FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences796948
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31370.39362668580635124No Hit
GAGTAATATTGCTGGCAGAAATAAGTTGCGAAGTCCTCAGGCTGCAGGCTGTTGATGGTGAGCGTGAAATCTGTC27990.35121488478545654No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24560.3081756902583355No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22100.2773079297520039No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19010.23853501106722144No Hit
ATATTACTCTTTCCCGTATACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAGGAACTGTGGCTGCACCAT18200.22837123626635614No Hit
GATCTATACTTCCTTCACTTTACAAAGTGGAGTCCCATCAAGATTTAGTG15990.20064044329115577No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC15150.19010023238655469No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14530.18232055290934918No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13570.1702745975898051No Hit
GTCTGGGACTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCCTCTGGATACGTCTTCAGGA12870.16149108850263755No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12410.15571906824535603No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12370.15521715344037504No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12050.15120183500052703No Hit
GTATAGATCAAGGACTTAGGGGCTTTCCCAGGTTTCTGGTGAAACCAGGC11900.14931965448184825No Hit
GTCCTCAGGCTGCAGGCTGTTGATGGTGAGCGTGAAATCTGTCCCAGATCCACTGCCACTAAATCTTGATGGGA11710.14693555915818848No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11570.14517885734075497No Hit
GTCTATATGGAGTTGAACAGTCTGACATCTGAAGACACGGCTGTTTATTA11560.14505337863950973No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11540.14480242123701922No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC11470.14392407032830248No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11330.14216736851086897No Hit
GTCTCCATCCTCGCTGTCTGCATCTGTAGGAGACAGAATTACCATCACTT11170.14015970929094496No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11130.13965779448596394No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11080.1390304009797377No Hit
GTTGATGGTGAGCGTGAAATCTGTCCCAGATCCACTGCCACTAAATCTTG10920.1370227417598137No Hit
AGTCAGGGCATTGGCAATTCTTTAGCCTGGTTTCACCAGAAACCTGGGAA10750.13488960383864443No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10630.1333838594237014No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10530.1321290724112489No Hit
CCATCAACAGCCTGCAGCCTGAGGACTTCGCAACTTATTTCTGCCAGCAA10360.12999593449007965No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10270.1288666261788724No Hit
GTCTGCATCTGTAGGAGACAGAATTACCATCACTTGTCGGGCGAGTCAGG10040.12598061605023164No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCT9950.12485130773902439No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9920.12447487163528863No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT9460.1187028513780071No Hit
GTAATAAACAGCCGTGTCTTCAGATGTCAGACTGTTCAACTCCATATAGA9040.11343274592570657No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8980.11267987371823505No Hit
CACCATCAACAGCCTGCAGCCTGAGGACTTCGCAACTTATTTCTGCCAGC8850.11104865060204681No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA8810.11054673579706581No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT8750.1097938635895943No Hit
GTTTCCCAGGTGCCAGATGTGACATTCAGATGACCCAGTCTCCATCCTCG8640.10841359787589655No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACATTCAGATGACCCAGTCTCCATCCTCG8570.10753524696717978No Hit
CCATATAGACTGTGCTCTCGGAGGTGTCCCTGGTGATGGTGACTCTGCCCTGGAAGACATGTGAATATTGCGTA8540.10715881086344405No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC8510.10678237475970828No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8350.10477471553978428No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC8340.10464923683853904No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG8330.10452375813729377No Hit
GAATTACCATCACTTGTCGGGCGAGTCAGGGCATTGGCAATTCTTTAGCCTGGTTTCACCAGAAACCTGGGAAAG8310.10427280073480327No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCAGCTTGGTCC8250.10351992852733177No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8220.10314349242359602No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8110.10176322670989825No Hit
CTGTTGATGGTGAGCGTGAAATCTGTCCCAGATCCACTGCCACTAAATCT8010.10050843969744575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10900.043.9030651
CGGTACC651.0280928E-842.95016545
TTAACGG702.3810571E-839.0517335
GTATCAA26900.034.8277551
GTGGTAT2950.034.271431
TAACGGC807.585368E-834.23567636
TAACCGT859.292853E-833.45347653
TGGTATC3200.032.6163752
CCCTAAC901.6255035E-731.38908850
TATCAAC30050.030.8114552
CACGAAC902.448851E-729.9557671
ATCAACG31150.029.7196793
ACCGTGC1102.5929694E-829.246455
TCAACGC31600.029.1899264
AACGCAG31600.029.0870446
CAACGCA31750.028.9478075
ACGCAGA32950.027.8953117
GCGGTAC1004.6384594E-727.8469744
CGCAGAG33450.027.78038
AGTTTAT2800.027.6562822
Produced by FastQC (version 0.11.5)