FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences776171
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACCAGTCCGCTCACTTTCGGCGGAGGGACCAACGTGGAGATCAAACGAACTGTGGCTGCACCAT43380.55889745945159No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30180.38883184246770364No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27920.35971454743864434No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20540.26463240703401697No Hit
GTATAATATTGCAGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCT20310.2616691424956614No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20010.2578040148369367No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19780.2548407502985811No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19520.2514909729943531No Hit
ATTTTATACAACTCCGACAGTAAGAATTACTTAGCTTGGTTCCAGCAGAA18640.24015326519542732No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18050.2325518474666021No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17840.22984625810549478No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGGAACCAAGCTAAGTAATTCTTA16510.21271085881848203No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16410.21142248293224047No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15360.19789453612670405No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15140.1950601091769726No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14270.183851238966671No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14210.18307821343492606No Hit
CTCCTAAGTTGCTCATTAACTGGGCATCTACCCGGCAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTG13920.17934192336482555No Hit
GGTATAATATTGCAGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAAT13400.1726423687563694No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12880.1659428141479133No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12170.1567953453555982No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT11850.1526725425196252No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT11840.15254370493100103No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11800.1520283545765044No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11180.14404042408180673No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11120.14326739855006176No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11030.14210786025244437No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10970.14133483472069944No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC10940.14094832195482696No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10870.14004645883445788No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGCGACAGATTTCACTCTCAC10660.13734086947335059No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTAACTGGGCATCTACCCGGCAA10350.13334690422600173No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10330.13308922904875345No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCGGAGTATTTTATA10280.13244504110563265No Hit
GAATTACTTAGCTTGGTTCCAGCAGAAACCAGGACAGCCTCCTAAGTTGC10100.13012596451039785No Hit
GTATAAAATACTCCGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA10090.12999712692177368No Hit
GTTTATTATTGTCTGCAATATTATACCAGTCCGCTCACTTTCGGCGGAGGGACCAACGTGGAGATCAAACGAA9760.12574548649717654No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9550.12303989713606923No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9520.12265338437019678No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9320.12007663259771366No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9220.11878825671147207No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9220.11878825671147207No Hit
ATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGTCCACCT9100.11724220564798221No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9000.11595382976174065No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8990.1158249921731165No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCGGAGTATTTTATACAACTCCGA8780.1131194028120092No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8650.11144451415989517No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8580.11054265103952608No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8560.11028497586227778No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACGTTGGTCCCTCCGCCGAAAGTG8540.11002730068502946No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8440.1087389247987879No Hit
GTCCTGGTTTCTGCTGGAACCAAGCTAAGTAATTCTTACTGTCGGAGTTG8340.10745054891254634No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8320.10719287373529802No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8290.10680636096942556No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8220.10590449784905645No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8180.10538914749455984No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8180.10538914749455984No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8150.10500263472868737No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8120.10461612196281489No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT8110.10448728437419073No Hit
ATTCAGTGGCAGCGGGTCTGCGACAGATTTCACTCTCACCATCGGCAGCC8050.10371425884244581No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8030.10345658366519749No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8000.10307007089932502No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7990.10294123331070087No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7950.10242588295620425No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC7830.10087983189271436No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7810.10062215671546605No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCATAC200.00804277150.598371
GGTATCA7950.041.1575851
GTATCAA20100.039.102561
CGTATGC2600.035.85911641
TCGATAT750.002469249834.8493670
TCTGTCG7400.034.4372970
CTCTTAT1203.6379788E-1233.732251
TAGACAG502.1263074E-433.725735
ATCTCGT2050.033.384937
TGGTATC1950.032.860972
TATCAAC24050.032.8142242
ATCAACG24000.032.742063
TCGTATG2850.032.6425340
TCAACGC24100.032.60624
TATACAC2800.032.521243
GTGGCCG6250.032.40990470
AACGCAG24450.032.139456
CAACGCA24600.031.9434765
TTAACGG652.5190751E-531.46590635
ATGCCGT2900.031.13544344
Produced by FastQC (version 0.11.5)