FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936649
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77410.8264568691153249No Hit
ATATTATACCAGTCCGCTCACTTTCGGCGGAGGGACCAACGTGGAGATCAAACGAACTGTGGCTGCACCAT69630.7433948042436388No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47950.5119313638299939No Hit
ATTTTATACAGCTCCGACAGTAAGAATTACTTAGCTTGGTTCCAGCAGAA47840.5107569644552015No Hit
GTATAATATTGCAGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCT46210.4933545009923675No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGAACCAAGCTAAGTAATTCTTA37940.4050610207238784No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36780.39267644549879405No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34870.37228460180921563No Hit
CTCCTAAGCTGCTCATTAACTGGGCATCTACCCGGCAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTG34520.368547876525785No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34310.36630584135572664No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34100.3640638061856683No Hit
GGTATAATATTGCAGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAAT31510.33641203908828177No Hit
GTATAAAATACTCCGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA30510.32573568113562285No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29480.31473903244438417No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28730.30673176397989No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28240.30150034858308716No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26490.2828167221659341No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25320.27032538336132317No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25240.2694712747251105No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24130.2576205173976591No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22940.24491565143399502No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGCGACAGATTTCACTCTCAC22870.24416830637730888No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22690.24224656194583027No Hit
ATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAATCTGTCGCAGACCCGCTGCCA22540.24064510825293145No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22410.2392571817190858No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22250.2375489644466604No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21690.2315702039931714No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21620.23082285893648527No Hit
GTTAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGAACCAAGCTA21610.2307160953569587No Hit
CAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAATCTGTCGCAGACCCGCTGCCA21380.22826053302784713No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC20870.22281559047199112No Hit
TCTTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAATCTGTCGCAGACCCGCTGCCACTGAATCGGTCAGGGACC20870.22281559047199112No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACGTTGGTCCCTCCGCCGAAAGTG19860.21203246893980562No Hit
GTTTATTATTGTCTGCAATATTATACCAGTCCGCTCACTTTCGGCGGAGGGACCAACGTGGAGATCAAACGAA19540.20861603439495477No Hit
GCTCATTAACTGGGCATCTACCCGGCAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGCGACAGATTT19450.20765516217921548No Hit
GTCAGGGACCCCGGATTGCCGGGTAGATGCCCAGTTAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGAA19430.20744163502016227No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19370.20680105354300274No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18920.2019966924643063No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18350.1959111684312907No Hit
ATTCAGTGGCAGCGGGTCTGCGACAGATTTCACTCTCACCATCGGCAGCC17900.1911068073525942No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17710.189078299341589No Hit
ATACCAGTCCGCTCACTTTCGGCGGAGGGACCAACGTGGAGATCAAACGA17560.18747684564869016No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16760.17893575928656305No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16590.17712077843461105No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16460.1757328519007654No Hit
GTCCTGGTTTCTGCTGGAACCAAGCTAAGTAATTCTTACTGTCGGAGCTG16420.17530579758265902No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCGGAGTATTTTATACAGCTCCGA16280.1738111074692868No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTAACTGGGCATCTACCCGGCAA16270.1737043438897602No Hit
TTATTATTGTCTGCAATATTATACCAGTCCGCTCACTTTCGGCGGAGGGA15720.1678323470157978No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCGGAGTATTTTATA15400.16441591247094697No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15210.16238740445994176No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15020.16035889644893658No Hit
GGCTGAAGATGTGGCAGTTTATTATTGTCTGCAATATTATACCAGTCCGC14830.1583303884379314No Hit
GAGTGAAATCTGTCGCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG14760.15758304338124526No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14680.15672893474503255No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14430.15405984525686783No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14340.15309897304112852No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14110.15064341071201698No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13990.14936224775769794No Hit
GACTGGTATAATATTGCAGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAAT13570.1448781774175812No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13550.144664650258528No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC13450.14359701446326212No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13380.142849669406576No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13190.1408211613955708No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13010.13889941696409222No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12760.13623032747592748No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12630.13484240094208183No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12590.13441534662397547No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT12410.13249360219249687No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12260.13089214849959804No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12220.13046509418149166No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12150.12971774912480555No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12120.1293974583862258No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12010.12822305901143333No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11870.12672836889806108No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11810.12608778742090154No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG11740.12534044236421543No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACGTTGGTCCCTCCGCCGAAAGTGAGCGGACTGGT11710.12502015162563565No Hit
CTTTCGGCGGAGGGACCAACGTGGAGATCAAACGAACTGTGGCTGCACCA11680.12469986088705588No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11680.12469986088705588No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG11680.12469986088705588No Hit
GAATTACTTAGCTTGGTTCCAGCAGAAACCAGGACAGCCTCCTAAGCTGC11600.12384575225084316No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG11450.12224429855794433No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG11260.12021579054693915No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11090.11840080969498713No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10800.11530466588871606No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG10610.11327615787771086No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT10530.11242204924149814No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCGGAGTATTTTATACAGCTCCGACAGTAAGAATTA10380.11082059554859931No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC10220.1091123782761739No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10180.10868532395806754No Hit
CACCATCGGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCTGCAATATTATACCAGTCCGCTCACTTT10160.10847179679901436No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10040.10719063384469528No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC10030.1070838702651687No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC9970.10644328878800917No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9960.10633652520848258No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA9920.10590947089037624No Hit
GATTTCACTCTCACCATCGGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCTGCAATATTATACCAGT9920.10590947089037624No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9880.10548241657226985No Hit
ATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAG9860.10526888941321669No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG9730.10388096287937104No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9420.10057129191404678No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT9410.10046452833452019No Hit
GGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACA9380.10014423759594042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)