FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1075767
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTATTCCGTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT56070.5212095184180218No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCGAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC53090.493508352645136No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41290.38381917273907823No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA39240.3647630016537038No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37140.34524204590771046No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30070.27952149489619965No Hit
GAGTATTGGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGTCCCCAAACTCCTAATCTATAAGGCGT28920.2688314477019652No Hit
AGTTTAGAGAGTGGGGTCCTATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC27500.2556315633403887No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25740.23927114328660387No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25710.23899227249023258No Hit
CTCTAAACTAGACGCCTTATAGATTAGGAGTTTGGGGACTTTCCCTGGTT25670.23862044476173744No Hit
GTATATTACTGTGCGAATCATAGAAAGCAGTGGCTTAACACTGATGCTTT24670.22932475154935966No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC24050.22356142175768545No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT21790.20255315509771168No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21750.20218132736921657No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21000.19520955745993324No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20820.19353633268170525No Hit
GTGTTAAGCCACTGCTTTCTATGATTCGCACAGTAATATACGGCCGTGTC20800.19335041881745768No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19580.1820096730983568No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19370.18005757752375748No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATGACTTGCCGGGCCAGTCAGA18670.173550592275093No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18420.1712266689719986No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18370.1707618843113797No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATGACTTGCCG18340.17048301351500836No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17730.16481264065545792No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17450.16220984655599213No Hit
GTAGGAGACAGAGTCACCATGACTTGCCGGGCCAGTCAGAGTATTGGTAGCTGGTTGGCCTGGTATCAGCAGAAA17390.16165210496324947No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16760.1557958182394515No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG16720.15542399051095637No Hit
CTCCTAATCTATAAGGCGTCTAGTTTAGAGAGTGGGGTCCTATCAAGGTT16660.1548662489182137No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16580.15412259346122348No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC16460.15300711027573813No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16180.15040431617627237No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16160.1502184023120248No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16080.1494747468550346No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTCGCAACTTA15930.14808039287317792No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15890.1477085651446828No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15800.14687195275556883No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT15790.14677899582344506No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14550.1352523362400966No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT14470.13450868078310638No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14300.13292841293700217No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATAGGACCCCA14250.13246362827638328No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14040.13051153270178395No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13810.12837352326293705No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC13760.12790873860231816No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13230.12298202119975794No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACTGGC12960.12047218403241594No Hit
ACTTATTACTGCCAACAGTATAATAGTTATTCCGTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA12870.11963557164330194No Hit
CAGTATAATAGTTATTCCGTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT12550.11666094981534106No Hit
CTACGTAGACTCCGTGAAGGGCCGCTTCACCATCTCCAGAGACAATCCCAAGAACACGCTGTATCTGCAAATGAA12410.11535955276560816No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA12390.1151736389013606No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12280.11415111264799906No Hit
GATTAGGAGTTTGGGGACTTTCCCTGGTTTCTGCTGATACCAGGCCAACC12250.11387224185162773No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATGACTTGCCGGGCCAGTCAGAGTATTGGTAG12140.11284971559826618No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12140.11284971559826618No Hit
GTAATAAGTTGCGAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG12030.11182718934490463No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12020.11173423241278083No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG12010.11164127548065705No Hit
GTATTGGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGTCCCC11940.1109905769557906No Hit
CCAATACTCTGACTGGCCCGGCAAGTCATGGTGACTCTGTCTCCTACAGA11660.10838778285632485No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11570.10755117046721083No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11530.10717934273871572No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11190.10401880704650729No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG11170.10383289318225972No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11150.10364697931801217No Hit
TCTATAAGGCGTCTAGTTTAGAGAGTGGGGTCCTATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT11130.10346106545376461No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC11100.10318219465739328No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11070.10290332386102194No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11040.10262445306465061No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCTTGG11020.10243853920040306No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA10860.10095122828642261No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)