FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences963583
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTACCTCACCTTGGACGTTCGGCCAAGGGA50320.5222175982764328No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37770.39197453670311744No Hit
GTATGGTACCTCACCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAGGAACTGTGGCTGCACCAT36290.37661519557733997No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34450.3575197985020491No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA30240.3138286997591282No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAACTCAAGTTCAGGGAGA29420.305318794540792No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA28840.2992995932887982No Hit
GTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC28690.2977429033098342No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23660.24554189934857715No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22190.23028633755473063No Hit
GTGTATTACTGTCAGCAGTATGGTACCTCACCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAGGAA22180.2301825582227997No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC20740.215238334424746No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19860.20610575321482424No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19570.20309615258882735No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19520.2025772559291727No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18990.19707695133683345No Hit
GTCTCTGGTGGCTCCATCAGTACTTACTACTGGAGCTGGATCCGGCAGCC18780.19489758536628396No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18390.19085019142097776No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18310.19001995676553032No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17840.1851423281647767No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16830.17466061563975288No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG16530.17154723568182503No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA16430.17050944236251575No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT16430.17050944236251575No Hit
GTCTATTACTGTGCGAGACATTATCAACAGTGGCTGGTATTAGGTTACTT16280.1689527523835518No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16110.16718850374072602No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCA16000.1660469310894858No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT15590.16179197848031773No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15430.16013150916942287No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14500.15048003129984652No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT13850.14373437472433614No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13780.14300791940081967No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13710.14228146407730316No Hit
CTCCCAGACTCCTCATCTCTGGTGCATCCAGCAGGGCCACTGGCATCCCA13690.14207390541344128No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13670.14186634674957943No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATTTCCACCTTGGTCC13620.1413474500899248No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCACCTACTTAGCCTGGTACC13550.1406209947664083No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13330.13833784946392785No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC13120.13615848349337836No Hit
GCACAAACCTGGCCAGGCTCCCAGACTCCTCATCTCTGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTT12920.1340828968547598No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12870.13356400019510514No Hit
GTGTTAGCAGCACCTACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGACTCCTCATCTCTGGTGCAT12860.13346022086317422No Hit
GTCAGAGTGTTAGCAGCACCTACTTAGCCTGGTACCAGCACAAACCTGGC12790.1327337655396577No Hit
GTATTAGGTTACTTTCACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC12490.12962038558172986No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12440.1291014889220752No Hit
GTGCAGCCACAGTTCCTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC12360.12827125426662778No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12210.12671456428766387No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTACCTCA12180.12640322629187106No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12060.12515787430869993No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12010.12463897764904527No Hit
GAGTGTTAGCAGCACCTACTTAGCCTGGTACCAGCACAAACCTGGCCAGG11920.12370496366166692No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11800.12245961167849578No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC11790.12235583234656486No Hit
TCTCTGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT11700.1214218183591865No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11650.12090292169953186No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA11580.12017646637601535No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11540.11976134904829164No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11500.11934623172056792No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT11400.11830843840125863No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11290.11716686575001842No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11220.11644041042650191No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTACTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGA10990.11405348579209057No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10910.11322325113664314No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10860.11270435447698848No Hit
GGTTTGTGCTGGTACCAGGCTAAGTAGGTGCTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC10830.1123930164811957No Hit
CAGTAATAGACGGCCGTGTCTGCGGCGGTCACAGAACTCAAGTTCAGGGAGAACTGGTTCTTGGACGTGTCTA10810.11218545781733384No Hit
GTCTGGGAGCCTGGCCAGGTTTGTGCTGGTACCAGGCTAAGTAGGTGCTG10810.11218545781733384No Hit
GTACCTCACCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAGGA10680.11083632650223177No Hit
ACCTAATACCAGCCACTGTTGATAATGTCTCGCACAGTAATAGACGGCCG10680.11083632650223177No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10670.11073254717030084No Hit
CCTCATCTCTGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT10500.10896829852747507No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10380.10772294654430392No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10290.10678893255692556No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10250.10637381522920185No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC10230.10616625656533998No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9860.10232642128389562No Hit
GGGTATGTCTTTGACAGTGGGAGCACCAGGTACAACCCCTCCCTCAAGAG9670.10035461397720799No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9650.10014705531334613No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)