FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences963583
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTACCTCACCTTGGACGTTCGGCCAAGGGA46010.4774887062142026No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37560.3897951707325679No Hit
GTATGGTACCTCACCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAGGAACTGTGGCTGCACCAT33210.344651161342614No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA29790.3091586298222364No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27760.2880914254402579No Hit
GTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC27610.28653473546129393No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA27530.28570450080584653No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAACTCAAGTTCAGGGAGA26880.2789588442303361No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23850.24751370665526476No Hit
GTGTATTACTGTCAGCAGTATGGTACCTCACCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAGGAA22280.23122035154210901No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22250.2309090135463162No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21940.2276918542564574No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC20100.20859645718116654No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19370.20102056595020876No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18970.1968693926729716No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18110.18794437012691173No Hit
GTCTCTGGTGGCTCCATCAGTACTTACTACTGGAGCTGGATCCGGCAGCC17630.18296296219422717No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17270.17922690624471374No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCA16670.17300014632885802No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT16630.1725850290011343No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16460.17082078035830853No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16380.1699905457028611No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16250.168641414387759No Hit
GTCTATTACTGTGCGAGACATTATCAACAGTGGCTGGTATTAGGTTACTT15890.1649053584382456No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15480.16065040582907752No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15470.16054662649714657No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA15020.15587655656025481No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14970.15535765990060016No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT14940.15504632190480738No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG14730.15286695593425786No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14660.15214050061074136No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14370.14913089998474444No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13980.1450835060394382No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13590.141036112094132No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT13480.1398945394428918No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC13270.13771517347234227No Hit
CTCCCAGACTCCTCATCTCTGGTGCATCCAGCAGGGCCACTGGCATCCCA13260.13761139414041135No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATTTCCACCTTGGTCC12670.13148841355648658No Hit
GTGTTAGCAGCACCTACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGACTCCTCATCTCTGGTGCAT12630.13107329622876285No Hit
GCACAAACCTGGCCAGGCTCCCAGACTCCTCATCTCTGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTT12610.130865737564901No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12430.12899770959014428No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA12260.1272334609473185No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCACCTACTTAGCCTGGTACC12190.126507005623802No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTACCTCA12040.12495031564483806No Hit
TCTCTGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT12000.12453519831711435No Hit
GTGCAGCCACAGTTCCTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC11900.12349740499780505No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11860.12308228767008136No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11530.11965756971636071No Hit
GTATTAGGTTACTTTCACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC11520.11955379038442977No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11440.11872355572898234No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11400.11830843840125863No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC11320.1174782037458112No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11310.11737442441388027No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC11260.11685552775422563No Hit
GGTTTGTGCTGGTACCAGGCTAAGTAGGTGCTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC11200.11623285176264005No Hit
GTCAGAGTGTTAGCAGCACCTACTTAGCCTGGTACCAGCACAAACCTGGC11110.11529883777526172No Hit
GAGTGTTAGCAGCACCTACTTAGCCTGGTACCAGCACAAACCTGGCCAGG10830.1123930164811957No Hit
CCTCATCTCTGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT10740.11145900249381735No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10700.11104388516609362No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10670.11073254717030084No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10640.11042120917450805No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10500.10896829852747507No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10370.107619167212373No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10250.10637381522920185No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10080.10460956658637606No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10080.10460956658637606No Hit
ACCTAATACCAGCCACTGTTGATAATGTCTCGCACAGTAATAGACGGCCG10070.10450578725444512No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10030.1040906699267214No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9830.10201508328810285No Hit
GTCTGGGAGCCTGGCCAGGTTTGTGCTGGTACCAGGCTAAGTAGGTGCTG9760.10128862796458635No Hit
CAGTAATAGACGGCCGTGTCTGCGGCGGTCACAGAACTCAAGTTCAGGGAGAACTGGTTCTTGGACGTGTCTA9660.10025083464527706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)