FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9502
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT385140.52830982950958No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT5205.472532098505578No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC4544.777941486002947No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT3023.1782782572090085Illumina PCR Primer Index 7 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCG2923.0730372553146705No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGT2772.915175752473164No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCAGCTCGTATGCCGT1531.610187328983372No Hit
TTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC961.0103136181856451RNA PCR Primer, Index 41 (95% over 22bp)
AGAGACAGCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCG940.9892654178067775No Hit
GAGACAGCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTC780.8208798147758366No Hit
GTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTCGACGTCA760.799831614396969No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTCGACGT720.7577352136392338No Hit
TATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCT700.7366870132603662No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG550.5788255104188592Illumina PCR Primer Index 7 (96% over 27bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG540.5683014102294254No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCGCGTATGCCGT490.5156809092822564No Hit
GAGACAGCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT460.48410860871395495No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCC450.47358450852452116No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATATCGTATGCCGT420.44201220795621976No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGTCTT400.4209640075773522No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGC290.3051989054935803No Hit
AGAGACAGCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCT280.29467480530414647No Hit
GCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCG260.27362660492527885No Hit
GTGTATAAGAGACAGATACACATCTCCGAGCCCACGAGACTCGACGTCAT260.27362660492527885No Hit
AGAGACAGTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTC260.27362660492527885No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGTCTTCT250.26310250473584507RNA PCR Primer, Index 7 (95% over 21bp)
CACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTG250.26310250473584507RNA PCR Primer, Index 41 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCAGGTCGTATGCCGT230.24205430435697747No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCAGCTCGTATGCC230.24205430435697747No Hit
GAGACAGTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCG220.2315302041675437No Hit
CTTATACACATCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCA220.2315302041675437No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCAGCTCGTATGCCGTCTT210.22100610397810988No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCG200.2104820037886761No Hit
TAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCT170.17890970322037464No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGACGTCAGCTCGTATGCCG170.17890970322037464No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCCTCTCGTATGCCGT160.16838560303094086No Hit
GATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTG140.14733740265207323Illumina PCR Primer Index 7 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGGCGTATGCCGT130.13681330246263942No Hit
GTATAAGAGACAGATACACATCTCCGAGCCCACGAGACTCGACGTCATCT130.13681330246263942No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG120.12628920227320564Illumina PCR Primer Index 7 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCCT110.11576510208377185No Hit
GAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT110.11576510208377185No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGG100.10524100189433805No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCGCGTATGCCGTCTT100.10524100189433805No Hit
CAGATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT100.10524100189433805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCAGC453.5470293E-1061.4932433
CAGCTCG409.711584E-960.8543936
TCAGCTC409.711584E-960.8543935
GTCAGCT401.0135409E-860.5324134
AGCTCGT352.7912574E-759.61245737
AGGGGGG5550.056.45689870
GTGTATA252.4718477E-455.052631
TGTATAA252.4718477E-455.052632
GCTCGTA452.4614565E-854.09279338
AAGGGGG5850.051.08669369
CTCTTAT900.049.7002941
TTATACA7200.046.832972
CTTATAC7200.046.832971
TATACAC7250.046.5099833
ACGTCAG604.5602064E-946.1199332
AAAGGGG6600.044.57386868
CGTCATG703.43789E-1044.4727933
AAAAGGG6750.043.58333267
AAAAAGG6800.043.55500466
TTGAAAA7900.043.1953358
Produced by FastQC (version 0.11.5)