FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1749554
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCTCCCCGAACACTTTTGGCCAGGGGA81840.46777635900349457No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCCCCGAACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA66200.37838214767877987No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64950.3712374696636972No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC61930.3539759275792574No Hit
CAGTAATACACTGCGAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA53860.30784988631388344No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA44780.25595094521232264No Hit
CTCCTGCAGGGCCGGTCAGAGTGTTCGCAGCAGCTACTTAGCCTGGTACC41610.23783204176607295No Hit
GTAATACACTGCGAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA41250.23577437449772914No Hit
GTATGGTAGCTCCCCGAACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT40450.23120178056807622No Hit
CATCTATGGTGTATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA36510.20868175546453552No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35990.20570956941026114No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35570.20330895759719333No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33720.19273483413487094No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCGGTCAGAGTGTTCGCAG33550.19176315792481968No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33110.18924823126351056No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA31440.17970294143536011No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30990.17713085734993034No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30920.17673075538108568No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT30770.1758733940192758No Hit
GTAATACAAGGCAGTGTCCTCAGTTCTCAGACTGTTCATTTGCACATACA29920.17101501296901953No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29560.16895734570067572No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29160.16667104873584926No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCGGTCAGA28250.16146972314086905No Hit
CTACCATACTGCTGACAGTAATACACTGCGAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC28130.1607838340514211No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC28090.16055520435493847No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT27920.15958352814488722No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCCGGTACCAGGCTAAGTAGCTGCTGCGAACACTCTGA27830.15906911132780127No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27240.15569682330468224No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27150.15518240648759626No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA27000.15432504512578635No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26660.15238169270568386No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC25940.14826635816899622No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT25770.14729468195894496No Hit
GTATTACTGTGCAAAAGATAGCGCGGATTACGTTTGGGGGAGTTATCTTGACTACTGGGGCCAGGGAACCCTGGT25360.14495122756999784No Hit
GAGTTTGGACTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGT24020.13729213273782917No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT23420.13386268729058948No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23330.13334827047350353No Hit
GGCCAAAAGTGTTCGGGGAGCTACCATACTGCTGACAGTAATACACTGCG23030.13163354774988367No Hit
CTCCTGTGCAGCCTCTGGATTCAGTTTTGATGATTATACCATGCACTGGGTCCGTCAAGCTCCGGGGAAGGGT22960.13123344578103907No Hit
CTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACAGCAAAAACTCCCTGTATGTGCAAATGAA22850.13060471411571178No Hit
GGTCTGGAGTGGGTCTCCCTTATTAGTTGGGATGGTGGTAGCACACGCTA22810.13037608441922913No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG22640.12940440820917787No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC22310.12751821321319604No Hit
CAGTAATACAAGGCAGTGTCCTCAGTTCTCAGACTGTTCATTTGCACATA22280.12734674094083406No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22250.12717526866847206No Hit
TGATTATACCATGCACTGGGTCCGTCAAGCTCCGGGGAAGGGTCTGGAGT22090.1262607498825415No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG22040.1259749627619382No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT22030.12591780533781752No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG21690.12397445291771503No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21550.12317424898002577No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC21100.12060216489459599No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC21030.12020206292575135No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT21010.12008774807751005No Hit
TCTATGGTGTATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT20820.11900175701921747No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20360.11637251550966703No Hit
GCACTGAACACAGAGAACGCACCATGGAGTTTGGACTGAGCTGGGTTTTC20320.11614388581318438No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20260.11580094126846041No Hit
GTCTGAGAACTGAGGACACTGCCTTGTATTACTGTGCAAAAGATAGCGCG20200.11545799672373644No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20130.11505789475489182No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20080.11477210763428851No Hit
GTTCTCAGACTGTTCATTTGCACATACAGGGAGTTTTTGCTGTTGTCTCT19880.11362895915187528No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA19810.11322885718303063No Hit
ATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACAGCAAA19760.11294307006242733No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19670.11242865324534138No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTCGCAGTGTATTACTGTCAGCAGTATGGTAGCTCC19630.11220002354885873No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19610.11208570870061743No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGAAGAAATTGTGTTGACGCAGTCTC19130.10934215234282565No Hit
GCCTTGTATTACTGTGCAAAAGATAGCGCGGATTACGTTTGGGGGAGTTATCTTGACTACTGGGGCCAGGGAACC18770.10728448507448185No Hit
CTCCAGACCCTTCCCCGGAGCTTGACGGACCCAGTGCATGGTATAATCAT18720.10699869795387854No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC18680.10677006825739589No Hit
CCCTTATTAGTTGGGATGGTGGTAGCACACGCTATGCAGACTCTGTGAAG18660.10665575340915456No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18470.10556976235086199No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18390.1051125029578967No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17980.10276904856894957No Hit
CTGCTACTCTGGCTCCCAGATACCACCGAAGAAATTGTGTTGACGCAGTC17870.10214031690362228No Hit
CTGCAGGGCCGGTCAGAGTGTTCGCAGCAGCTACTTAGCCTGGTACCGGCAGAAACCTGGCCAGGCTCCCAGGCT17790.10168305751065701No Hit
GTCAGAGTGTTCGCAGCAGCTACTTAGCCTGGTACCGGCAGAAACCTGGC17630.10076853872472641No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA17540.10025412190764046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG3750.049.6698840
CGCTAAT3500.048.8811132
TCGCTAA3850.047.9634631
ACACTCG3900.047.2536727
CGTATGC4100.046.33808541
GCTAATC3500.045.97067633
ACTCGCT4050.045.54786329
ATCTCGT3550.045.48645837
CGAGACA4050.045.44629323
TCTCCGA4300.044.37798311
AATCTCG3650.044.1867836
GTATGCC4500.043.0391442
CACTCGC4300.042.87761728
GGTATCA13850.042.5800131
ATGCCGT4500.042.3787644
ATCTCCG4550.041.93963610
CTTATAC4400.041.8360331
ACGAGAC4400.041.82646622
CTAATCT4000.040.25901834
TGTACGA459.8735836E-538.42780342
Produced by FastQC (version 0.11.5)