FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055457
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40550.38419376630218No Hit
GTAATATACGGCCGTGTCTGCGGCGGTCACAGAGCTCAACTTCAGGGAGA37820.35832819337973976No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37450.3548226029103981No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT36080.3418424436049976No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG28590.270877923022918No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28010.265382673098004No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24480.2319374451067168No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24190.2291898201442598No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGTACCTATTTACAGTGGTATCAGCAACAA22360.21185135917427236No Hit
GTATATTACTGTGCGAGACGGGGACGCGGAGGCTTTAATTACTTTTTTGA22240.21071441091394533No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22080.20919847990017595No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21810.2066403463144401No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21780.20635610924935832No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21470.20341899291018012No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21360.2023767903382137No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG21300.20180831620805018No Hit
AATCTATGGTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACTGGCAGTGGATCTGGGACAGATTTCA20860.1976395059201843No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20760.19669204903657844No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20250.19186001893018853No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGTAC19980.1893018853444527No Hit
CCATAGATTAGGAGCTTAGGGGCTCTCCCTGGTTGTTGCTGATACCACTG18400.17433206658347994No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17390.16476275205906066No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17190.16286783829184895No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16870.15983597626431015No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16790.15907801075742545No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16740.1586042823156225No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT16680.158035808185459No Hit
CACCATCACCAGTCTGCAACCTGAAGATTTTGTAACTTACTTCTGTCAAG16670.1579410624970984No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16460.1559514030415261No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC16310.15453021771611727No Hit
GGGCAAAAGTGTACAGAGAGCTGTAACTCTCTTGACAGAAGTAAGTTACA16190.15339326945579024No Hit
GTGCAGCCACAGTTCGTCTGATTTCCAGCTTGGTCCCCGGGGCAAAAGTG16140.1529195410139873No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATTTCCAGCTTGGTCCCCGGGGCAAAAGTGTACAGAGAG16130.15282479532562673No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15980.1514036100002179No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC15880.15045615311661203No Hit
CTTTTGCCCCGGGGACCAAGCTGGAAATCAGACGAACTGTGGCTGCACCA15710.14884547641448206No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14220.1347283688487546No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13960.13226498095137937No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13820.13093854131433114No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13810.13084379562597054No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC13720.12999108443072527No Hit
ATATTATAGTGGGAATAGCAATTACAACCCCTCCCTCAAGAGTCGAGTCACCATTTCAGTAGACACGTCCAAGAA13600.12885413617039823No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13300.1260117655195806No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13300.1260117655195806No Hit
CTGTACACTTTTGCCCCGGGGACCAAGCTGGAAATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG13240.1254432913894171No Hit
GATTTCACTCTCACCATCACCAGTCTGCAACCTGAAGATTTTGTAACTTACTTCTGTCAAGAGAGTTACAGCTCT13230.12534854570105652No Hit
GAGTTACAGCTCTCTGTACACTTTTGCCCCGGGGACCAAGCTGGAAATCAGACGAACTGTGGCTGCACCAT13190.12496956294761416No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13170.12478007157089299No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13120.12430634312909006No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13110.12421159744072946No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13010.12326414055712359No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12860.1218429552317148No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12750.12080075265974834No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCAGTGAACCTTGATGGGACCCCA12710.12042176990630599No Hit
TCTATGGTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACTGGCAGTGGATCTGGGACAGATTTCACT12570.11909533026925777No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12530.11871634751581543No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12490.11833736476237308No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA12180.11540024842319489No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12150.11511601135811313No Hit
ACCAGGGAGAGCCCCTAAGCTCCTAATCTATGGTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACTGG12120.11483177429303136No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12020.11388431740942549No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11770.11151567520041082No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11560.10952601574483849No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11410.1081048304194297No Hit
GTACACTTTTGCCCCGGGGACCAAGCTGGAAATCAGACGAACTGTGGCTG11380.10782059335434793No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT11350.10753635628926617No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11320.10725211922418441No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11260.10668364509402088No Hit
CAACCAGGGAGAGCCCCTAAGCTCCTAATCTATGGTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCA11190.10602042527549677No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11100.10516771408025148No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11060.10478873132680916No Hit
CAGTAATATACGGCCGTGTCTGCGGCGGTCACAGAGCTCAACTTCAGGGAGAACTGGTTCTTGGACGTGTCTA11040.10459923995008798No Hit
GTGTACAGAGAGCTGTAACTCTCTTGACAGAAGTAAGTTACAAAATCTTC10990.10412551150828504No Hit
CCATTAGTACCTATTTACAGTGGTATCAGCAACAACCAGGGAGAGCCCCTAAGCTCCTAATCTATGGTGCGTCCA10990.10412551150828504No Hit
ATATTACTGTGCGAGACGGGGACGCGGAGGCTTTAATTACTTTTTTGACTCCTGGGGCCAGGGAACCCTGGTCAC10960.10384127444320328No Hit
GCCACAGTTCGTCTGATTTCCAGCTTGGTCCCCGGGGCAAAAGTGTACAG10880.10308330893631858No Hit
AAAGTGTACAGAGAGCTGTAACTCTCTTGACAGAAGTAAGTTACAAAATC10760.10194636067599153No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10730.10166212361090979No Hit
GTGGGGTCCCATCAAGGTTCACTGGCAGTGGATCTGGGACAGATTTCACT10650.10090415810402509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT554.1836756E-1156.2822939
TACTGAA10050.052.6821170
CGTCTTA200.00687076352.65590348
CCGTCTT6050.050.37814747
CGTATGC7100.050.10484741
ATCTCGT6700.049.09218237
TCGTATG6600.049.0789540
ACCGATC7550.048.82530227
GCCGTCT6200.048.46543546
CCGATCA7700.048.35013628
TATCTCG6800.048.24944336
GTATGCC7500.047.52716442
CGATCAG7700.047.50493629
CTTGAAA6900.047.46965457
CTTATAC7750.047.4412881
ATGCCGT6900.047.3717544
GCTTGAA6900.047.3451356
AAAGGGG7050.046.9826668
TGCTTGA6800.046.8319755
CCCACGA7850.046.83028819
Produced by FastQC (version 0.11.5)