FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718958
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38840.5402262719101811No Hit
GAGTTACAGTGCCCCTCCGACATTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT35430.4927965194072533No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33090.4602494165166811No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24520.34104912943454274No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24050.33451189081976973No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23200.322689225239861No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGATGAGAGTGAAATCTGTC22980.3196292412074141No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21770.3027993290289558No Hit
GTGCGGTCCCATCCAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT21550.29973934499650884No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20670.287499408866721No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGCGGTCCCATCCAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA20400.2837439739178088No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACAACATTAACAACTATTTAAATTGGTATCAGCAGAAA19730.2744249316371749No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19270.2680267832056949No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18260.2539786746930975No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17670.24577235387880794No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17590.2446596324124636No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16700.23228060609938272No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG16300.22671699876766097No Hit
GTCTCCGCCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACAACATTAACAA16200.22532609693473055No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16080.22365701473521402No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15370.21378161172140794No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAATGTC15340.2133643411715288No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14540.20223712650808534No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14290.19875987192575925No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14220.1977862406427079No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAATGTCGGAGGGGCA13810.19208354312769313No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT13740.19110991184464182No Hit
GTCCCATCCAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCAT13680.19027537074488357No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGGCCCAGTC13360.18582448487950617No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13310.18512903396304095No Hit
ACTTACTACTGTCAACAGAGTTACAGTGCCCCTCCGACATTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA13170.18318177139693836No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13000.1808172382809566No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12680.17636635241557921No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12400.17247182728337399No Hit
GCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT11640.16190097335310266No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGATGAGAGTGAAATCTGTCCCAGATCCACTGCCA11600.1613446126199305No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11130.15480737400515746No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACA10950.1523037507058827No Hit
GATTTCACTCTCATCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTGCC10690.14868740594026353No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10540.1466010531908679No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10530.14646196300757483No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10350.14395833970830008No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGGCCCAGTCTCCGCCCTCC10310.14340197897512788No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT10230.14228925750878355No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10190.14173289677561138No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9980.13881200292645746No Hit
GGACCGCACTTTGCAAAGTGGATGCAGCATAGATCAGGAGGTTAGGGGCT9960.13853382255987137No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9650.13422202687778703No Hit
ATGCTGCATCCACTTTGCAAAGTGCGGTCCCATCCAGGTTCAGTGGCAGT9630.13394384651120092No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9530.1325529446782705No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT9450.13144022321192614No Hit
CATCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTGCCCCTCCGACATT9200.12796296862960005No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9160.12740660789642788No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9110.12671115697996269No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9040.12573752569691135No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9010.1253202551470322No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCG8840.12295572203105049No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGTTGTTA8840.12295572203105049No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8770.12198209074799919No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8740.12156482019812007No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTGCCCCTCCGACATTCGGCCAAGGGA8700.12100845946494788No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT8690.12086936928165483No Hit
GGATGGGACCGCACTTTGCAAAGTGGATGCAGCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATA8660.1204520987317757No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8630.12003482818189656No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC8630.12003482818189656No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8620.11989573799860354No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8600.11961755763201744No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8510.11836574598238006No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8480.11794847543250093No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8420.11711393433274267No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTGGATGGGACCGCA8300.11544485213322614No Hit
GTGCCAGATGTGACATCCAGATGGCCCAGTCTCCGCCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCA8290.11530576194993311No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8280.11516667176664004No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGG8230.11447122085017485No Hit
CATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTGCCCCTCCGACATTCGG8090.11252395828407222No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8090.11252395828407222No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8040.11182850736760701No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8030.11168941718431395No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC7950.11057669571796962No Hit
GCCCCTAACCTCCTGATCTATGCTGCATCCACTTTGCAAAGTGCGGTCCCATCCAGGTTCAGTGGCAGTGGAT7880.10960306443491832No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7870.10946397425162525No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7670.1066821705857644No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC7660.10654308040247136No Hit
ATCTGGGACAGATTTCACTCTCATCATCAGCAGTCTGCAACCTGAAGATT7660.10654308040247136No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT7530.10473490801966179No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA7530.10473490801966179No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC7510.1044567276530757No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7510.1044567276530757No Hit
CCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGT7500.10431763746978266No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7450.10362218655331744No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7420.1032049160034383No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT7390.10278764545355917No Hit
GCCCAGTCTCCGCCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCAT7270.10111856325404267No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC7210.1002840221542844No Hit
CCCCTAACCTCCTGATCTATGCTGCATCCACTTTGCAAAGTGCGGTCCCA7190.10000584178769831No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTATC200.007287375651.8761742
CGTTTAA200.00758015951.36193531
GGCCGTC200.007693764351.169057
TCGTATG1900.041.76779640
CGTATGC1950.040.74268341
ATGCCGT2100.039.6196844
GTGGTAT2500.039.606541
CGGACTC2000.039.31162328
GACGGAC2000.039.2760426
ATCTCCG2000.039.22960710
GTATGCC2050.038.8016942
GCCGTCT2100.038.09000446
CGAGACG2100.037.38492623
CACGAGA2100.037.38232821
TCTCCGA2100.037.3615311
CCGTCTT2150.037.2490947
GACAGGT1100.037.247531
CTTATAC2200.037.247531
CTTGAAA2200.036.97204657
TATCTCG2050.036.88321336
Produced by FastQC (version 0.11.5)