FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1506737
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTATACTACCCCTCTCACTTTCGGCGGAGGGACCAGGGTGGAAATGAATCGAACTGTGGCTGCACCAT53160.35281538848518357No Hit
GTAGTAGAGGGCCGTGTCGTCGGCTCTCAGGTTGTTCATTTGCAGATATA49530.32872359277033747No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45980.30516274572138335No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42870.2845221163348348No Hit
CTCTACTACTGTGCGAAACATCGCGGAGACTCTATCAGATACGACCTTTACTTTGACTTCTGGGGCCAGGGGACC33030.21921543043012817No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT30650.2034197076198434No Hit
GTATAACTCTGCTGACAGTAGTAGATTCCAAAATCTTCACGGTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC30610.20315423328689744No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28610.18988051663959934No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28350.1881549334754506No Hit
ATACTACCCCTCTCACTTTCGGCGGAGGGACCAGGGTGGAAATGAATCGA27080.1797261234044163No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG27010.17926154332176086No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23960.15901912543463126No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23780.15782449093637443No Hit
GGGTAGTATAACTCTGCTGACAGTAGTAGATTCCAAAATCTTCACGGTGCAGACTGCTGATGGTGAGAGTGAAAT23460.15570069627280672No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23410.1553688533566243No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23200.15397511310865797No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22420.1487983636162117No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT22270.14780283486766438No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21880.14521446012144124No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21740.14428529995613038No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20240.1343300124706568No Hit
CTACTACTGTGCGAAACATCGCGGAGACTCTATCAGATACGACCTTTACT20140.1336663266382919No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19890.13200711205737964No Hit
GTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACCGCCACTGAACCTGGACGGGACCCCAGTCT19870.13187437489090664No Hit
GTTTTGTACAGTGGTATCTGCAAAAACCGGGAGAAGCCCCTAAACTCCTGATCTATGCTTCATTCATTTTAGAGA19530.12961784306086596No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19340.12835683997937264No Hit
CTCCTGTGGAGGGTCTGGATTCATTTTCAGCGACTATGCCATCAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG19290.1280249970631902No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19120.12689673114816985No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18810.12483930506783864No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18710.12417561923547375No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT18550.12311372190368988No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18390.12205182457190604No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC17920.11893250115979098No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17750.11780423524477064No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT17570.11660960074651383No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC17280.1146849118326556No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17190.11408759458352719No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17130.11368938308410825No Hit
GTCTCCATCCTCGGTGTCTGCATCTGTGGGGGAGAGAGTCACCATCACTT16910.11222927425290545No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16890.11209653708643247No Hit
GTGCAGCCACAGTTCGATTCATTTCCACCCTGGTCCCTCCGCCGAAAGTG16640.1104373225055202No Hit
CTCCTGATCTATGCTTCATTCATTTTAGAGACTGGGGTCCCGTCCAGGTT16640.1104373225055202No Hit
CTACTACTGTCAGCAGAGTTATACTACCCCTCTCACTTTCGGCGGAGGGA16580.11003911100610127No Hit
CAGTAGTAGAGGGCCGTGTCGTCGGCTCTCAGGTTGTTCATTTGCAGATA16530.1097072680899188No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15950.10585789026220237No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15900.10552604734601992No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC15740.10446415001423608No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15520.10300404118303327No Hit
TTTCCACCCTGGTCCCTCCGCCGAAAGTGAGAGGGGTAGTATAACTCTGCTGACAGTAGTAGATTCCAAAATCTT15200.10088024651946556No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATTCG304.897221E-448.43011561
GGTATCA12950.043.3826451
TCGTATG5150.041.47629540
CGTATGC5300.040.36791241
ATCTCGT4900.038.46534737
TCTGGAG16050.038.16137770
CTTATAC5650.037.138211
ACGAGAC5750.037.09076322
GTATCAA33450.036.929591
GTATGCC5900.036.91129742
TATACAC5800.036.7514883
ATGCCGT6050.035.56222544
TCTCCGA5900.035.55747211
CACGAGA6000.035.5417721
CGAGACT6000.034.9857523
ATCTCCG6000.034.96484810
CTAGGCA6050.034.7693528
GAGACTA6150.034.6887224
TAGGCAT6100.034.50617229
TTATACA6100.034.3893742
Produced by FastQC (version 0.11.5)