FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1067970
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATGATAGTGTTCCGTGGACATTCGGCCAAGGGACCAATGTGGAAATTAAACGAACTGTGGCTGCACCAT56200.5262320102624606No Hit
CTATCATAGTGTTGGCAGTAATAACTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC50470.4725788177570531No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49910.46733522477223144No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48970.45853347940485223No Hit
GTATTAGTAGTTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGTCTA46700.43727820069852147No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32660.305813833721921No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32420.3035665795855689No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32210.30160023221626076No Hit
CACTATGATAGTGTTCCGTGGACATTCGGCCAAGGGACCAATGTGGAAATTAAACGAACTGTGGCTGCACCAT31950.29916570690187927No Hit
ATCATAGTGTTGGCAGTAATAACTTGCAAAATCATCAGGCTGCAGGCTGC29870.2796895043868273No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28120.2633032763092596No Hit
ACCTTAGAAAGTGGGGTCCCATCCAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC27570.2581533189134526No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27440.25693605625626187No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGCAGATACA27140.2541269885858217No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC27020.25300336151764563No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26670.24972611590213212No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26220.24551251439647181No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25810.2416734552468702No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25570.2394262011105181No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24910.2332462522355497No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAAGTTATTACTGCCAACACTATGATAGTGTT24610.2304371845651095No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22870.21414459207655645No Hit
GTGTATTACTGTGCGGGGTGTAGTGGTGGTATTTGCACACGCACAAATCC22530.21096098205005762No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21660.20281468580578108No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20250.18961206775471223No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20200.18914388980963887No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19710.1845557459479199No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19520.1827766697566411No Hit
CTACTAATACTTTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACATATGCAGACAAGGTGGAAGGAGA19510.18268303416762643No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT18860.17659672088167272No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18810.17612854293659935No Hit
GTGCAGCCACAGTTCGTTTAATTTCCACATTGGTCCCTTGGCCGAATGTC18020.1687313314044402No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17700.16573499255597068No Hit
TTCTAAGGTAGACGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTT17560.16442409430976526No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17250.1615213910503104No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCC17130.16039776398213434No Hit
ATAGTGTTCCGTGGACATTCGGCCAAGGGACCAATGTGGAAATTAAACGA16950.1587123233798702No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16530.15477962864125397No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16280.15243873891588713No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16210.15178328979278444No Hit
CCTTCCACCTTGTCTGCATATGTGGGAGACAGAGTCACCATCACTTGCCG15850.14841240858825622No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15770.14766332387613884No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15650.14653969680796275No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATTTCCACATTGGTCC15630.14635242562993342No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT15070.14110883264511176No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14770.13829976497467159No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14180.1327752652228059No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14110.13211981609970316No Hit
GAACACTATCATAGTGTTGGCAGTAATAACTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATT13980.13090255344251242No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13250.12406715544444132No Hit
GTCAAAGTATTAGTAGTTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGG13240.12397351985542664No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13160.12322443514330926No Hit
TCTATAAGGCGTCTACCTTAGAAAGTGGGGTCCCATCCAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT13090.12256898602020656No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13080.1224753504311919No Hit
ATGATAGTGTTCCGTGGACATTCGGCCAAGGGACCAATGTGGAAATTAAA13050.12219444366414786No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12950.12125808777400116No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12750.1193853759937077No Hit
GTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCCAACCAACTACTA12720.11910446922666366No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12690.11882356245961966No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12640.11835538451454629No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12630.11826174892553161No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGCAGATA12470.11676357950129686No Hit
GTATTACTGTGCGGGGTGTAGTGGTGGTATTTGCACACGCACAAATCCTT12470.11676357950129686No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12450.11657630832326751No Hit
GTGTAGTGGTGGTATTTGCACACGCACAAATCCTTCTGACATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTC12330.11545268125509142No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12310.11526541007706209No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC12120.11348633388578332No Hit
CCCCTAAACTCCTGATCTATAAGGCGTCTACCTTAGAAAGTGGGGTCCCA12110.11339269829676864No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12080.11311179152972463No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12000.11236270681760722No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11990.11226907122859256No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11940.1118008932835192No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11910.11151998651647518No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11810.11058363062632845No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11750.1100218170922404No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11560.10824274090096163No Hit
CTCCTGATCTATAAGGCGTCTACCTTAGAAAGTGGGGTCCCATCCAGGTT11500.10768092736687361No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11480.10749365618884425No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11360.10637002912066819No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11290.10571457999756548No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11280.1056209444085508No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11280.1056209444085508No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTGGATGGGACCCCA11270.10552730881953612No Hit
CCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCCAACCAACTACTAATACTTTGA11180.10468458851840406No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC10990.10290551232712529No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10940.10243733438205192No Hit
GTCTCCTTCCACCTTGTCTGCATATGTGGGAGACAGAGTCACCATCACTT10890.10196915643697856No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAAGTTATTACTGCCAACACTATGATAGTGTTCCGTGGACATT10870.10178188525894923No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAAAGTATTAGTAGTTGGTTGGCCTGGTATCAGCAAAAA10720.10037735142372914No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10690.10009644465668513No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10680.10000280906767045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG3050.057.77903740
CGTATGC3200.056.2143241
TAGCGCT3250.054.8401229
CTAGCGC3250.054.81952728
GACTAGC3250.054.77325426
ACTAGCG3300.053.96106727
CGTTACG200.00681464152.76531255
GTCGTAT200.007347677451.7699339
TGTCGTA200.007382076751.70881338
GATCGAT200.007470685551.55334
ATCTCGT2950.051.37300537
TAGGTCG200.007633303351.272985
ATCTCCG3500.050.78466410
TCTCCGA3500.050.78466411
ATGCCGT3400.050.02116444
CTTATAC3650.049.662021
CACGAGA3600.049.3855421
ACGAGAC3600.049.3855422
GTATGCC3600.049.05617542
CGCTCAT3250.048.56942432
Produced by FastQC (version 0.11.5)