FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563808
Sequences flagged as poor quality0
Sequence length21-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT18910.3353978659401782No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16910.29992479709404624No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC16620.29478120211135705No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC16400.29087916453828255No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15760.27952778250752025No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTGCTGCTTGTATATGAACTGCAGTAATAAT14560.25824394119984106No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC14520.25753447982291844No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC14250.2527456155286906No Hit
GTAATAGACGGCCGTGTCTTCAGCTCTCAGGCTGTTCAGCTCCAAGTAGG13820.24511890572677222No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG13440.23837902264600713No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC13180.23376752369601No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG13000.2305749474998581No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC12700.22525398717293832No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCTCTGGAACCATCAGTGACGTT12150.21549889324025198No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTGCTGC11800.20929110619217892No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG11520.2043248765537204No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGTTCATAT10480.18587888075373177No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC10250.18179947783642658No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG10020.17772007491912142No Hit
GATTATTACTGCAGTTCATATACAAGCAGCAGCACTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT9840.17452749872296952No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT9820.17417276803450818No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9670.1715122878710483No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTGCTGCTTGTATATGAACTG9420.1670781542652818No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT9210.16335348203643793No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTG9040.1603382711845167No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG8980.15927407911913274No Hit
GTCGATCACCATCTCCTGCTCTGGAACCATCAGTGACGTTGGTGGTTATAACCATGTCTCCTGGTACCAACAGCA8780.15572677223451956No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8350.14810006243260118No Hit
GTCTATTACTGTGCGAGATATTGTAGTGGCGGAAGTTGTTATTCGCGCTGGTTCGACCCCTGGGGCCAGGGAACC8140.1443753902037573No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT8000.14189227538452806No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC7930.14065071797491344No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG7820.13869969918837619No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7800.13834496849991487No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7720.1369260457460696No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG7450.13213718145184175No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG7390.1310729893864578No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT7370.1307182586979965No Hit
GGTTATAACCATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCACTTAT7330.13000879732107384No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA7290.1292993359441512No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC7150.12681622112492194No Hit
GTTCATATACAAGCAGCAGCACTTATGTCTTCGGAACTGGGACCAAGGTC6690.11865741529031158No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6480.11493274306146772No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTC6460.11457801237300641No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC6400.11351382030762246No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG6390.1133364549633918No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6330.11227226289800783No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT6310.11191753220954652No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC6270.11120807083262386No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT6210.1101438787672399No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG6180.10961178273454791No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT6170.10943441739031728No Hit
GTTATAACCATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAA5990.10624184119416538No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG5940.10535501447301208No Hit
GTATATGAACTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA5920.10500028378455079No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC5860.10393609171916682No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA5850.10375872637493615No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA5810.1030492649980135No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC5810.1030492649980135No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG5790.10269453430955218No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG5750.10198507293262954No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG5730.10163034224416823No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT5710.1012756115557069No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC5680.10074351552301494No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACAAC200.007393347651.68566538
GTATAGA200.0075803651.3601651
GGTATCA5050.050.1736261
GTGGTAT1050.042.3925131
TAGTCAC602.9355215E-740.19996338
CGCGGTG4350.039.0201970
AACTGTA859.8924764E-538.4022270
GTATCAA13100.037.8993571
ATGGCAA2201.8189894E-1235.60933370
TGGTATC1250.035.60342
GGTAATC601.1056729E-535.4865861
TATCAAC14650.034.1171952
ATAGTCA652.3535335E-531.77819437
AATAGTC652.3679213E-531.7498836
ATCAACG15950.031.5511193
TCAACGC16200.031.0642194
AACGCAG16250.030.9686366
ATGCGCG450.00441806230.94899749
CAACGCA16400.030.6853875
GTAATCA703.1400807E-530.46467662
Produced by FastQC (version 0.11.5)