FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985106
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC35510.36046882264446667No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG34590.35112972614114624No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA34100.34615564213394295No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT33080.33580142644547895No Hit
GTAATAGACAGCCGTGTCCTCGGCTCTCAGACTGTCCATTTGCAGATACA30450.3091037918762042No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG29390.2983435285136828No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC29240.2968208497359675No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28600.29032408695104894No Hit
GATTATTACTGCAGCTCATTTGGAGACAGCAGCAATTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT26560.2696156555741209No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATTGCTGCTGTCTCCAAATGAGCTGCAGTAATAAT26450.2684990244704631No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC24990.25367828436736756No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC24670.25042990297490825No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC23720.24078627071604475No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT22820.23165019804975304No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC22250.2258640186944349No Hit
GTCTATTACTGTGCAAGCAAGTTCGTCGCGGGCTTTGATATCTGGGGCCA21170.21490073149488484No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATTGCTGCTGTCTCCAAATGAGCTG20910.21226142161351164No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20710.2102311832432246No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATTGCTG20540.20850548062848062No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATTGCTGCTGT20070.203734420458306No Hit
GGATGAGGCTGATTATTACTGCAGCTCATTTGGAGACAGCAGCAATTATGTCTTCGGAACTGGGACCAAGGTCAC19920.2022117416805907No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGCTCTAACTTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGC19810.20109511057693283No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT19690.19987696755476062No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGCTCTAACTTTGTCTCCTGGTACCAACAGCA19330.1962225384882439No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19210.19500439546607165No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG18830.19114694256252626No Hit
GTAATAATCAGCCTCATCCTCCGCCTGGAGCCCAGAGACGGTCAGGGAGG17290.17551410711131593No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC16840.1709460707781701No Hit
TCTCTGGGCTCCAGGCGGAGGATGAGGCTGATTATTACTGCAGCTCATTT16580.1683067608967969No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG15500.1573434736972468No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG15420.15653137834913197No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG15380.15612533067507456No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT14830.15054217515678517No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA14550.14769984143838327No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG14430.14648169841621103No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT13920.14130459057197906No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC13620.13825923301654847No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT13420.13622899464626143No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA13410.13612748272774708No Hit
CCCCTGAGGGCCGCTTATTGACCTCATAAATCATGAGTCTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA13300.13501085162408918No Hit
GCCTCATCCTCCGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCC13240.13440178011300308No Hit
CATTTGGAGACAGCAGCAATTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACC12870.130645839127972No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC12750.12942769610579977No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC12710.12902164843174238No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT12670.12861560075768497No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGTGCCAGAGAAGCGATCAGGGAC12620.1281080411651132No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGG12540.12729594581699838No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT12470.1265853623873979No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCACCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGG12330.12516419552819696No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG12100.12282942140236686No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA12050.1223218618097951No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC12000.12181430221722334No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA11930.12110371878762285No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC11930.12110371878762285No Hit
GTCTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGACAAAGTTAGAG11590.11765231355813485No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG11520.1169417301285344No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG11290.11460695600270428No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11270.11440393216567558No Hit
GTCAATAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCACCAAGTCTGGCAACACGGCCTCCCTGACCGT11220.1138963725731038No Hit
GGCAAAGCCCCCAGACTCATGATTTATGAGGTCAATAAGCGGCCCTCAGG11210.11379486065458946No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11050.11217066995835982No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11020.11186613420281674No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCGGAGGATGAGGCTGATTATTACT11000.11166311036578805No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGTGCCAGAGAAG10970.11135857461024498No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC10670.1083132170548144No Hit
CTCCAAATGAGCTGCAGTAATAATCAGCCTCATCCTCCGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGC10660.10821170513630005No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT10640.10800868129927135No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCACCAAGTCTGGCAACACGG10450.10607995484749864No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC10380.10536937141789816No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC10330.1048618118253264No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10310.10465878798829771No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG10310.10465878798829771No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10020.10171494235138147No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG10020.10171494235138147No Hit
GTATCAACGCAGAGTACGGGGCTGGGGTCTCAGGAGGCAGCGCTCTCAGGACGTCACCACCATGGCCTGGGCT9980.10130889467732407No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)