FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1067970
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATGATAGTGTTCCGTGGACATTCGGCCAAGGGACCAATGTGGAAATTAAACGAACTGTGGCTGCACCAT52800.4943959099974718No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48960.4584398438158375No Hit
CTATCATAGTGTTGGCAGTAATAACTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC48790.4568480388025881No Hit
GTATTAGTAGTTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGTCTA43530.40759571898087027No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42700.39982396509265244No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33450.31321104525408017No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32210.30160023221626076No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30980.29008305476745605No Hit
CACTATGATAGTGTTCCGTGGACATTCGGCCAAGGGACCAATGTGGAAATTAAACGAACTGTGGCTGCACCAT30500.28558854649475174No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29570.2768804367163871No Hit
ATCATAGTGTTGGCAGTAATAACTTGCAAAATCATCAGGCTGCAGGCTGC28280.2648014457334944No Hit
ACCTTAGAAAGTGGGGTCCCATCCAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC27710.25946421715965806No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27120.25393971740779236No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC25930.2427970823150463No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25080.2348380572487991No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24430.2287517439628454No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24320.227721752483684No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24230.22687903218255195No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAAGTTATTACTGCCAACACTATGATAGTGTT23470.21976272741743683No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23170.21695365974699665No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGCAGATACA23070.21601730385684992No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22170.20759010084552937No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21960.20562375347622125No Hit
GTGTATTACTGTGCGGGGTGTAGTGGTGGTATTTGCACACGCACAAATCC21940.20543648229819192No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20910.1957920166296806No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20290.1899866101107709No Hit
CTACTAATACTTTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACATATGCAGACAAGGTGGAAGGAGA19590.1834321188797438No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19490.18249576298959708No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18970.1776267123608341No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18920.17715853441576074No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18800.17603490734758467No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17800.16667134844611742No Hit
TTCTAAGGTAGACGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTT17560.16442409430976526No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17430.1632068316525745No Hit
GTGCAGCCACAGTTCGTTTAATTTCCACATTGGTCCCTTGGCCGAATGTC16840.15768233190070882No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT16650.15590325570943006No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTGGATGGGACCCCA16340.15300055244997518No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16100.15075329831362305No Hit
ATAGTGTTCCGTGGACATTCGGCCAAGGGACCAATGTGGAAATTAAACGA15740.1473824171090948No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15580.14588424768486008No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15260.14288790883639052No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14930.13979793439890634No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14920.13970429880989169No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCC14880.13932975645383297No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14760.1382061293856569No Hit
CCTTCCACCTTGTCTGCATATGTGGGAGACAGAGTCACCATCACTTGCCG14700.13764431585156886No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14570.13642705319437812No Hit
GAACACTATCATAGTGTTGGCAGTAATAACTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATT13880.1299661975523657No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATTTCCACATTGGTCC13840.129591655196307No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13270.12425442662247067No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13110.12275625719823591No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12930.1210708165959718No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12690.11882356245961966No Hit
TCTATAAGGCGTCTACCTTAGAAAGTGGGGTCCCATCCAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT12500.11704448626834088No Hit
GTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCCAACCAACTACTA12450.11657630832326751No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12330.11545268125509142No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12200.11423541859790069No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12100.11329906270775396No Hit
ATGATAGTGTTCCGTGGACATTCGGCCAAGGGACCAATGTGGAAATTAAA12090.11320542711873928No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12060.11292452035169527No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC11980.1121754356395779No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11950.11189452887253387No Hit
GTGTAGTGGTGGTATTTGCACACGCACAAATCCTTCTGACATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTC11910.11151998651647518No Hit
GTCAAAGTATTAGTAGTTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGG11880.11123907974943117No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11820.11067726621534313No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11610.108710918846035No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11510.10777456295588826No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11490.10758729177785893No Hit
CCCCTAAACTCCTGATCTATAAGGCGTCTACCTTAGAAAGTGGGGTCCCA11380.10655730029869752No Hit
GTATTACTGTGCGGGGTGTAGTGGTGGTATTTGCACACGCACAAATCCTT11210.1049654952854481No Hit
CTCCTGATCTATAAGGCGTCTACCTTAGAAAGTGGGGTCCCATCCAGGTT11160.10449731734037473No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAAGTTATTACTGCCAACACTATGATAGTGTTCCGTGGACATT11130.1042164105733307No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAAAGTATTAGTAGTTGGTTGGCCTGGTATCAGCAAAAA11080.10374823262825734No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11040.10337369027219866No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11030.10328005468318398No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11010.10309278350515465No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10950.1025309699710666No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGCAGATA10810.10122007172486118No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10730.1004709870127438No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10720.10037735142372914No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10720.10037735142372914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24650.038.184021
GCTTATC400.002747941834.102761
TATCAAC28850.032.2509082
ATCAACG29100.032.2080763
AACGCAG29550.031.7265136
TCAACGC29550.031.6022624
TCTGGAG8150.031.50757470
CAACGCA29900.031.349265
GGGACAA11400.031.18867570
CGCAGAG30500.030.6294068
AGAGTAC31400.029.96865511
GCAGAGT31700.029.6850389
CAGAGTA32050.029.25448410
ACGGGAT1254.6875357E-927.28732116
ACGCAGA35200.026.6365417
GCGTAGA26250.026.08627370
TACGGGG11850.025.90325715
AGTACGG30000.025.7971713
GAGTACG30200.025.62632812
TACGGGT7550.025.2971315
Produced by FastQC (version 0.11.5)