FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484225
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT44430.9175486602302649No Hit
GTATTATTGTCAACAGTATGGTACGTCACCTTGGACGTTCGGCCAAGGGA24060.4968764520625742No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19790.40869430533326445No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16350.3376529505911508No Hit
GTATGGTACGTCACCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT15560.321338220868398No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC13840.2858175434973411No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13040.26929629820847745No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11330.2339821364035314No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10670.2203521090402189No Hit
GTCTATGACAGTGGGAGCACCAAGTACAACCCCTCCCTCAAGAGTCGAGT10620.2193195312096649No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATT10580.21849346894522176No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10390.21456967318911666No Hit
GTAATAAATGGCCGTGTCTGCGGCGGTCACAGAATTCAACTTCAGGGAGA10250.21167845526356546No Hit
CAATAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA9850.20341783261913368No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9610.19846145903247456No Hit
CATCTATGGTGCATCCAACAGGGCCACTGGCATCCCCGACAAATTCAGTGGCAGTGGGTCTGGGACAGACTTCA9480.19577675667303424No Hit
GTGTTAGCAGCGCCTACTTAGCCTGGTACCACCAGAAACGTGGCCAGGCTCCCAGTCTCCTCATCTATGGTGCAT9390.19391811657803706No Hit
GTACCATACTGTTGACAATAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC9340.1928855387474831No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9130.1885487118591564No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCGCCTACTTAGCCTGGTACC9080.18751613402860243No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC9070.1873096184624916No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8920.1842118849708297No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8890.1835923382724973No Hit
GTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA8820.18214672930972173No Hit
GTCTCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCACGCACCCT8820.18214672930972173No Hit
GTCCCAGACCCACTGCCACTGAATTTGTCGGGGATGCCAGTGGCCCTGTT7960.1643863906241933No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7790.16087562600030977No Hit
GTCTCCACGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG7780.160669110434199No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7720.1594300170375342No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7610.15715834581031546No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7480.1544736434508751No Hit
ATCTCTGGTGGCTCCATCAATTCTTATTACTGGAGCTGGATCCGGCAGCC7370.15220197222365636No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7190.14848469203366205No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7150.14765862976921887No Hit
GTGTATTATTGTCAACAGTATGGTACGTCACCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA7020.14497392740977852No Hit
GTCATAGACATAGCCAATCCACTCCAGTCCCCTCCCTGGGGGCTGCCGGA7000.14456089627755692No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGT6910.14270225618255974No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC6870.14187619391811657No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6830.1410501316536734No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6810.1406371005214518No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCC6760.13960452269089782No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC6650.13733285146367907No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG6650.13733285146367907No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6550.13526769580257111No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATTTTGCAGTGTATTATTGTCAACAGTATGGTACGTCA6490.13402860240590636No Hit
GAGTGTTAGCAGCGCCTACTTAGCCTGGTACCACCAGAAACGTGGCCAGG6460.13340905570757394No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC6420.13258299344313076No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6410.13237647787701998No Hit
TACTTAGCCTGGTACCACCAGAAACGTGGCCAGGCTCCCAGTCTCCTCATCTATGGTGCATCCAACAGGGCCA6380.13175693117868761No Hit
GTACGTCACCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA6300.13010480664980123No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6280.12969177551757963No Hit
GTCAGAGTGTTAGCAGCGCCTACTTAGCCTGGTACCACCAGAAACGTGGC6210.12824616655480406No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6160.1272135887242501No Hit
ATTTATTACTGTGCGAGACATTATCAGCAGTGGCTGGCAGTAGGGATCTT6090.12576797976147452No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6050.12494191749703133No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5970.12328979296814498No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAATTTGTCGGGGATGCCA5890.12163766843925862No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGTCTCAGAT5860.12101812174092623No Hit
ATAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA5760.11895296607981826No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5620.11606174815426712No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG5610.11585523258815633No Hit
CCATAGATGAGGAGACTGGGAGCCTGGCCACGTTTCTGGTGGTACCAGGCTAAGTAGGCGCTGCTAACACTCTGA5580.11523568588982394No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC5560.11482265475760237No Hit
GTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTACA5520.11399659249315917No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG5510.11379007692704837No Hit
CTCCACGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCG5340.11027931230316486No Hit
TCTATGGTGCATCCAACAGGGCCACTGGCATCCCCGACAAATTCAGTGGCAGTGGGTCTGGGACAGACTTCACT5240.10821415664205689No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA5170.10676854767928132No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT5150.10635551654705974No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5120.10573596984872735No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5050.10429036088595177No Hit
CTGCTACTCTGGCTCCCAGTCTCAGATACCACCGGAGAAATTGTGTTGAC5020.10367081418761939No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC4990.103051267489287No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC4980.1028447519231762No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG4970.10263823635706543No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC4940.10201868965873302No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAT150.00236912568.8937139
CGCGTAT301.142289E-768.8937139
TATCGTA150.00239707168.6902938
ATATCGT150.00241617468.5529437
ATCGCGT354.3728505E-968.5529337
AATCGCG401.27856765E-859.85154336
TCGCGTA453.1324817E-853.4257838
AATATCG200.007614068751.30132336
CCGATGC200.00804983250.58423227
CTAGTGG200.00813252150.453633
CTCTTAT1500.049.363021
AAGGGGG7850.048.6733269
AAAGGGG6950.048.59485668
TGAATCG507.649578E-847.7009134
ATGCCGT7350.046.95444
GCCGTCT6900.046.66451646
GCGTATG451.8516967E-646.02649740
CGTCTTC6850.045.68344548
TTATACA7450.045.600152
CGTATGC7750.045.5194541
Produced by FastQC (version 0.11.5)