FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936649
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACCAGTCCGCTCACTTTCGGCGGAGGGACCAACGTGGAGATCAAACGAACTGTGGCTGCACCAT72120.7699789355457595No Hit
ATTTTATACAGCTCCGACAGTAAGAATTACTTAGCTTGGTTCCAGCAGAA57800.617093489663684No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48650.5194048143968553No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43390.46324717156586936No Hit
CTCCTAAGCTGCTCATTAACTGGGCATCTACCCGGCAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTG42460.4533181586698966No Hit
GTATAATATTGCAGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCT40920.4368765674228019No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGAACCAAGCTAAGTAATTCTTA40770.4352751137299031No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA36540.39011411959015596No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36320.387765320840571No Hit
GTATAAAATACTCCGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA35730.3814662696485023No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33070.35306715749442963No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30940.33032651505526617No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT30640.3271236076694685No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30550.3261627354537292No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30220.32263953732935174No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29250.31228347011527263No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27420.2927457350619069No Hit
GGTATAATATTGCAGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAAT27190.29029017273279534No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGCGACAGATTTCACTCTCAC26050.2781191246667642No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25510.2723538913723284No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25080.267763057452685No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24860.2654142587031001No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24650.26317222353304176No Hit
GCTCATTAACTGGGCATCTACCCGGCAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGCGACAGATTT24560.26221135131730244No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23960.2558055365457071No Hit
GTTAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGAACCAAGCTA23620.2521755748418031No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22040.235306929276602No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21650.23114314967506508No Hit
GTCAGGGACCCCGGATTGCCGGGTAGATGCCCAGTTAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGAA21490.22943493240263962No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTAACTGGGCATCTACCCGGCAA21400.22847406018690034No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20910.22324264479009745No Hit
ATTCAGTGGCAGCGGGTCTGCGACAGATTTCACTCTCACCATCGGCAGCC20120.21480832200749694No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC20060.2141677405303374No Hit
GTTTATTATTGTCTGCAATATTATACCAGTCCGCTCACTTTCGGCGGAGGGACCAACGTGGAGATCAAACGAA19480.20797545291779523No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCGGAGTATTTTATA19150.2044522547934178No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18990.2027440375209924No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACGTTGGTCCCTCCGCCGAAAGTG18630.1989005486580352No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18610.198687021498982No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCGGAGTATTTTATACAGCTCCGA18590.19847349433992886No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18370.19612469559034387No Hit
ATACCAGTCCGCTCACTTTCGGCGGAGGGACCAACGTGGAGATCAAACGA18270.19505705979507798No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16900.1804304493999353No Hit
GTCCTGGTTTCTGCTGGAACCAAGCTAAGTAATTCTTACTGTCGGAGCTG16400.17509227042360587No Hit
CAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAATCTGTCGCAGACCCGCTGCCA16340.17445168894644633No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16340.17445168894644633No Hit
GAATTACTTAGCTTGGTTCCAGCAGAAACCAGGACAGCCTCCTAAGCTGC16070.17156907229922844No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15860.16932703712917005No Hit
TTATTATTGTCTGCAATATTATACCAGTCCGCTCACTTTCGGCGGAGGGA15550.1660173661638458No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15490.16537678468668626No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15470.16516325752763308No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15460.1650564939481065No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15410.16452267605047355No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15390.16430914889142037No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15310.16345504025520766No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15240.16270769519852155No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC14910.15918449707414412No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14820.1582236248584048No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14790.15790333411982502No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14580.15566129894976666No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14510.15491395389308055No Hit
ATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAATCTGTCGCAGACCCGCTGCCA14450.154273372415921No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14350.15320573662065512No Hit
GGCTGAAGATGTGGCAGTTTATTATTGTCTGCAATATTATACCAGTCCGC14340.15309897304112852No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14330.15299220946160194No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14210.15171104650728287No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13700.14626610395142683No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13540.14455788667900143No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13400.14306319656562919No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG13230.14124821571367716No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG13160.14050087065699104No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT13090.1397535256003049No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13020.1390061805436188No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCGGAGTATTTTATACAGCTCCGACAGTAAGAATTA12980.13857912622551244No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12720.13580327315782112No Hit
GACTGGTATAATATTGCAGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAAT12620.13473563736255526No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG12440.13281389293107662No Hit
CTTTCGGCGGAGGGACCAACGTGGAGATCAAACGAACTGTGGCTGCACCA12340.13174625713581076No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC12340.13174625713581076No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12040.12854334975001308No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11710.12502015162563565No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACGTTGGTCC11600.12384575225084316No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11400.12171048066031138No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11390.1216037170807848No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11330.12096313560362526No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG11250.12010902696741256No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11230.11989549980835938No Hit
TCTTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAATCTGTCGCAGACCCGCTGCCACTGAATCGGTCAGGGACC10750.1147708479910831No Hit
CACCATCGGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCTGCAATATTATACCAGTCCGCTCACTTT10750.1147708479910831No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA10680.11402350293439698No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC10660.1138099757753438No Hit
GATTTCACTCTCACCATCGGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCTGCAATATTATACCAGT10580.1129558671391311No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10570.11284910355960451No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10560.1127423399800779No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10280.10975295975333343No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG10190.10879208753759413No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC10170.10857856037854094No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10150.10836503321948777No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10080.10761768816280165No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10070.10751092458327505No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10000.10676357952658894No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9970.10644328878800917No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9910.10580270731084962No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9880.10548241657226985No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA9850.10516212583369011No Hit
AAGTAATTCTTACTGTCGGAGCTGTATAAAATACTCCGGCTGGACTTGCA9850.10516212583369011No Hit
ATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAG9830.10494859867463692No Hit
CCACTGAATCGGTCAGGGACCCCGGATTGCCGGGTAGATGCCCAGTTAAT9780.10441478077700397No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTACCCCTATTGCGGTCCT9740.10398772645889762No Hit
GATTCACTTTATTTATTCATTCTCCTCCAACATTAGCATAATTAAAGCCA9630.10281332708410515No Hit
GGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGC9550.10195921844789242No Hit
CATTAACTGGGCATCTACCCGGCAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGCGACAGATTTCA9520.10163892770931265No Hit
GTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGG9490.10131863697073289No Hit
AAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTC9420.10057129191404678No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACGTTGGTCCCTCCGC9420.10057129191404678No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACGTTGGTCCCTCCGCCGAAAGTGAGCGGACTGGT9390.10025100117546701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2750.052.3135261
TGGTATC2850.050.4590952
CCGTATT305.3449906E-447.57622547
CGCGTAT1100.046.73441339
AAGGGGG7800.045.8953369
TCGCGTA1050.045.4389538
GGTATCA13000.045.019811
GCTTGAA6600.043.44213556
CTTGAAA6650.043.35547357
ACGTCGA658.493074E-443.303270
GCCGTCT5500.043.23302546
ATGCCGT6450.043.1181844
GACAGGT1150.042.6468931
TATGCCG7100.042.4268743
CCGTCTT5750.042.19804847
TGCTTGA6100.041.94859355
TCGTATG6250.041.9163340
GTATCAA39900.041.136261
GTATGCC7350.040.78078542
ATCGCGT1250.040.67767337
Produced by FastQC (version 0.11.5)