FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450573
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19320.42878734411516006No Hit
ATATATTACTGTGCGAGGCGGGCCACAATACAAAGTCATTTTTTCGGTAT17690.3926111862006822No Hit
GTAATATATAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCCTCAGGGAAA17650.3917234277242533No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13340.29606745188903905No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG11330.2514575884484867No Hit
GTCTACAACCTGAAGATTTTGGATCTTACTTCTGTCAACAGACTTACAGTCCCCCGTGGACGTTCGGCCAGGGGA9980.22149573986901122No Hit
GACTTACAGTCCCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT9480.21039875891364995No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9130.20263087224489706No Hit
CTGTAAGTCTGTTGACAGAAGTAAGATCCAAAATCTTCAGGTTGTAGACTGGTGATGGTGAGAGTGAATTCTGTC9120.20240893262578985No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8850.19641656290989473No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATCAGCACATATTTAAATTGGTATGTGCAGAAA8610.1910900120513213No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG8300.1842098838589973No Hit
GGTTAAGCCTTCGGAGACCCTGTCCCTCACCTGCGGTGTCAATGGTGGGT8240.18287824614435397No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7780.17266902366542158No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7600.1686741105214915No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT7590.1684521709023843No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7370.16356949928202533No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7190.15957458613809528No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7130.15824294842345193No Hit
GAATTCACTCTCACCATCACCAGTCTACAACCTGAAGATTTTGGATCTTACTTCTGTCAACAGACTTACAGTCCC6990.15513579375595077No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG6940.1540260956604146No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC6890.1529163975648785No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCGAACGTC6810.1511408806120207No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6570.14581432975344727No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC6560.14559239013434006No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6530.14492657127701838No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCGAACGTCCACGGGGGA6520.14470463165791114No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT6460.1433729939432678No Hit
CACCATCACCAGTCTACAACCTGAAGATTTTGGATCTTACTTCTGTCAACAGACTTACAGTCCCCCGTGGACGTT6380.14159747699040998No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC6310.14004389965665942No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC6210.13782450346558714No Hit
GATCTATGCTGCATCCAGTTTGCAGACTGGGGTCCCATCAAGTTTCAGTGGCAGTGGATCTGGGACAGAATTCA6150.1364928657509438No Hit
CTTCTACTGAGACGGTGACTCGACTCCTGAGGGACGGGTTGTAGTTGGAG5990.13294183184522818No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT5930.13161019413058483No Hit
CAGTAGAAGCGTCCAAGAACCAGTTTTCCCTGAGGCTGAGCTCTGTGACCGCCGCGGACACGGCTATATATTA5840.1296127375586198No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG5820.12916885832040534No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5770.12805916022486924No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT5740.12739334136754754No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT5680.12606170365290417No Hit
GTGCAGAAACCAGGGAAAGCCCCTAAATTCCTGATCTATGCTGCATCCAG5650.12539588479558253No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC5620.12473006593826085No Hit
CAGTAATATATAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCCTCAGGGAAAACTGGTTCTTGGACGCTTCTA5570.1236203678427247No Hit
CAGTTACACCAGGGGGGCGCAGGACTGGTTAAGCCTTCGGAGACCCTGTC5560.12339842822361748No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC5540.12295454898540302No Hit
GTTGTAGACTGGTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCACTGAAACTTGATGGGACCCCAGT5420.12029127355611632No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA5380.11940351507968741No Hit
ATGCTGCATCCAGTTTGCAGACTGGGGTCCCATCAAGTTTCAGTGGCAGTGGATCTGGGACAGAATTCACTCTCA5250.11651830003129347No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGCGGTCCCTTGG5230.11607442079307904No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCACTGAAACTTGATGGGACCCCA5200.11540860193575736No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG5200.11540860193575736No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC5130.11385502460200678No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC5090.11296726612557788No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA5090.11296726612557788No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4960.11008205107718395No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG4940.1096381718389695No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG4910.10897235298164781No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG4850.10764071526700444No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT4730.10497743983771775No Hit
GTCAATGGTGGGTCCTTCGCTACTTACTTTTGGACCTGGATCCGCCAGTC4680.10386774174218164No Hit
TATTTAAATTGGTATGTGCAGAAACCAGGGAAAGCCCCTAAATTCCTGATCTATGCTGCATCCAGTTTGCAGA4670.10364580212307441No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC4600.10209222478932381No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG4550.1009825266937877No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG4540.10076058707468047No Hit
ATATTACTGTGCGAGGCGGGCCACAATACAAAGTCATTTTTTCGGTATCGACGTCTGGGGCCAAGGGACCGCGGT4530.10053864745557324No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)