231
AATGGTA1905.456968E-1239.2149370
CGGTGTC400.002420716734.9940749
AGGGTAC400.002802843733.9637074
TTATAAC400.002802843733.9637072
AACTGTG13400.033.86744370
TATCAAC18400.033.5945322
ATCAACG18850.032.7925453
AACGCAG18900.032.7081766
CAACGCA19100.032.3633235
TCAACGC19150.032.278824
TACTGAT6800.031.87523770
CGCAGAG19400.031.865188
GTGTAGA652.5669864E-531.3785571
ACGCAGA19850.031.1427977
AGAGTAC20250.030.5276311
Produced by FastQC (version 0.11.5)