FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences848660
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAATATGGTAGTTCACCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA37430.44104824075601534No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29270.3448966606179153No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26500.31225696981123185No Hit
TCTATAGATCGTCCAGTAGGGCCACTGGCATCCCTGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT22640.266773501755709No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20910.24638842410388143No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18170.21410223175358803No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17650.2079749251761601No Hit
GTGTATTACTGTCAGCAATATGGTAGTTCACCTCCGCTCACTTTCGGCGG17380.20479343906864939No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16780.1977234699408479No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15970.18817901161831593No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15870.18700068343034904No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13890.16366978530860415No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA13440.15836730846275304No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13210.15565715363042915No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT13190.15542148799283576No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12570.1481158532274409No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12500.14729102349586407No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACAGACTGGAGCCTGAAGATT12460.14681969222067728No Hit
ATCTATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGCCTAAGTAGTCGCTGCTAACACT12090.14245987792519973No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12030.14175288101241956No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11760.1385713949049089No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11400.13432941342822802No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11030.12996959913275044No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC11020.12985176631395376No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC10710.12619894893125633No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10470.12337096128013574No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10430.12289963000494898No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10280.12113213772299862No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10120.11924681262225154No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG9990.11771498597789457No Hit
GTAATACACGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGAAGG9970.11747932034030117No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA9820.11571182805835081No Hit
GTGTTAGCAGCGACTACTTAGGCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATAGATCGT9660.11382650295760374No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9510.11205901067565338No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9480.1117055122192633No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTTCTGCAGGGCCAGTCAGAGTGTTAGCAG9450.11135201376287324No Hit
CATCTATAGATCGTCCAGTAGGGCCACTGGCATCCCTGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA9210.10852402611175263No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8900.10487120872905523No Hit
GACTTCACTCTCACCATCAACAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAATATGGTAGTTCA8700.10251455235312139No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCAGGGATGCCAGTGGCCCTACT8620.10157188980274787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC2600.051.90868827
CGTATGC2150.051.45334641
AAACGTA400.008835765549.40497670
TACGCTG2750.049.18486829
TCGTATG2050.048.79782540
CTACGCT2850.047.4085328
CGCGTAT306.377221E-445.9018239
GTATGCC2700.044.9220642
ATCTCGT2300.044.6374637
GAGACTA3100.044.52997624
GGTATCA11350.043.2792781
TTATACA3050.043.0065162
TGCCGTC2600.042.9327545
TATACAC3400.041.5470163
CACGAGA3250.041.41923521
CTTATAC3250.041.409471
CCGTCTT2650.041.00934247
ATGCCGT2900.040.79991544
ACGAGAC3500.040.3861322
CATCTCG2800.037.8229436
Produced by FastQC (version 0.11.5)