FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718958
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37430.520614556065862No Hit
GAGTTACAGTGCCCCTCCGACATTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT32250.4485658411200654No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26620.3702580679260819No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23200.322689225239861No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGATGAGAGTGAAATCTGTC22670.3153174455253297No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22370.3111447400265384No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21400.29765299224711317No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20760.28875122051635843No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGCGGTCCCATCCAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA20460.2845785150175671No Hit
GTGCGGTCCCATCCAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT19810.2755376531035193No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19230.26747042247252273No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACAACATTAACAACTATTTAAATTGGTATCAGCAGAAA19230.26747042247252273No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18960.26371498752361056No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18580.2584295605584749No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16040.22310065400204185No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16020.22282247363545576No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15900.22115339143593923No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15560.21642432520397578No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15250.2121125295218914No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15240.21197343933859839No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAATGTC15080.20974799640590966No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14910.2073834632899279No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14740.2050189301739462No Hit
GTCTCCGCCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACAACATTAACAA14340.19945532284222442No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14300.19889896210905228No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13770.19152718239452096No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGGCCCAGTC13590.18902355909524618No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13320.18526812414633398No Hit
GTCCCATCCAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCAT13160.18304268121364528No Hit
ACTTACTACTGTCAACAGAGTTACAGTGCCCCTCCGACATTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA12980.18053905791437053No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12820.17831361498168183No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12750.17733998369863052No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT12700.1766445327821653No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12400.17247182728337399No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAATGTCGGAGGGGCA12120.16857730215116878No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11440.15911916968724182No Hit
GCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT11140.1549464641884505No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGATGAGAGTGAAATCTGTCCCAGATCCACTGCCA10780.14993921758990092No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10730.14924376667343572No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10700.14882649612355658No Hit
GATTTCACTCTCATCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTGCC10560.14687923355745397No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCACA10490.14590560227440266No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGGCCCAGTCTCCGCCCTCC9470.13171840357851225No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9460.1315793133952192No Hit
ATGCTGCATCCACTTTGCAAAGTGCGGTCCCATCCAGGTTCAGTGGCAGT9410.13088386247875397No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9390.1306056821121679No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9370.1303275017455818No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9080.12629388643008355No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT9040.12573752569691135No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8990.12504207478044616No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8950.12448571404727399No Hit
CATCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTGCCCCTCCGACATT8940.12434662386398092No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8860.12323390239763658No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTGGATGGGACCGCA8790.12226027111458526No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8790.12226027111458526No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8690.12086936928165483No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCG8530.11864392634896613No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8460.11767029506591484No Hit
GGACCGCACTTTGCAAAGTGGATGCAGCATAGATCAGGAGGTTAGGGGCT8440.11739211469932874No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8340.1160012128663983No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8330.11586212268310528No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8300.11544485213322614No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGG8200.1140539503002957No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8180.11377576993370961No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGTTGTTA8110.11280213865065832No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8090.11252395828407222No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTGCCCCTCCGACATTCGGCCAAGGGA7980.11099396626784874No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7960.11071578590126266No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT7950.11057669571796962No Hit
GGATGGGACCGCACTTTGCAAAGTGGATGCAGCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATA7930.11029851535138352No Hit
GTGCCAGATGTGACATCCAGATGGCCCAGTCTCCGCCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCA7840.10904670370174613No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7790.10835125278528092No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAAAGTGCGGTCCCATCCAGGTT7720.10737762150222963No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC7690.10696035095235049No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7660.10654308040247136No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC7640.10626490003588526No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7550.10501308838624787No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC7520.10459581783636873No Hit
CATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTGCCCCTCCGACATTCGG7510.1044567276530757No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7440.1034830963700244No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7350.102231284720387No Hit
GCCCCTAACCTCCTGATCTATGCTGCATCCACTTTGCAAAGTGCGGTCCCATCCAGGTTCAGTGGCAGTGGAT7340.10209219453709396No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7330.10195310435380091No Hit
ATGTATTACTGTGCGAGACAGCGTTGTAGTGGTGGTAGCTGCTACTCGGC7280.1012576534373357No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGCG200.007174820652.08075743
TATAAGA603.2643948E-739.6621252
GTATCAA21250.036.5037461
TATCAAC23400.033.1244132
AATGGTA2950.033.0924870
ATCAACG24000.032.29633
TCAACGC24150.032.0957034
TTAACGG751.7268503E-632.0574335
CAACGCA24600.031.5129685
AACGCAG24700.031.2499056
GTGGTAT3000.030.6199191
GTGTTAT450.00494782930.2418981
TCTACCC450.004966383330.2187613
ACGCAGA26150.029.5171177
CGCAGAG26250.029.404678
AGAGTAC26450.029.05377611
AACTGTA1803.31931E-728.92528270
ACCCTAA853.9372044E-628.84122849
GCAGAGT26850.028.6209439
GTATAAG854.9323426E-628.0182271
Produced by FastQC (version 0.11.5)