FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences897892
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAACAGTATGGTACGTCACCTTGGACGTTCGGCCAAGGGA35460.39492500211606746No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31540.35126719026341696No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26120.29090358305898706No Hit
GTATGGTACGTCACCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT24360.2713021165128991No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC21790.24267952047685024No Hit
GTAATAAATGGCCGTGTCTGCGGCGGTCACAGAAATCAACTTCAGGGAGA21430.23867012959242315No Hit
GTCTATGACAGTGGGAGCACCAAGTACAATCCCTCCCTCAAGAGTCGAGT21310.2373336659642808No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19400.21606161988301487No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17360.19334173820459477No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17300.19267350639052358No Hit
GTCATAGACATAGCCAATCCACTCCAGTCCCCTCCCTGGGGGCTGCCGGA17150.19100292685534564No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16690.18587981628079991No Hit
GTACCATACTGTTGACAATAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC16370.1823159132724203No Hit
CAATAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16210.18053396176823047No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATT16070.17897475420206438No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15640.17418575953455426No Hit
GTCCCAGACCCACTGCCACTGAATTTGTCGGGGATGCCAGTGGCCCTGTT15090.16806030123890178No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14880.16572148988965266No Hit
ATCTCTGGTGGCTCCATCAATTCTTATTACTGGAGCTGGATCCGGCAGCC14790.16471914216854588No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14790.16471914216854588No Hit
CATCTATGGTGCATCCAACAGGGCCACTGGCATCCCCGACAAATTCAGTGGCAGTGGGTCTGGGACAGACTTCA14500.16148935506720186No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC14450.16093249522214254No Hit
GTGTTAGCAGCGCCTACTTAGCCTGGTACCACCAGAAACGTGGCCAGGCTCCCAGTCTCCTCATCTATGGTGCAT14390.16026426340807134No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14280.15903917174894086No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14150.15759133615178664No Hit
GTCTCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCACGCACCCT13940.15525252480253748No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCGCCTACTTAGCCTGGTACC13800.15369331723637142No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13760.15324782936032397No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13560.15102038998008668No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13510.1504635301350274No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13510.1504635301350274No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13390.149127066506885No Hit
GTACAATCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTACA13070.1455631634985054No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT13020.14500630365344608No Hit
GTCTTTCTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA12660.140996912769019No Hit
ATTTATTACTGTGCGAGACATTATCAGCAGTGGCTGGCAGTGGGGATCTT12470.1388808453577936No Hit
GTCTCCACGCACCCTGTCTTTCTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG12430.13843535748174612No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12340.13743300976063938No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12310.13709889385360377No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11780.131196179495975No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11780.131196179495975No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11610.12930285602277333No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC11460.1276322764875954No Hit
CCATAGATGAGGAGACTGGGAGCCTGGCCACGTTTCTGGTGGTACCAGGCTAAGTAGGCGCTGCTAACACTCTGA11260.1254048371073581No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10850.12083858637787173No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10850.12083858637787173No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCC10840.12072721440885986No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10810.12039309850182427No Hit
GTGTATTATTGTCAACAGTATGGTACGTCACCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA10560.11760879927652768No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10530.1172746833694921No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10500.11694056746245651No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10440.11627233564838534No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10440.11627233564838534No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10330.11504724398925484No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10310.11482450005123111No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGT10300.11471312808221923No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC10200.11359940839210061No Hit
CTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGC10160.11315392051605315No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10100.11248568870198199No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10010.1114833409808752No Hit
ATAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA9990.11126059704285148No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9920.11048099325976844No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC9780.10892178569360234No Hit
GAGTGTTAGCAGCGCCTACTTAGCCTGGTACCACCAGAAACGTGGCCAGG9740.1084762978175549No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAATTTGTCGGGGATGCCA9710.10814218191051932No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC9600.1069170902513888No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG9600.1069170902513888No Hit
GTACGTCACCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA9520.10602611449929389No Hit
GTCAGAGTGTTAGCAGCGCCTACTTAGCCTGGTACCACCAGAAACGTGGC9400.10468965087115155No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9290.10346455921202105No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9230.10279632739794986No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGTCTCAGAT9190.10235083952190242No Hit
TACTTAGCCTGGTACCACCAGAAACGTGGCCAGGCTCCCAGTCTCCTCATCTATGGTGCATCCAACAGGGCCA9150.10190535164585496No Hit
CTCCACGCACCCTGTCTTTCTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCG9090.10123711983178378No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9070.10101437589376006No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9040.10068025998672446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG2300.046.5308240
GCCGTCT2450.044.08217246
ATGCCGT2450.043.9296944
CGTATGC2500.042.87120441
CCGTCTT2550.042.4065547
GGTATCA9150.041.7420461
ATCTCGT2600.040.98393637
GACTAGG2500.040.94948226
CCCACGA2600.040.65752819
ATCTCCG2600.040.65310
TATACAC2700.040.410153
ACGAGAC2650.039.89262822
CGAGACT2700.039.15387323
AGACTAG2750.038.46340625
GCTTATC451.07223925E-437.8908461
TAGGCAT2750.037.27666529
CTTATAC2850.037.0931431
TTATACA2950.036.9855582
GTATCAA22900.036.4844131
GAGACTA2900.036.4617324
Produced by FastQC (version 0.11.5)