FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055457
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70510.6680518486304985No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40190.38078292152119886No Hit
GTAATATACGGCCGTGTCTGCGGCGGTCACAGAGCTCAACTTCAGGGAGA36270.3436426116838488No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31580.29920688384273353No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG29300.2776048668965197No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25810.24453862165867488No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25550.2420752337612996No Hit
GTATATTACTGTGCGAGACGGGGACGCGGAGGCTTTAATTACTTTTTTGA23460.22227338489393694No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22060.2090089885234548No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21910.20758780319804598No Hit
AATCTATGGTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACTGGCAGTGGATCTGGGACAGATTTCA21480.20351373859854072No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGTACCTATTTACAGTGGTATCAGCAACAA21270.2015240791429684No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20700.19612357490641497No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19460.18437510954970218No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGTAC19340.18323816128937512No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19200.1819117216523269No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18650.17670070879249464No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG17920.1697842735421718No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17610.1668471572029936No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17350.16438376930561832No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16770.15888851938070428No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT16530.1566146228600502No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16520.1565198771716896No Hit
CCATAGATTAGGAGCTTAGGGGCTCTCCCTGGTTGTTGCTGATACCACTG16500.15633038579496844No Hit
CACCATCACCAGTCTGCAACCTGAAGATTTTGTAACTTACTTCTGTCAAG16380.1551934375346414No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16010.15168784706529967No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15980.1514036100002179No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC15840.1500771703631697No Hit
GTGCAGCCACAGTTCGTCTGATTTCCAGCTTGGTCCCCGGGGCAAAAGTG15790.14960344192136676No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15760.149319204856285No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15510.14695056264727033No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15210.14410819199645272No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATTTCCAGCTTGGTCCCCGGGGCAAAAGTGTACAGAGAG14800.14022361877366865No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC14800.14022361877366865No Hit
CTTTTGCCCCGGGGACCAAGCTGGAAATCAGACGAACTGTGGCTGCACCA14550.13785497656465398No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14380.136244299862524No Hit
GGGCAAAAGTGTACAGAGAGCTGTAACTCTCTTGACAGAAGTAAGTTACA14120.13378091196514874No Hit
CTGTACACTTTTGCCCCGGGGACCAAGCTGGAAATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG13520.12809617066351353No Hit
ATATTATAGTGGGAATAGCAATTACAACCCCTCCCTCAAGAGTCGAGTCACCATTTCAGTAGACACGTCCAAGAA13460.12752769653335No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13290.12591701983122003No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13160.12468532588253241No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA13090.12402210606400829No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC13090.12402210606400829No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13060.12373786899892653No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13010.12326414055712359No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCAGTGAACCTTGATGGGACCCCA12960.12279041211532066No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12880.12203244660843597No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12640.11975855008778187No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12490.11833736476237308No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12400.1174846535671278No Hit
GAGTTACAGCTCTCTGTACACTTTTGCCCCGGGGACCAAGCTGGAAATCAGACGAACTGTGGCTGCACCAT12390.11738990787876721No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12250.11606346824171898No Hit
ACCAGGGAGAGCCCCTAAGCTCCTAATCTATGGTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACTGG12160.11521075704647371No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11940.11312635190254079No Hit
GTACACTTTTGCCCCGGGGACCAAGCTGGAAATCAGACGAACTGTGGCTG11920.11293686052581961No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11870.11246313208401668No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11820.11198940364221377No Hit
GATTTCACTCTCACCATCACCAGTCTGCAACCTGAAGATTTTGTAACTTACTTCTGTCAAGAGAGTTACAGCTCT11800.11179991226549259No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11800.11179991226549259No Hit
TCTATGGTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACTGGCAGTGGATCTGGGACAGATTTCACT11710.1109472010702473No Hit
GTGGGGTCCCATCAAGGTTCACTGGCAGTGGATCTGGGACAGATTTCACT11710.1109472010702473No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11410.1081048304194297No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11390.10791533904270852No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11000.10422025719664563No Hit
CAGTAATATACGGCCGTGTCTGCGGCGGTCACAGAGCTCAACTTCAGGGAGAACTGGTTCTTGGACGTGTCTA10940.10365178306648211No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10910.10336754600140034No Hit
ATATTACTGTGCGAGACGGGGACGCGGAGGCTTTAATTACTTTTTTGACTCCTGGGGCCAGGGAACCCTGGTCAC10800.10232534342943388No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10800.10232534342943388No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC10710.1014726322341886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTGAA9750.062.39640470
TCTACTG9650.041.0981768
GTCTACT9500.039.883667
TGTCTAC9550.039.54846666
CCGTGTC10350.035.77575312
GTGTCTA10500.035.67732665
GTATCAA22600.035.638551
GCCGTGT10500.035.2646711
ACGTGTC10450.035.14086563
GACGTGT10600.034.5098162
CGGCGGT10600.034.35219621
TGCGGCG10650.034.17632319
TTGGACG10900.033.54156559
TGGACGT10900.033.3215260
GTCTGCG11150.032.62672416
GGACGTG11300.032.5950661
CAACTTC11400.032.45282737
AGCTCAA11200.031.9300333
GGCCGTG11500.031.90546810
TACGGCC11550.031.7673477
Produced by FastQC (version 0.11.5)