FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589103
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24380.4138495305574747No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16390.27821959827059106No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15960.2709203653690441No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15580.264469880479305No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15570.26430013087694343No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCCTGGTCCCTCCGCCGAATACCACGGTCCTGATGGTTGTATATGAACTG14840.25190840990454977No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13480.2288224639833781No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12900.21897698704640783No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12680.21524249579445362No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCCGGGCCCAGTC11880.20166252760552908No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11190.1899498050425817No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11010.18689431220007366No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG10410.17670933605838027No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10370.17603033764893405No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCATTGGAACCAGCAGTGACGTT10230.17365384321587227No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9460.16058312383403242No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG9180.15583013496790885No Hit
GTCCTGATGGTTGTATATGAACTGCAGAAATAGTCAGCCTCGTCCTCCGC9110.15464188775137794No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT8820.1497191492828928No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCCTGGTCCCTC8670.14717290524746945No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8540.14496616041676924No Hit
CATCAGGACCGTGGTATTCGGCGGAGGGACCAGGCTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCA8390.14241991638134588No Hit
GGGCACAGGGTCCCGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC8090.13732742831049918No Hit
GTCCTTATAGTGCGACAACAGAATATGCCACGTCTGTGAAAGACAGATTC7900.1341021858656296No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT7870.13359293705854494No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7810.1325744394443756No Hit
TCTCTGGGCTCCAGGCGGAGGACGAGGCTGACTATTTCTGCAGTTCATAT7800.13240468984201406No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG7780.13206519063729094No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTGTGGTGGTCTCCA7670.13019794501131382No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7600.1290096977947829No Hit
CCACCACACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAT7510.1274819513735289No Hit
GTCGATCACCATCTCCTGCATTGGAACCAGCAGTGACGTTGGTGCTTATAATTATGTCTCCTGGTACCAACAACA7450.12646345375935958No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7330.12442645853102088No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC7270.12340796091685155No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG6740.11441123199168905No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA6450.10948849352320392No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG6390.1084699959090346No Hit
CTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGA6320.1072817486925037No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT6270.10643300068069592No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG5990.10168001181457233No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT5960.10117076300748765No Hit
GTTCATATACAACCATCAGGACCGTGGTATTCGGCGGAGGGACCAGGCTGACCGTCCTCGGTCAGCCCAAGGCTG5910.10032201499567987No Hit
CTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGG5900.10015226539331831No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC5900.10015226539331831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7450.044.5514341
TGGTATC2550.041.5622442
GTATCAA20400.039.9202271
AATGGTA2450.037.49391670
TATCAAC23500.034.333782
ATCAACG25300.031.891063
AACGCAG25650.031.5945456
TCAACGC25650.031.4558964
CAACGCA25900.031.1549135
CGCAGAG26300.030.8136968
GGTACGC450.004826624430.3947568
ACGCAGA26650.030.2807047
AGAGTAC26950.029.81674811
GCAGAGT27200.029.7941259
CAGAGTA27400.029.4518510
AACGGAG703.9627063E-529.444233
GTAAACA658.024364E-426.882849
ATGTGAT2007.755116E-726.24573970
GAGTACT9700.026.0862212
AAACGGA809.849564E-525.75928532
Produced by FastQC (version 0.11.5)