FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1749554
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCTCCCCGAACACTTTTGGCCAGGGGA78480.4485714644989523No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCCCCGAACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA64590.3691798023953533No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64570.369065487547112No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55100.3149374069048455No Hit
CAGTAATACACTGCGAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA52590.3005908934505594No Hit
GTAATACACTGCGAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA41590.2377177269178316No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40920.23388817950174728No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA39300.22462867679420012No Hit
CTCCTGCAGGGCCGGTCAGAGTGTTCGCAGCAGCTACTTAGCCTGGTACC39090.22342837088766623No Hit
GTATGGTAGCTCCCCGAACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT38580.22051334225751248No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35070.20045108639116024No Hit
CATCTATGGTGTATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA34500.19719311321628255No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33340.19056285201828582No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31990.18284659976199646No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31170.17815969098410223No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCGGTCAGAGTGTTCGCAG30750.17575907917103445No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT30600.17490171780922453No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30260.17295836538912202No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29950.1711864852413815No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA28720.16415612207454014No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT28640.16369886268157485No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC28200.16118393602026573No Hit
CTACCATACTGCTGACAGTAATACACTGCGAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC27790.15884048163131861No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCCGGTACCAGGCTAAGTAGCTGCTGCGAACACTCTGA27610.1578116479971467No Hit
GTAATACAAGGCAGTGTCCTCAGTTCTCAGACTGTTCATTTGCACATACA27250.1557539807288029No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCGGTCAGA27200.15546819360819958No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26530.15163864619211526No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC26080.14906656210668548No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25370.1450083849941185No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24890.14226482863632675No Hit
GTATTACTGTGCAAAAGATAGCGCGGATTACGTTTGGGGGAGTTATCTTGACTACTGGGGCCAGGGAACCCTGGT24710.14123599500215483No Hit
GAGTTTGGACTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGT24230.1384924386443631No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24210.13837812379612174No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24200.1383209663720011No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG23920.13672055849662257No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23830.13620614167953662No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23360.13351974274586553No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT23300.13317679820114156No Hit
CTCCTGTGCAGCCTCTGGATTCAGTTTTGATGATTATACCATGCACTGGGTCCGTCAAGCTCCGGGGAAGGGT23260.1329481685046589No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23240.1328338536564176No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC22680.12963303790566053No Hit
GGCCAAAAGTGTTCGGGGAGCTACCATACTGCTGACAGTAATACACTGCG22410.12808978745440266No Hit
CTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACAGCAAAAACTCCCTGTATGTGCAAATGAA21890.12511760140012826No Hit
GTCTGAGAACTGAGGACACTGCCTTGTATTACTGTGCAAAAGATAGCGCG21840.12483181427952496No Hit
TGATTATACCATGCACTGGGTCCGTCAAGCTCCGGGGAAGGGTCTGGAGT21520.12300277670766377No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21500.12288846185942247No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21060.12037353519811335No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACTGGC20510.11722987687147696No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20440.11682977490263234No Hit
TCTATGGTGTATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT20080.11477210763428851No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT19930.11391474627247859No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA19920.11385758884835792No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA19870.1135718017277546No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC19850.1134574868795133No Hit
GCACTGAACACAGAGAACGCACCATGGAGTTTGGACTGAGCTGGGTTTTC19550.11174276415589345No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG19500.11145697703529014No Hit
CTGCTACTCTGGCTCCCAGATACCACCGAAGAAATTGTGTTGACGCAGTC19500.11145697703529014No Hit
CAGTAATACAAGGCAGTGTCCTCAGTTCTCAGACTGTTCATTTGCACATA19490.11139981961116947No Hit
GGTCTGGAGTGGGTCTCCCTTATTAGTTGGGATGGTGGTAGCACACGCTA19290.11025667112875624No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19240.10997088400815293No Hit
ATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACAGCAAA19120.109284994918705No Hit
GTTCTCAGACTGTTCATTTGCACATACAGGGAGTTTTTGCTGTTGTCTCT19080.10905636522222234No Hit
CCCTTATTAGTTGGGATGGTGGTAGCACACGCTATGCAGACTCTGTGAAG18990.1085419484051364No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTCGCAGTGTATTACTGTCAGCAGTATGGTAGCTCC18700.1068843831056372No Hit
CTCCAGACCCTTCCCCGGAGCTTGACGGACCCAGTGCATGGTATAATCAT18400.10516966038201735No Hit
GCCTTGTATTACTGTGCAAAAGATAGCGCGGATTACGTTTGGGGGAGTTATCTTGACTACTGGGGCCAGGGAACC18260.10436945644432809No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC18230.10419798417196612No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18210.10408366932372479No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17830.10191168720713964No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17820.10185452978301898No Hit
CTGCAGGGCCGGTCAGAGTGTTCGCAGCAGCTACTTAGCCTGGTACCGGCAGAAACCTGGCCAGGCTCCCAGGCT17650.10088285357296775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13800.040.9189761
CTAAACG450.0015582638.28212769
GTATCAA34700.037.1539961
GGTACTA751.2709224E-633.34527260
TATCAAC39550.032.564272
ATCAACG39650.032.3079643
TCAACGC39750.032.226694
AACGCAG40400.031.7968376
AGTCGCG652.4146237E-531.66482735
CAACGCA40450.031.5858945
TACGTAC1950.031.36977412
AACTGTG30200.030.803970
ATACGTA2100.030.74735611
ACGCAGA42000.030.6664437
CGCAGAG42300.030.288278
AGAGTAC42850.029.74088911
GCAGAGT43850.029.0626499
CGTACCA2150.028.45734814
GAATACG2150.028.4516569
GCGTAGA30500.028.1044170
Produced by FastQC (version 0.11.5)