FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004685058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences728676
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24410.3349911346057782No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24000.3293644912142022No Hit
TAGTTATACTGCTGGCAGTAATAAGTTGCAAAATCATCAGCCTGCAGACTGCTTATGGTGAGAGCGAAATCCGTC20050.27515658536853144No Hit
GTCTGGGGCTGAGTTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGTA19340.2654128858367779No Hit
GTATAACTATTATCCATTTACGTTCGGCCCTGGGACCAAAGTGGAGATCAGACGAACTGTGGCTGCACCAT18940.25992347764987456No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGG16640.2283593805751802No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16640.2283593805751802No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT16090.22081144431818805No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14970.2054411013948586No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14920.2047549253714957No Hit
GGCTATTAGTAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAGGCCCCTACACTCCTGATCTACAAGGCGT14560.19981445800328268No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14240.19542293145375997No Hit
ATAGTAGACTGCCGAATCTTCAACTGCCAGACTGTTCAGTTCCATGTAGA14110.1936388737930164No Hit
CACCATAAGCAGTCTGCAGGCTGATGATTTTGCAACTTATTACTGCCAGCAGTATAACTATTATCCATTTACGTT13570.18622817274069683No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12940.17758235484632404No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12940.17758235484632404No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12890.1768961788229611No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12510.1716812410454029No Hit
GCTTATGGTGAGAGCGAAATCCGTCCCAGATGCACTGCCGCTGAACCTTGACGGGACCCCATTCTCCAAATTAGA12420.17044612420334962No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12420.17044612420334962No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGCTATTAGTAGTTGGTTGGCCTGGTATCAGCAGAAA12290.16866206654260604No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11980.16440777519775593No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11790.16180030630897682No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11740.1611141302856139No Hit
CCATAAGCAGTCTGCAGGCTGATGATTTTGCAACTTATTACTGCCAGCAG11220.15397789964263953No Hit
CTCCTGATCTACAAGGCGTCTAATTTGGAGAATGGGGTCCCGTCAAGGTT11100.15233107718656852No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10530.1445086705202312No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10390.14258737765481502No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT10210.1401171439707085No Hit
CCCCTACACTCCTGATCTACAAGGCGTCTAATTTGGAGAATGGGGTCCCG10170.13956820315201818No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10040.13778414549127457No Hit
GTGTAGGGGCCTTCCCTGGTTTCTGCTGATACCAGGCCAACCAACTACTA9990.13709796946791167No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9930.13627455823987616No Hit
CCTTCGACACTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG9830.13490220619315033No Hit
GTCTACTATTGTGCGAACGAAAGGGGTGGCCTCTACGACTACTGGGGTCA9730.13352985414642446No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9610.13188303169035348No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9470.12996173882493728No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9400.1290010923922292No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9370.12858938677821144No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9300.12762874034550337No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCGACA9240.12680532911746784No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9220.1265308587081227No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACTTTGGTCC9190.12611915309410493No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9150.1255702122754146No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8950.12282550818196292No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8880.12186486174925482No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACTTTGGTCCCAGGGCCGAACGTA8820.1210414505212193No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8760.12021803929318381No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8740.11994356888383863No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8500.11664992397169661No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8480.11637545356235145No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8390.11514033672029818No Hit
GAACTGAACAGTCTGGCAGTTGAAGATTCGGCAGTCTACTATTGTGCGAACGAAAGGGGTGGCCTCTACGACTA8340.11445416069693526No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8120.11143498619413841No Hit
GTCTGCAGGCTGATGATTTTGCAACTTATTACTGCCAGCAGTATAACTATTATCCATTTACGTTCGGCCCTGGGA8000.1097881637380674No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7970.10937645812404964No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7910.10855304689601415No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7860.10786687087265122No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC7780.10676898923527055No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7760.10649451882592538No Hit
CTATTATCCATTTACGTTCGGCCCTGGGACCAAAGTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCAT7680.10539663718854471No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7640.10484769636985437No Hit
GTTCAGTTCCATGTAGACCGTTGTCGCGACTGTGTCCATGGAAACGGTGA7470.10251469789042045No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7460.10237746268574785No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT7420.10182852186705751No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC7420.10182852186705751No Hit
CTAATAGCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA7360.10100511063902201No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)