FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences728676
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32770.4497197657120586No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24270.33306984174036197No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20910.2869588129703737No Hit
TAGTTATACTGCTGGCAGTAATAAGTTGCAAAATCATCAGCCTGCAGACTGCTTATGGTGAGAGCGAAATCCGTC20530.28174387519281546No Hit
GTCTGGGGCTGAGTTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGTA19500.2676086491115393No Hit
GTATAACTATTATCCATTTACGTTCGGCCCTGGGACCAAAGTGGAGATCAGACGAACTGTGGCTGCACCAT17790.2441414291125274No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15450.21202839121914266No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGG14530.19940275238926491No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14290.1961091074771229No Hit
GCTTATGGTGAGAGCGAAATCCGTCCCAGATGCACTGCCGCTGAACCTTGACGGGACCCCATTCTCCAAATTAGA14020.19240375695096312No Hit
ATAGTAGACTGCCGAATCTTCAACTGCCAGACTGTTCAGTTCCATGTAGA13890.19061969929021952No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13190.18101323496313862No Hit
CACCATAAGCAGTCTGCAGGCTGATGATTTTGCAACTTATTACTGCCAGCAGTATAACTATTATCCATTTACGTT13140.1803270589397757No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13000.1784057660743595No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12850.17634723800427077No Hit
GGCTATTAGTAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAGGCCCCTACACTCCTGATCTACAAGGCGT12840.17621000279959817No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11670.16015348385290581No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11290.1549385460753476No Hit
CCATAAGCAGTCTGCAGGCTGATGATTTTGCAACTTATTACTGCCAGCAG11110.1524683123912411No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10890.14944913788844424No Hit
CTCCTGATCTACAAGGCGTCTAATTTGGAGAATGGGGTCCCGTCAAGGTT10890.14944913788844424No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10800.14821402104639098No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGCTATTAGTAGTTGGTTGGCCTGGTATCAGCAGAAA10600.1454693169529393No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10530.1445086705202312No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10510.14423420011088606No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10500.14409696490621346No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10450.14341078888285053No Hit
GTCTACTATTGTGCGAACGAAAGGGGTGGCCTCTACGACTACTGGGGTCA10340.14190120163145212No Hit
CCCCTACACTCCTGATCTACAAGGCGTCTAATTTGGAGAATGGGGTCCCG10330.14176396642677952No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10090.1384703215146375No Hit
GTGTAGGGGCCTTCCCTGGTTTCTGCTGATACCAGGCCAACCAACTACTA9830.13490220619315033No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9700.13311814853240672No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9440.12955003321091954No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9390.1288638571875566No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9370.12858938677821144No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT9230.12666809391279527No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9160.12570744748008716No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9060.12433509543336133No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8870.12172762654458222No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACTTTGGTCCCAGGGCCGAACGTA8840.12131592093056448No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8740.11994356888383863No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8680.11912015765580314No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8620.11829674642776762No Hit
GTTCAGTTCCATGTAGACCGTTGTCGCGACTGTGTCCATGGAAACGGTGA8600.11802227601842244No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8460.11610098315300627No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8310.11404245508291752No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8210.11267010303619167No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8160.11198392701282875No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCGACA8160.11198392701282875No Hit
CTATTATCCATTTACGTTCGGCCCTGGGACCAAAGTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCAT8140.11170945660348358No Hit
GAACTGAACAGTCTGGCAGTTGAAGATTCGGCAGTCTACTATTGTGCGAACGAAAGGGGTGGCCTCTACGACTA8130.111572221398811No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8030.11019986935208514No Hit
CCTTCGACACTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG7980.10951369332872223No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7900.10841581169134155No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACTTTGGTCC7720.10594557800723504No Hit
GTCTGCAGGCTGATGATTTTGCAACTTATTACTGCCAGCAGTATAACTATTATCCATTTACGTTCGGCCCTGGGA7660.10512216677919953No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7490.1027891682997656No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7420.10182852186705751No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7410.10169128666238493No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)