FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655547
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAAGTTACCCGTGGACTTTTGGCCAGGGGACCAGAGTGGAGATGAAACGAACTGTGGCTGCACCAT22920.34963168163381114No Hit
GTAATAGACAGCCGCGTCTTCACCTTTAAGGCTGTTCATTTGCAGATATA19740.3011225739725756No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19010.28998683542141146No Hit
CTTTTATACTGTTGGCAGTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTAAACTCTGTC19000.2898342910576968No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18820.28708849251083446No Hit
GTCTATTACTGCGCCAGAGGGGGTTATTATGACTGGGCACTGGCCTTGGG18320.2794612743251056No Hit
GAGTTTACTCTCACCATCAGCAGCCTGCAGCCTGAAGACTTTGCAACTTATTACTGCCAACAGTATAAAAGTTAC18140.27671547577824324No Hit
GTATTACGCAGACTCCGTGAAGGGCCGATTCATTATCTCCAGAGACAATT17200.26237630558907293No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14360.21905370629413298No Hit
GAGTAAACTCTGTCCCAGAGCCACTGCCGCTGAACTTTGATGGGACCCCA12990.19815512846523592No Hit
CCTTTAAGGCTGTTCATTTGCAGATATAGGATATTCTTGGAATTGTCTCT12600.19220589828036738No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12460.1900702771883633No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11800.18000234918320118No Hit
GCCTTAAAGGTGAAGACGCGGCTGTCTATTACTGCGCCAGAGGGGGTTATTATGACTGGGCACTGGCCTTGGGGT11100.1693242437231808No Hit
GTGCAGTGACAGTCGGGGGTGACAGCATGAGGGAGATGGGGTAGGTGGAG10380.15834104953573122No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10370.15818850517201666No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10280.15681560589858545No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9890.15086637571371694No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9730.14842566589428371No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9450.14415442371027556No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9430.14384933498284638No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9180.14003572588998198No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8790.13408649570511344No Hit
CTCCAGCCCCCTGCCTGGAGCCTGGCGGACCCAATGCATAGCGTAGCGGC8600.13118815279453647No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8600.13118815279453647No Hit
GTCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTAAACTCTGTCCCAGAGCCACTGCCGCTGAACTTTGATGGGAC8570.13073051970339272No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC8570.13073051970339272No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8490.1295101647936761No Hit
GCGTAATACTGATTACTTCCATCATTTGAAATAACAGCCACCCACTCCAGCCCCCTGCCTGGAGCCTGGCGGACC8460.1290525317025324No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGACCTGGGTTTTC8420.12844235424767408No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8390.12798472115653034No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGA8320.12691691061052832No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8150.1243236564273805No Hit
GGGCTGGAGTGGGTGGCTGTTATTTCAAATGATGGAAGTAATCAGTATTA8070.12310330151766387No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC8030.12249312406280558No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8020.12234057969909098No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7860.11989986987965774No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC7800.11898460369737028No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7670.11700152696908078No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG7640.11654389387793704No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7640.11654389387793704No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7580.11562862769564958No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7440.1134930066036455No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7360.11227265169392887No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG7360.11227265169392887No Hit
ATGCATTGGGTCCGCCAGGCTCCAGGCAGGGGGCTGGAGTGGGTGGCTGT7320.11166247423907058No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7300.11135738551164141No Hit
GTCTCCATCCTCACTGTCTGCATCTATAGGAGACAGAGTCACCATCACTT7290.11120484114792685No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7260.11074720805678312No Hit
CTCCTGTGAAGCCTCTGTATTCACCTTCAGCCGCTACGCTATGCATTGGGTCCGCCAGGCTCCAGGCAGGGGG7160.10922176441963735No Hit
ATAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGAGCATCGACGATAATTTAGCCTGGTTTCAGAAGAAA7130.10876413132849361No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7070.10784886514620615No Hit
GTCATAATAACCCCCTCTGGCGCAGTAATAGACAGCCGCGTCTTCACCTT6990.10662851023648953No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT6970.10632342150906036No Hit
GAGTGGAGATGAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG6930.10571324405420207No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC6850.10449288914448544No Hit
TAATAACCCCCTCTGGCGCAGTAATAGACAGCCGCGTCTTCACCTTTAAG6820.10403525605334173No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6700.10220472368876678No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)