FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1075767
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTATTCCGTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT58150.5405445602997675No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCGAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC52250.4856999703467387No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA41040.38149524943598384No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38240.35546730844132607No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36710.34124489782638806No Hit
GAGTATTGGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGTCCCCAAACTCCTAATCTATAAGGCGT31280.2907692836831767No Hit
AGTTTAGAGAGTGGGGTCCTATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC29560.27478069135788696No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28140.26158080699631053No Hit
CTCTAAACTAGACGCCTTATAGATTAGGAGTTTGGGGACTTTCCCTGGTT26420.24559221467102074No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25750.23936410021872767No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC25100.23332189963068212No Hit
GTATATTACTGTGCGAATCATAGAAAGCAGTGGCTTAACACTGATGCTTT24530.22802335449962677No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT24450.22727969904263656No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22700.21101223592097546No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22630.210361537396109No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG21950.20404046601169212No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATGACTTGCCG21860.20320385362257812No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21830.20292498282620677No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATGACTTGCCGGGCCAGTCAGA21190.19697573917028502No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20860.19390816041020034No Hit
GTGTTAAGCCACTGCTTTCTATGATTCGCACAGTAATATACGGCCGTGTC20770.19307154802108634No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20680.19223493563197236No Hit
GTAGGAGACAGAGTCACCATGACTTGCCGGGCCAGTCAGAGTATTGGTAGCTGGTTGGCCTGGTATCAGCAGAAA20680.19223493563197236No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20320.18888848607551634No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20250.1882377875506499No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20100.18684343356879324No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17790.1653703822482006No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17790.1653703822482006No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17790.1653703822482006No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17660.1641619421305915No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT17620.16379011440209634No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT17550.1631394158772299No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTCGCAACTTA16800.15616764596794658No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16730.15551694744308014No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16150.15012544537990102No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG15780.14668603889132126No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15700.14594238343433105No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC15680.14575646957008348No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT15430.14343254626698904No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15310.14231706308150371No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC15280.14203819228513237No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15000.13943539818566658No Hit
CTCCTAATCTATAAGGCGTCTAGTTTAGAGAGTGGGGTCCTATCAAGGTT14850.13804104420380994No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14760.13720443181469594No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14160.13162701588726927No Hit
GTATTGGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGTCCCC14090.1309763173624028No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13980.12995379110904126No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG13950.12967492031266992No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATGACTTGCCGGGCCAGTCAGAGTATTGGTAG13850.12874535099143214No Hit
CTACGTAGACTCCGTGAAGGGCCGCTTCACCATCTCCAGAGACAATCCCAAGAACACGCTGTATCTGCAAATGAA13770.12800169553444193No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13700.1273509970095755No Hit
CAGTATAATAGTTATTCCGTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT13680.12716508314532793No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13540.12586368609559503No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13400.12456228904586215No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13320.12381863358887195No Hit
ACTTATTACTGCCAACAGTATAATAGTTATTCCGTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA13310.12372567665674815No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13290.1235397627925006No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG12980.1206580978966635No Hit
GATTAGGAGTTTGGGGACTTTCCCTGGTTTCTGCTGATACCAGGCCAACC12870.11963557164330194No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC12810.11907783005055929No Hit
CCAATACTCTGACTGGCCCGGCAAGTCATGGTGACTCTGTCTCCTACAGA12620.11731164834020749No Hit
TCTATAAGGCGTCTAGTTTAGAGAGTGGGGTCCTATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT12040.11192014627702838No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA11970.11126944775216195No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11890.11052579229517173No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC11700.10875961058481995No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC11630.1081089120599535No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11630.1081089120599535No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA11590.1077370843314584No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11560.10745821353508707No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT11370.10569203182473527No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11270.1047624625034975No Hit
CCTTATAGATTAGGAGTTTGGGGACTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACCAATACTCTGA11160.10373993625013594No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11100.10318219465739328No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCTTGG11070.10290332386102194No Hit
CCATTGTCCCTTGGCCCCAGATATCAAAAGCATCAGTGTTAAGCCACTGCTTTCTATGATTCGCACAGTAATATA11050.1027174099967744No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGT10960.10188079760766039No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC10960.10188079760766039No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGGGA200.007719383651.1277718
CCACGTA400.00965242948.3137970
TCTGGAG15150.045.92201670
CGTATGC3050.044.1507641
CTTATAC2950.043.9206241
TCGTATG2800.043.10742640
TAAGACA3150.042.37205531
ATCTCGT2550.041.78455737
GTGGTAT2150.041.2327041
TATACAC3150.041.114813
ACGAGAC3250.040.92123822
CACGAGA3300.040.29372421
GTATCAA20650.039.7927471
TTATACA3350.039.6775672
GCGTATG350.001350475939.41250240
CCGTCTT3500.038.77549747
TCTCCGA3450.038.53107511
CCCTAAG3400.038.1661728
TCCGAGC3500.037.977113
CTAAGAC3550.037.5609330
Produced by FastQC (version 0.11.5)