FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666380
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25490.38251448122692755No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24090.3615054473423572No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21410.32128815390617965No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18740.28122092499774903No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16180.24280440589453464No Hit
GAGTTACGGTAACCCTCGGACTTTTGGCCAGGGGACCAAACTGGACATCAAACGAACTGTGGCTGCACCAT15240.22869834028632313No Hit
GAGTTCACTCTCACCATCGACGATCTGCAACCTGAAGACATCGCAACTTATTACTGTCAACAGAGTTACGGTAAC15030.22554698520363756No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14710.22074492031573575No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14310.2147423392058585No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14200.21309162940064225No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14050.2108406614844383No Hit
GTCTGAGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCCAGACCTCTGGATACACTTTCAACGA12880.19328311173804738No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12380.1857798853507008No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12080.18127794951829285No Hit
ACTTATTACTGTCAACAGAGTTACGGTAACCCTCGGACTTTTGGCCAGGGGACCAAACTGGACATCAAACGAA11700.1755754974639095No Hit
CTGCTACTCTGGCTCCGAGATGCCAGATGTGACATCCAGCTGGACCAGTC11640.1746751102974279No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11530.17302440049221166No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG11530.17302440049221166No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11160.1674720129655752No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11150.16732194843782827No Hit
GAGTGAACTCTGTCCCATATCCACTGCCACTGAACTTTGACGGGACCCCC10810.1622197544944326No Hit
ATATGGGACAGAGTTCACTCTCACCATCGACGATCTGCAACCTGAAGACA10570.15861820582850625No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10510.15771781866202467No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10490.15741768960653083No Hit
GTGCAGCCACAGTTCGTTTGATGTCCAGTTTGGTCCCCTGGCCAAAAGTC10430.15651730244004922No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10400.15606710885680844No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10340.15516672169032683No Hit
GTAATAAGTTGCGATGTCTTCAGGTTGCAGATCGTCGATGGTGAGAGTGAACTCTGTCCCATATCCACTGCCA10060.15096491491341277No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9860.14796362435847416No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9710.14571265644227016No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCAGTTTGGTCC9640.14466220474804165No Hit
GTCCCATATCCACTGCCACTGAACTTTGACGGGACCCCCTCTTGCAAGAT9510.14271136588733155No Hit
GGATATGGGACAGAGTTCACTCTCACCATCGACGATCTGCAACCTGAAGA9350.14031033344338065No Hit
GAAGTAGACGGCCGTGTCGTCAGATGTGAGGCCGCTCACCTCCAAGTAGACAGTGGCGATGGACGTGTCGCTGGT9340.14016026891563374No Hit
GTCTTCAGGTTGCAGATCGTCGATGGTGAGAGTGAACTCTGTCCCATATCCACTGCCACTGAACTTTGACGGGAC9310.13971007533239294No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9210.13820943005492362No Hit
CCTGAAGACATCGCAACTTATTACTGTCAACAGAGTTACGGTAACCCTCG9190.13790930099942975No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8900.13355742969476875No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGATGCCAGATGTGACAT8810.13220684894504636No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8700.13055613913983014No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC8630.1295056874456016No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8410.1262042678351691No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8160.12245265464149585No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGATGCCAGATGTGACATCC8160.12245265464149585No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7980.11975149314205108No Hit
CCGTAACTCTGTTGACAGTAATAAGTTGCGATGTCTTCAGGTTGCAGATCGTCGATGGTGAGAGTGAACTCTGTC7940.11915123503106337No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT7890.1184009123923287No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7870.11810078333683485No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7860.1179507188090879No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7730.1159998799483778No Hit
CCATCGTCCTTGTCCGCATCGGTGGGAGATAGAATCACCATCACTTGCCG7630.11449923467090849No Hit
GTCCTTGTCCGCATCGGTGGGAGATAGAATCACCATCACTTGCCGGGCAA7600.1140490410876677No Hit
GTTTGGTCCCCTGGCCAAAAGTCCGAGGGTTACCGTAACTCTGTTGACAG7580.11374891203217383No Hit
GTCTCCATCGTCCTTGTCCGCATCGGTGGGAGATAGAATCACCATCACTTGCCGGGCAAGCCTCGTCATTAGCGC7510.11269846033794531No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7470.11209820222695759No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA7360.11044749242174136No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7360.11044749242174136No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7230.10849665356103125No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7180.10774633092229659No Hit
ATCATGCCTCCATCTTGCAAGAGGGGGTCCCGTCAAAGTTCAGTGGCAGTGGATATGGGACAGAGTTCACTCTCA7150.1072961373390558No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7090.1063957501725742No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC7070.10609562111708036No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7040.10564542753383956No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6970.10459497583961103No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCG6820.10234400792340707No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6810.10219394339566013No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC6790.10189381434016628No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCAAA200.00792353750.7891225
CGTTTGT200.00798969750.68240413
GTACGGA200.00798969750.68240414
TTCCGCA306.963614E-445.0882519
GACCGGA350.001179666340.50899553
CTTTACT350.001450858138.8395930
CGCATTT350.001483986938.6615922
CTATTAA451.1258442E-437.5763631
AATGGTA1454.098365E-836.7051970
GAGTAAT501.4752071E-435.88892758
CCGCATT400.00286112533.82126621
ATCAACG15300.032.4608043
TCAACGC15350.032.1349644
GTGGTAT1600.031.7050531
CAACGCA15750.031.3188445
AACGCAG16000.031.2518066
GTCGTAA450.004281494331.14898748
TACTATT553.4825475E-431.00511733
CATATGT450.004453774530.89889144
GCTCGGC450.00497430230.20856930
Produced by FastQC (version 0.11.5)