FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666380
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25700.38566583630961315No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24480.3673579639244875No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19320.28992466760707103No Hit
GAGTTCACTCTCACCATCGACGATCTGCAACCTGAAGACATCGCAACTTATTACTGTCAACAGAGTTACGGTAAC17230.2585611813079624No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16180.24280440589453464No Hit
GAGTTACGGTAACCCTCGGACTTTTGGCCAGGGGACCAAACTGGACATCAAACGAACTGTGGCTGCACCAT16180.24280440589453464No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15650.2348509859239473No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15100.2265974368978661No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14450.21684324259431553No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13990.20994027431795673No Hit
GTCTGAGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCCAGACCTCTGGATACACTTTCAACGA13770.20663885470752424No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13680.20528827395780183No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13250.19883549926468383No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13120.19688466040397373No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13010.19523395059875748No Hit
CTGCTACTCTGGCTCCGAGATGCCAGATGTGACATCCAGCTGGACCAGTC12580.18878117590563942No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12260.18397911101773762No Hit
ACTTATTACTGTCAACAGAGTTACGGTAACCCTCGGACTTTTGGCCAGGGGACCAAACTGGACATCAAACGAA12190.1829286593235091No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12130.18202827215702752No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11950.17932711065758275No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT11430.17152375521474233No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11340.17017317446501995No Hit
GTGCAGCCACAGTTCGTTTGATGTCCAGTTTGGTCCCCTGGCCAAAAGTC11100.16657162579909363No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10750.16131936732795102No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10720.16086917374471021No Hit
ATATGGGACAGAGTTCACTCTCACCATCGACGATCTGCAACCTGAAGACA10680.1602689156337225No Hit
GAGTGAACTCTGTCCCATATCCACTGCCACTGAACTTTGACGGGACCCCC10580.15876827035625318No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10400.15606710885680844No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGATGCCAGATGTGACAT10170.15261562471862902No Hit
GTCCCATATCCACTGCCACTGAACTTTGACGGGACCCCCTCTTGCAAGAT10170.15261562471862902No Hit
GGATATGGGACAGAGTTCACTCTCACCATCGACGATCTGCAACCTGAAGA10150.15231549566313515No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCAGTTTGGTCC10080.15126504396890664No Hit
CCTGAAGACATCGCAACTTATTACTGTCAACAGAGTTACGGTAACCCTCG9850.14781355983072722No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9650.14481226927578858No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9590.143911882109307No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9510.14271136588733155No Hit
GTAATAAGTTGCGATGTCTTCAGGTTGCAGATCGTCGATGGTGAGAGTGAACTCTGTCCCATATCCACTGCCA9380.14076052702662145No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC9130.13700891383294816No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9110.1367087847774543No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9060.13595846213871965No Hit
GTCTTCAGGTTGCAGATCGTCGATGGTGAGAGTGAACTCTGTCCCATATCCACTGCCACTGAACTTTGACGGGAC8870.13310723611152797No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8730.13100633272307094No Hit
CCGTAACTCTGTTGACAGTAATAAGTTGCGATGTCTTCAGGTTGCAGATCGTCGATGGTGAGAGTGAACTCTGTC8690.1304060746120832No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGATGCCAGATGTGACATCC8580.12875536480686695No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8480.12725471952939765No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8340.1251538161409406No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8170.12260271916924277No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8050.1208019448362796No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7980.11975149314205108No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC7840.11765058975359405No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7820.11735046069810018No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT7750.11630000900387166No Hit
CCATCGTCCTTGTCCGCATCGGTGGGAGATAGAATCACCATCACTTGCCG7680.11524955730964316No Hit
GTCCTTGTCCGCATCGGTGGGAGATAGAATCACCATCACTTGCCGGGCAA7670.11509949278189621No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7640.11464929919865541No Hit
GTCTCCATCGTCCTTGTCCGCATCGGTGGGAGATAGAATCACCATCACTTGCCGGGCAAGCCTCGTCATTAGCGC7610.11419910561541462No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7590.11389897655992078No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7570.1135988475044269No Hit
GTTTGGTCCCCTGGCCAAAAGTCCGAGGGTTACCGTAACTCTGTTGACAG7540.1131486539211861No Hit
CCCCAGGACAAAGGCCTGAGTGGATGGGACGGATCCTCCCTAAGAACGGT7390.11089768600498215No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC7350.11029742789399441No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7290.10939704072751283No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCG7270.10909691167201897No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT7140.10714607281130886No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7070.10609562111708036No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7040.10564542753383956No Hit
ACAGAAGCCTGGGAAAGCCCCTCAACTCCTGATCTATCATGCCTCCATCTTGCAAGAGGGGGTCCCGTCAAAGTT6980.10474504036735797No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC6980.10474504036735797No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6970.10459497583961103No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6950.10429484678411717No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6940.10414478225637024No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6910.10369458867312943No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGATGCCAGATGTGACATCCAG6860.10294426603439479No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6780.10174374981241933No Hit
ATCATGCCTCCATCTTGCAAGAGGGGGTCCCGTCAAAGTTCAGTGGCAGTGGATATGGGACAGAGTTCACTCTCA6760.10144362075692548No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC6690.10039316906269696No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)