FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1111096
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49150.4423560160418181No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48310.43479591322441985No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34770.31293425590588037No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33450.301054094335683No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30240.2721637014263394No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30230.27207370020232274No Hit
ATATTATCTTACTCCGACATTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT29550.2659536169691908No Hit
GTTTGGGAGGCTGTCCTGGCTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGGGGAATAAAAGACT29160.2624435692325416No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28590.2573134994635927No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28250.25425345784702674No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27780.2500234003182443No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27330.2459733452374952No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATT26510.23859324486813022No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26420.2377832338519804No Hit
CTCCCAAACTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT26120.23508319713148096No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26060.23454318978738112No Hit
CTTTTATTCCCCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA24840.2235630404573502No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24100.21690294988011838No Hit
AGCCAGGACAGCCTCCCAAACTGCTCATTTACTGGGCATCTACCCGGGAA23130.20817283115050364No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC22960.20664281034222065No Hit
GTCCTGGCTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGGGG22770.20493278708590437No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21960.197642687940556No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21280.19152260470742402No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19830.17847242722501025No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19730.1775724149848438No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19500.17550238683246092No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCACCAATATTATCTTACT19190.17271234888794487No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT19100.17190233787179504No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18880.16992231094342883No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATTCCCCTCCAA18870.16983230971941218No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAATGTC18600.16740227667096272No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC18020.1621822056779972No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17610.15849215549331472No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC17590.1583121530452814No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17230.15507210898068213No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC16920.1522820710361661No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16320.1468819975951673No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16020.1441819608746679No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGTTTGGGAGGCTGTCCTGGCTTCTGCTGGTA15160.13644185560923627No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15080.13572184581710312No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT15070.13563184459308647No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14960.13464183112890335No Hit
GTTTATTTCTGTCACCAATATTATCTTACTCCGACATTCGGCCAAGGGAC14890.13401182256078684No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14860.13374181888873687No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT14830.13347181521668694No Hit
ATCTTACTCCGACATTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACT14640.13176179196037066No Hit
GTAAATGAGCAGTTTGGGAGGCTGTCCTGGCTTCTGCTGGTACCAAGCTA14510.13059177604815425No Hit
GAATAAAAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA14360.12924175768790455No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14360.12924175768790455No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14170.12753173443158827No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13980.12582171117527197No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13930.12537170505518874No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC13870.12483169771108887No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGGCCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG13700.12330167690280588No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13600.12240166466263942No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATTCCCCTCCAACAATAAGAACTA13540.12186165731853953No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13440.12096164507837306No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13440.12096164507837306No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC13430.12087164385435642No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13310.11979162916615667No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13290.11961162671812336No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13170.11853161202992361No Hit
AGATAATATTGGTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC13070.11763159978975715No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCAGTTTCAATAATGCCTGG13070.11763159978975715No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13020.11718159366967391No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12810.11529156796532432No Hit
CTACTTAGCTTGGTACCAGCAGAAGCCAGGACAGCCTCCCAAACTGCTCATTTACTGGGCATCTACCCGGGAATC12810.11529156796532432No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12790.11511156551729104No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12740.1146615593972078No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12540.11286153491687487No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12530.11277153369285824No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12360.11124151288457523No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCACCAATATTATCTTACTCCGACATTCGG12330.1109715092125253No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12300.11070150554047535No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGATTTTC12290.1106115043164587No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC12230.11007149697235881No Hit
CTACGGGGACATCGTGATGGCCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG11980.10782146637194265No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11950.10755146269989273No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11940.10746146147587608No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11870.10683145290775954No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11830.10647144801169295No Hit
GGCTGAAGATGTGGCAGTTTATTTCTGTCACCAATATTATCTTACTCCGA11780.10602144189160972No Hit
GTACCAGCAGAAGCCAGGACAGCCTCCCAAACTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGA11750.10575143821955979No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11620.10458142230734338No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11470.10323140394709368No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG11260.10134137824274411No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11220.10098137334667752No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC11150.10035136477856098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG3700.045.6768640
GTACTAG3700.044.1176430
ACTAGAT3500.043.8188132
ATCTCGT3550.043.47740637
GTATGCC3900.043.47198542
ACGAGAC3900.043.44009822
CGTATGC3900.043.4140841
CGTACTA3700.043.17510229
ACCGTAC3700.043.12249427
TATACAC4100.042.9410323
TACTAGA3750.042.666931
GACCGTA3750.042.5283426
CTAGATC3550.042.28375233
ATGCCGT4100.041.49453744
ATCTCCG4000.041.47152710
TGGTATC2450.041.457982
TCTCCGA4050.040.95952611
GGTATCA7900.040.7273671
ATACACA4250.040.6288224
GAGACCG3950.040.33134524
Produced by FastQC (version 0.11.5)