FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655547
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAAGTTACCCGTGGACTTTTGGCCAGGGGACCAGAGTGGAGATGAAACGAACTGTGGCTGCACCAT23810.3632081300044085No Hit
GTAATAGACAGCCGCGTCTTCACCTTTAAGGCTGTTCATTTGCAGATATA20900.31881772016346654No Hit
GAGTTTACTCTCACCATCAGCAGCCTGCAGCCTGAAGACTTTGCAACTTATTACTGCCAACAGTATAAAAGTTAC19660.2999022190628589No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19550.2982242310619986No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18360.28007145177996395No Hit
GTCTATTACTGCGCCAGAGGGGGTTATTATGACTGGGCACTGGCCTTGGG18330.2796138186888202No Hit
CTTTTATACTGTTGGCAGTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTAAACTCTGTC17030.2597830514059251No Hit
GTATTACGCAGACTCCGTGAAGGGCCGATTCATTATCTCCAGAGACAATT16380.24986766776447764No Hit
GTGCAGTGACAGTCGGGGGTGACAGCATGAGGGAGATGGGGTAGGTGGAG13860.21142648810840414No Hit
CCTTTAAGGCTGTTCATTTGCAGATATAGGATATTCTTGGAATTGTCTCT12600.19220589828036738No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12230.18656175682292803No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12170.18564649064064057No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11450.174663296453191No Hit
GCCTTAAAGGTGAAGACGCGGCTGTCTATTACTGCGCCAGAGGGGGTTATTATGACTGGGCACTGGCCTTGGGGT11220.17115477608775573No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11130.16978187681432452No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11070.16886661063203706No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11060.1687140662683225No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10930.166730989540033No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC10810.16490045717545804No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10770.16429027972059976No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10130.1545274404428668No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9990.1523918193508627No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9720.14827312153056912No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9640.1470527666208525No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9630.14690022225713795No Hit
ATAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGAGCATCGACGATAATTTAGCCTGGTTTCAGAAGAAA9320.14217134698198602No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGACCTGGGTTTTC9130.13927300407140908No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9030.1377475604342633No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT8970.13683229425197585No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8930.13622211679711752No Hit
GTCTCCATCCTCACTGTCTGCATCTATAGGAGACAGAGTCACCATCACTT8850.1350017618874009No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8820.13454412879625718No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8790.13408649570511344No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8790.13408649570511344No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8760.13362886261396972No Hit
GCGTAATACTGATTACTTCCATCATTTGAAATAACAGCCACCCACTCCAGCCCCCTGCCTGGAGCCTGGCGGACC8600.13118815279453647No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8580.1308830640671073No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGA8490.1295101647936761No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC8380.12783217679281578No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT8380.12783217679281578No Hit
GGGCTGGAGTGGGTGGCTGTTATTTCAAATGATGGAAGTAATCAGTATTA8250.12584910006452626No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8250.12584910006452626No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8160.12447620079109507No Hit
CTCCAGCCCCCTGCCTGGAGCCTGGCGGACCCAATGCATAGCGTAGCGGC8130.12401856769995133No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG8090.12340839024509304No Hit
ATGCATTGGGTCCGCCAGGCTCCAGGCAGGGGGCTGGAGTGGGTGGCTGT7910.12066259169823063No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7800.11898460369737028No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7600.11593371642307874No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC7600.11593371642307874No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7520.11471336151336212No Hit
GAGTGGAGATGAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG7410.11303537351250177No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7300.11135738551164141No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT7270.1108997524204977No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC7210.10998448623821024No Hit
GTCATAATAACCCCCTCTGGCGCAGTAATAGACAGCCGCGTCTTCACCTT7170.10937430878335191No Hit
TAATAACCCCCTCTGGCGCAGTAATAGACAGCCGCGTCTTCACCTTTAAG7130.10876413132849361No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT7130.10876413132849361No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG7130.10876413132849361No Hit
AGTCAGAGCATCGACGATAATTTAGCCTGGTTTCAGAAGAAACCGGGGAGGGCCCCTAAGTCCCTGATTTTCG7070.10784886514620615No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7060.10769632078249157No Hit
GCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGTTGAGGGAACTGGGCAGGGCACAGT7020.10708614332763325No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA6950.10601833278163122No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG6930.10571324405420207No Hit
CTCCTGTGAAGCCTCTGTATTCACCTTCAGCCGCTACGCTATGCATTGGGTCCGCCAGGCTCCAGGCAGGGGG6760.10311998987105424No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC6750.10296744550733966No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA6740.1028149011436251No Hit
GTCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTAAACTCTGTCCCAGAGCCACTGCCGCTGAACTTTGATGGGAC6720.10250981241619594No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG6720.10250981241619594No Hit
GTCTGCATCTATAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGA6700.10220472368876678No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGACCTGGG6660.10159454623390848No Hit
GAGTAAACTCTGTCCCAGAGCCACTGCCGCTGAACTTTGATGGGACCCCA6620.10098436877905018No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG6590.10052673568790643No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)