FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715163
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC35690.49904707038814927No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCGCCGCCGAAATTGAC26370.36872712933974494No Hit
GTAATATACGGCCGTGTCGTCAGATCTGAGTCCGGTCACATCCATGCTGGCTGTGTTGGTGGACGTGTCCCAGGT22040.30818149149214935No Hit
GTATATGCATATGAGGCACAATAATAGTCAGCCTCGTCTTCAGCCCGGAG21660.30286801750090536No Hit
GTCCTGGGCCCAGTCTGACCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG20780.2905631303632878No Hit
TGCATATACTGTCAATTTCGGCGGCGGGACCAAGCTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCA20550.28734708031595596No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19800.2768599605963955No Hit
CTATTATTGTGCCTCATATGCATATACTGTCAATTTCGGCGGCGGGACCA19640.27462270838955594No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18470.2582628016270417No Hit
CTCCTGCACTGCAACCAGCCGTGACATTGGTGATTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGT17550.2453986014377142No Hit
CAATAATAGTCAGCCTCGTCTTCAGCCCGGAGCCCAGAGATGGTCAGGGA16620.23239457298545926No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15660.2189710597444219No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15490.21659397927465485No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGCAACCAGCCGTGACATT15390.21519569664538016No Hit
TCTCTGGGCTCCGGGCTGAAGACGAGGCTGACTATTATTGTGCCTCATAT14950.20904325307657137No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT14550.20345012255947245No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14200.19855613335701094No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGACCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC13740.19212403326234717No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13730.1919842049994197No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13340.18653090274524828No Hit
GTATATTACTGTGCGACCTTAGGATTCTACGGTGGCCCTTCCGGCCTACT13270.185552104904756No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13180.18429365053840874No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12160.17003116771980653No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCGC12150.16989133945687906No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11970.16737443072418454No Hit
GTCGATCACCATCTCCTGCACTGCAACCAGCCGTGACATTGGTGATTATAACTATGTCTCCTGGTACCAACAACA11960.16723460246125707No Hit
CCGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC11640.16276009804757796No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11420.15968387626317357No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11100.15520937184949446No Hit
GACTATTATTGTGCCTCATATGCATATACTGTCAATTTCGGCGGCGGGAC11020.15409074574607468No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGCGTGGATGGGACGGATCAACCCTAACAATGGTGG10720.1498958978582505No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10370.14500190865578896No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10270.14360362602651422No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGG10100.14122654555674719No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG10030.1402477477162549No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9540.13339616283280875No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9500.13283684978109886No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9340.1305995975742593No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9270.129620799733767No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC9200.12864200189327468No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCGGTGAGGCTATCCTGCAAGGCTTCTGGATACACCTTCAGCGG9020.12612509316058018No Hit
ATAATAGTCAGCCTCGTCTTCAGCCCGGAGCCCAGAGATGGTCAGGGAGG8890.12430732574252304No Hit
GGCCTGGGCTCCGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGACCTGACTCAAC8880.12416749747959557No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8590.12011247785469886No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8580.1199726495917714No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGCGTGGATGGGACGGATCAACCCTAACAATGGTGGCACAAAGTATG8430.11787522564785931No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGACCTGACTCAACCT8290.11591762996687469No Hit
GACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT8240.11521848865223731No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8110.11340072123418017No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC8110.11340072123418017No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG8030.1122820951307604No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC8000.11186261034197799No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC7990.11172278207905051No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7950.11116346902734062No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7910.11060415597563074No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCCGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC7890.11032449944977578No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT7630.10668896461366151No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7460.10431188414389447No Hit
ACCATGGCCTGGGCTCCGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGACCTGACT7390.10333308630340217No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7340.1026339449887648No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7330.10249411672583733No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT7290.10193480367412742No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)