FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985106
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC35520.36057033456298104No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG34730.3525508930003472No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT31430.31905195989061075No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA30980.31448392355746485No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29050.29489212328419484No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG28200.2862636102104748No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC27960.2838273241661303No Hit
GTAATAGACAGCCGTGTCCTCGGCTCTCAGACTGTCCATTTGCAGATACA27630.2804774308551567No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC25920.26311889278920236No Hit
GATTATTACTGCAGCTCATTTGGAGACAGCAGCAATTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT25470.2585508564560565No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24340.24708000966393462No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATTGCTGCTGTCTCCAAATGAGCTGCAGTAATAAT24230.24596337856027675No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC22600.22941693584243728No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT22230.22566099485740618No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC21990.22322470881306175No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC21440.21764155329477233No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATTGCTG20520.2083024567914519No Hit
GTCTATTACTGTGCAAGCAAGTTCGTCGCGGGCTTTGATATCTGGGGCCA20390.20698280185076529No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20090.20393744429533472No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGCTCTAACTTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGC20080.20383593237682038No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATTGCTGCTGTCTCCAAATGAGCTG19800.2009935986584185No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG18890.19175601407361237No Hit
GGATGAGGCTGATTATTACTGCAGCTCATTTGGAGACAGCAGCAATTATGTCTTCGGAACTGGGACCAAGGTCAC18790.19074089488846885No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATTGCTGCTGT18730.19013182337738274No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT18080.1835335486739498No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18050.18322901291840674No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGCTCTAACTTTGTCTCCTGGTACCAACAGCA17360.1762246905409164No Hit
TCTCTGGGCTCCAGGCGGAGGATGAGGCTGATTATTACTGCAGCTCATTT16720.16972792775599785No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG15910.16150546235633526No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC15820.16059185508970608No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT15450.15683591410467504No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG15220.1545011399788449No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT15060.15287694928261528No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG14760.1498315917271847No Hit
GTAATAATCAGCCTCATCCTCCGCCTGGAGCCCAGAGACGGTCAGGGAGG14620.14841042486798375No Hit
CCCCTGAGGGCCGCTTATTGACCTCATAAATCATGAGTCTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA13900.14110156673495033No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA13680.1388683045276346No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC13620.13825923301654847No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG13580.13785318534249108No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC13480.13683806615734753No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT13400.1360259708092327No Hit
CATTTGGAGACAGCAGCAATTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACC12490.1267883862244266No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC12260.1244536120985965No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT12090.1227279094838525No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC12000.12181430221722334No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA11840.12019011152099368No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA11830.12008859960247933No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC11680.11856592082476404No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGG11300.11470846792121864No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCGGAGGATGAGGCTGATTATTACT11170.11338881298053204No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC11100.11267822955093158No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGTGCCAGAGAAGCGATCAGGGAC11090.11257671763241722No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT10960.11125706269173062No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10840.11003891966955841No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCACCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGG10840.11003891966955841No Hit
GTCTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGACAAAGTTAGAG10740.10902380048441489No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG10730.10892228856590053No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC10690.10851624089184313No Hit
GTCAATAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCACCAAGTCTGGCAACACGGCCTCCCTGACCGT10500.1065875144400704No Hit
GCCTCATCCTCCGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCC10210.10364366880315419No Hit
GGCAAAGCCCCCAGACTCATGATTTATGAGGTCAATAAGCGGCCCTCAGG10180.10333913304761111No Hit
GTATCAACGCAGAGTACGGGGCTGGGGTCTCAGGAGGCAGCGCTCTCAGGACGTCACCACCATGGCCTGGGCT10170.10323762112909676No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG10050.10201947810692454No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA10050.10201947810692454No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC10050.10201947810692454No Hit
CTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC9880.10029377549218053No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT9860.10009075165515183No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)