FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484225
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82301.6996231090918479No Hit
GTATTATTGTCAACAGTATGGTACGTCACCTTGGACGTTCGGCCAAGGGA23080.47663792658371623No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20610.42562858175434976No Hit
GTATGGTACGTCACCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT14460.29862150859621045No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13820.2854045123651195No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC12640.2610356755640456No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11520.2379059321596365No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10650.21993907790799733No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10310.21291754866023027No Hit
GTAATAAATGGCCGTGTCTGCGGCGGTCACAGAATTCAACTTCAGGGAGA10160.20981981516856835No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATT10140.20940678403634674No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10110.20878723733801433No Hit
GTCTATGACAGTGGGAGCACCAAGTACAACCCCTCCCTCAAGAGTCGAGT9720.20073313025969333No Hit
GTACCATACTGTTGACAATAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC9620.19866797459858537No Hit
CAATAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA9610.19846145903247456No Hit
GTGTTAGCAGCGCCTACTTAGCCTGGTACCACCAGAAACGTGGCCAGGCTCCCAGTCTCCTCATCTATGGTGCAT9520.19660281893747739No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8860.1829727915741649No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCGCCTACTTAGCCTGGTACC8710.17987505808250298No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8620.1780164179875058No Hit
CATCTATGGTGCATCCAACAGGGCCACTGGCATCCCCGACAAATTCAGTGGCAGTGGGTCTGGGACAGACTTCA8610.177809902421395No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8440.1742991377975115No Hit
GTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA7790.16087562600030977No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7750.1600495637358666No Hit
GTCCCAGACCCACTGCCACTGAATTTGTCGGGGATGCCAGTGGCCCTGTT7680.15860395477309103No Hit
GTGTATTATTGTCAACAGTATGGTACGTCACCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA7570.15633228354587225No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7430.15344106562032112No Hit
ATCTCTGGTGGCTCCATCAATTCTTATTACTGGAGCTGGATCCGGCAGCC7360.15199545665754557No Hit
GTCTCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCACGCACCCT7360.15199545665754557No Hit
ATTTATTACTGTGCGAGACATTATCAGCAGTGGCTGGCAGTAGGGATCTT7100.14662605193866488No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7010.14476741184366773No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7010.14476741184366773No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC6970.14394134957922455No Hit
GTCTCCACGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG6940.14332180288089214No Hit
GTCATAGACATAGCCAATCCACTCCAGTCCCCTCCCTGGGGGCTGCCGGA6800.140430584955341No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6790.1402240693892302No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6740.13919149155867624No Hit
GAGTGTTAGCAGCGCCTACTTAGCCTGGTACCACCAGAAACGTGGCCAGG6710.13857194486034385No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC6670.13774588259590068No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATTTTGCAGTGTATTATTGTCAACAGTATGGTACGTCA6510.13444163353812794No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6500.13423511797201715No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGT6360.131343900046466No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6310.13031132221591202No Hit
ATAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA6220.12845268212091487No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6220.12845268212091487No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6170.12742010429036088No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC6150.1270070731581393No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC6100.1259744953275853No Hit
GTCAGAGTGTTAGCAGCGCCTACTTAGCCTGGTACCACCAGAAACGTGGC6090.12576797976147452No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCC6010.12411585523258817No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA5780.11936599721203986No Hit
CCATAGATGAGGAGACTGGGAGCCTGGCCACGTTTCTGGTGGTACCAGGCTAAGTAGGCGCTGCTAACACTCTGA5710.11792038824926429No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT5700.11771387268315349No Hit
GTACGTCACCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA5650.11668129485259951No Hit
TACTTAGCCTGGTACCACCAGAAACGTGGCCAGGCTCCCAGTCTCCTCATCTATGGTGCATCCAACAGGGCCA5630.11626826372037793No Hit
CTCCACGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCG5620.11606174815426712No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAATTTGTCGGGGATGCCA5450.1125509835303836No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG5440.1123444679642728No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5400.11151840569982963No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5330.11007279673705406No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5280.10904021890650008No Hit
GTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTACA5270.10883370334038929No Hit
CTGCTACTCTGGCTCCCAGTCTCAGATACCACCGGAGAAATTGTGTTGAC5260.10862718777427849No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG5170.10676854767928132No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5150.10635551654705974No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG5120.10573596984872735No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5120.10573596984872735No Hit
TCTATGGTGCATCCAACAGGGCCACTGGCATCCCCGACAAATTCAGTGGCAGTGGGTCTGGGACAGACTTCACT5070.10470339201817337No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGTCTCAGAT5050.10429036088595177No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA5050.10429036088595177No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT5030.10387732975373018No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5020.10367081418761939No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA4980.1028447519231762No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGAT158.628952E-488.8516469
TATAATG252.9317426E-453.729372
AGATAGC200.00814184750.4389922
TAAGACA307.205379E-444.774474
GTGCTAG705.7298166E-1043.206621
ACAGGCC504.597856E-640.3261761
GACAGGT957.2759576E-1238.9112241
GTACAGC350.001538215738.378126
GTATCAA12100.033.8828771
GAATACG808.976349E-833.5808569
GGTATCA5450.033.296841
CTAGAGA851.5284604E-731.605514
ATACGTA851.5284604E-731.6055111
AGAATAC851.5284604E-731.605518
CACTTTA652.7886608E-530.9977135
TAGTCAC450.00501389130.15795332
AATAGTC450.005091109330.06385630
ACGGGTC450.005272282329.8496485
GCTAGAG902.52272E-729.8496483
TACGTAC902.52272E-729.84964812
Produced by FastQC (version 0.11.5)