FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1506737
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62430.4143390651454103No Hit
GAGTTATACTACCCCTCTCACTTTCGGCGGAGGGACCAGGGTGGAAATGAATCGAACTGTGGCTGCACCAT50570.33562592542693254No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42420.28153553008919274No Hit
GTAGTAGAGGGCCGTGTCGTCGGCTCTCAGGTTGTTCATTTGCAGATATA41070.2725757713522665No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39580.26268685245002943No Hit
CTCTACTACTGTGCGAAACATCGCGGAGACTCTATCAGATACGACCTTTACTTTGACTTCTGGGGCCAGGGGACC32170.21350773227178996No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28980.1923361542193495No Hit
GTATAACTCTGCTGACAGTAGTAGATTCCAAAATCTTCACGGTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC28750.1908096768049102No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG28120.1866284560610113No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25410.16864257000392238No Hit
ATACTACCCCTCTCACTTTCGGCGGAGGGACCAGGGTGGAAATGAATCGA24720.16406313776060455No Hit
GGGTAGTATAACTCTGCTGACAGTAGTAGATTCCAAAATCTTCACGGTGCAGACTGCTGATGGTGAGAGTGAAAT23030.15284684719363764No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23000.15264774144392818No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21760.14441803712260334No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21630.14355524554052898No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21090.13997134204575848No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21040.13963949912957604No Hit
CTACTACTGTGCGAAACATCGCGGAGACTCTATCAGATACGACCTTTACT20640.1369847558001164No Hit
CTCCTGTGGAGGGTCTGGATTCATTTTCAGCGACTATGCCATCAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG20480.13592285846833257No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20070.13320174655563646No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19710.1308124775591228No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT19630.13028152889323089No Hit
GTTTTGTACAGTGGTATCTGCAAAAACCGGGAGAAGCCCCTAAACTCCTGATCTATGCTTCATTCATTTTAGAGA19390.1286886828955551No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19020.12623304531580493No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC19010.12616667673256846No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18810.12483930506783864No Hit
GTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACCGCCACTGAACCTGGACGGGACCCCAGTCT18670.12391014490252777No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18270.12125540157306815No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18110.1201935042412843No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG17690.11740602374535171No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17620.11694144366269628No Hit
CTCCTGATCTATGCTTCATTCATTTTAGAGACTGGGGTCCCGTCCAGGTT17500.11614502066385839No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17230.11435306891647314No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16980.11269385433556088No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16780.11136648267083106No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16720.11096827117141213No Hit
GGGTGGAAATGAATCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG16480.10937542517373636No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC16370.10864537075813496No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT15910.10559241592925639No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15870.10532694159631044No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15710.1042650442645266No Hit
GTGCAGCCACAGTTCGATTCATTTCCACCCTGGTCCCTCCGCCGAAAGTG15690.10413230709805361No Hit
GTCTCCATCCTCGGTGTCTGCATCTGTGGGGGAGAGAGTCACCATCACTT15520.10300404118303327No Hit
CTACTACTGTCAGCAGAGTTATACTACCCCTCTCACTTTCGGCGGAGGGA15380.10207488101772241No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15220.10101298368593856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)