FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004685042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715163
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC38840.5430929732103031No Hit
GTATATGCATATGAGGCACAATAATAGTCAGCCTCGTCTTCAGCCCGGAG23630.33041418529761746No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCGCCGCCGAAATTGAC22650.31671101553072517No Hit
GTCCTGGGCCCAGTCTGACCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG22060.3084611480180043No Hit
GTAATATACGGCCGTGTCGTCAGATCTGAGTCCGGTCACATCCATGCTGGCTGTGTTGGTGGACGTGTCCCAGGT22050.3083213197550768No Hit
TGCATATACTGTCAATTTCGGCGGCGGGACCAAGCTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCA21320.29811385656137135No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20490.2865081107383911No Hit
CTATTATTGTGCCTCATATGCATATACTGTCAATTTCGGCGGCGGGACCA20460.2860886259496087No Hit
CAATAATAGTCAGCCTCGTCTTCAGCCCGGAGCCCAGAGATGGTCAGGGA20000.27965652585494494No Hit
CTCCTGCACTGCAACCAGCCGTGACATTGGTGATTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGT17670.24707654059284387No Hit
TCTCTGGGCTCCGGGCTGAAGACGAGGCTGACTATTATTGTGCCTCATAT16600.2321149164596043No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16260.22736075552007026No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGCAACCAGCCGTGACATT16130.22554298810201312No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15700.2195303727961318No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15530.21715329232636477No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT14560.20358995082239992No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13960.19520025504675156No Hit
GTATATTACTGTGCGACCTTAGGATTCTACGGTGGCCCTTCCGGCCTACT13910.19450111373211423No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGACCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC13910.19450111373211423No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13390.18723004405988566No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCGC13350.18667073100817577No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12560.17562429823690542No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12130.16961168293102413No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11930.16681511767247467No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11800.16499735025441753No Hit
GTCGATCACCATCTCCTGCACTGCAACCAGCCGTGACATTGGTGATTATAACTATGTCTCCTGGTACCAACAACA11560.16164147194415818No Hit
CCGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC11430.15982370452610103No Hit
GACTATTATTGTGCCTCATATGCATATACTGTCAATTTCGGCGGCGGGAC11330.15842542189682632No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG11200.15660765447876918No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10940.1529721196426549No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10770.15059503917288786No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGCGTGGATGGGACGGATCAACCCTAACAATGGTGG10650.1489171000177582No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10420.14570104997042632No Hit
ATAATAGTCAGCCTCGTCTTCAGCCCGGAGCCCAGAGATGGTCAGGGAGG10230.14304431297480436No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10040.14038757597918236No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9950.1391291216128351No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9630.134654617199156No Hit
GGCCTGGGCTCCGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGACCTGACTCAAC9540.13339616283280875No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9510.13297667804402633No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGG9490.1326970215181714No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCGGTGAGGCTATCCTGCAAGGCTTCTGGATACACCTTCAGCGG9170.12822251710449228No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC9090.1271038910010725No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT9020.12612509316058018No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC8960.12528612358301536No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC8950.12514629532008786No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8880.12416749747959557No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8620.12053196264348127No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8450.11815488217371424No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8400.11745574085907688No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGACCTGACTCAACCT8340.11661677128151204No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT8260.11549814517809226No Hit
GACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT8210.11479900386345492No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8160.11409986254881754No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8120.11354054949710764No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8110.11340072123418017No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8040.11242192339368787No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCCGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC7870.11004484292392083No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7860.10990501466099337No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7820.10934570160928347No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7730.10808724724293622No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG7710.10780759071708128No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT7600.10626947982487907No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC7590.1061296515619516No Hit
CATATGCATATACTGTCAATTTCGGCGGCGGGACCAAGCTGACCGTCCTC7470.10445171240682195No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGCGTGGATGGGACGGATCAACCCTAACAATGGTGGCACAAAGTATG7470.10445171240682195No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7390.10333308630340217No Hit
ACCATGGCCTGGGCTCCGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGACCTGACT7380.1031932580404747No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)