FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences796948
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAATATTGCTGGCAGAAATAAGTTGCGAAGTCCTCAGGCTGCAGGCTGTTGATGGTGAGCGTGAAATCTGTC29040.3643901484162078No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27040.3392944081671577No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25440.3192178159679176No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19320.2424248508058242No Hit
ATATTACTCTTTCCCGTATACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCAT18080.22686549185141314No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC16970.2129373560131903No Hit
GATCTATACTTCCTTCACTTTACAAAGTGGAGTCCCATCAAGATTTAGTG16570.20791820796338029No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16280.20427932562726803No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15930.19988757108368427No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT15320.19223337030772397No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14440.1811912445981419No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13650.1712784271997671No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13580.17040007629105036No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC13470.1690198105773526No Hit
GTCTGGGACTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCCTCTGGATACGTCTTCAGGA13280.16663571525369283No Hit
GTATAGATCAAGGACTTAGGGGCTTTCCCAGGTTTCTGGTGAAACCAGGC12450.15622098305033705No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12310.15446428123290354No Hit
GTTGATGGTGAGCGTGAAATCTGTCCCAGATCCACTGCCACTAAATCTTG12290.15421332383041303No Hit
AGTCAGGGCATTGGCAATTCTTTAGCCTGGTTTCACCAGAAACCTGGGAA12280.15408784512916776No Hit
GTCTATATGGAGTTGAACAGTCTGACATCTGAAGACACGGCTGTTTATTA11950.1499470479880745No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11660.14630816565196222No Hit
GTCCTCAGGCTGCAGGCTGTTGATGGTGAGCGTGAAATCTGTCCCAGATCCACTGCCACTAAATCTTGATGGGA11610.145680772145736No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11370.14266928331584997No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT11000.1380265713697757No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10910.13689726305856845No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10850.13614439085109692No Hit
GTCTCCATCCTCGCTGTCTGCATCTGTAGGAGACAGAATTACCATCACTT10820.1357679547473612No Hit
CCATCAACAGCCTGCAGCCTGAGGACTTCGCAACTTATTTCTGCCAGCAA10760.1350150825398897No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10560.13250550851498466No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10520.13200359371000367No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCT10410.1306233279963059No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10400.13049784929506067No Hit
GTCTGCATCTGTAGGAGACAGAATTACCATCACTTGTCGGGCGAGTCAGG9890.12409843553155288No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9610.12058503189668586No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9560.1199576383904596No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9510.11933024488423336No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9420.11820093657302609No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT9420.11820093657302609No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT9340.11719710696306408No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9170.11506396904189484No Hit
GTAATAAACAGCCGTGTCTTCAGATGTCAGACTGTTCAACTCCATATAGA9060.11368370332819706No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8930.11205248021200881No Hit
GTTTCCCAGGTGCCAGATGTGACATTCAGATGACCCAGTCTCCATCCTCG8900.11167604410827305No Hit
CACCATCAACAGCCTGCAGCCTGAGGACTTCGCAACTTATTTCTGCCAGC8880.11142508670578255No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACATTCAGATGACCCAGTCTCCATCCTCG8880.11142508670578255No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8870.11129960800453731No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCAGCTTGGTCC8740.10966838488834907No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC8720.10941742748585856No Hit
GAATTACCATCACTTGTCGGGCGAGTCAGGGCATTGGCAATTCTTTAGCCTGGTTTCACCAGAAACCTGGGAAAG8440.10590402385099154No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8380.10515115164352004No Hit
GTGGAGTCCCATCAAGATTTAGTGGCAGTGGATCTGGGACAGATTTCACG8330.10452375813729377No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC8310.10427280073480327No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8280.10389636463106752No Hit
GTGCAGCCACAGTTCCTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTA8240.10339444982608653No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC8190.10276705631986026No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG8140.10213966281363401No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8080.10138679060616251No Hit
CTGTTGATGGTGAGCGTGAAATCTGTCCCAGATCCACTGCCACTAAATCT8040.10088487580118151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACA2450.048.30284523
CGTATGC2650.046.9144641
TCGTATG2650.046.8513440
ACGAGAC2700.043.8193522
ATCTCGT2500.042.54139337
CTTATAC3000.041.6726651
GGTATCA9900.041.638531
ATCTCCG2850.041.4818110
TCTCCGA2900.040.7666111
ATGCCGT2900.040.76036544
TTATACA3150.040.748122
CACGAGA3000.040.55910521
TATACAC3100.040.3157353
GTATGCC2950.039.89175442
GAGTTAT350.001492869238.6155931
CCGAGAC350.001495173138.603483
CCGTCTT3100.038.37102547
GTATCAA24100.037.153491
CGGCAAA851.2375144E-436.9736370
GGATCTC2900.036.55016735
Produced by FastQC (version 0.11.5)