FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1530243
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68930.4504513335463714No Hit
GTAATATACAGACGTGTCCTCGGCCCTCAGACTGTTCATATGCAGATACA58810.38431804621880317No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48440.31655103143749064No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46590.304461448279783No Hit
ATGTTATAGTAGTCCGCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT39970.26120034530463465No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36460.23826281185406498No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG34970.22852579622974914No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34060.22257902829811999No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32700.21369155094975112No Hit
GTATATTACTGTGTAAGAGGGGATGGTCCCCTCTGGCACCTCAGTCTCTGGGGCCGTGGCACCCTGGTCG32170.21022804874781328No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31370.20500012089583156No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28680.18742121349354318No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC28510.18631027882499707No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG27100.17709605598587938No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26860.17552767763028487No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA26700.17448209205988854No Hit
CTTTTATACGGCCCCAACAATAAGAATTATTTTGCTTGGTTCCAGCAGAA26580.17369790288209128No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26220.17134533534869953No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25170.16448368004297356No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24380.15932110128914168No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24240.15840621391504486No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24130.15768737383539738No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24100.15749132654094808No Hit
GTTTATTACTGTCAGCAATGTTATAGTAGTCCGCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAA22870.14945338746852624No Hit
GTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGCCAGAGTCTTT22200.1450749978924916No Hit
GTTATAGTAGTCCGCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAA21830.14265708126095006No Hit
CTATAACATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC21370.13965102274606062No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGGAACCAAGCAAAATAATTCTTATTGTTGGGGCCGTATAAAAGACT21260.1389321826664131No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC20600.13461914218852822No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT20130.131547734575489No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20130.131547734575489No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20010.13076354539769175No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAATTACTGGATGCACTGGGTCCGCCAAGTTCCAGGGAAGGGG19810.1294565634346963No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19810.1294565634346963No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19600.12808423237355113No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC19450.12710399590130456No Hit
ACTATAACATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT19220.12560096664385983No Hit
CTCCTAAGTTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCT18970.12396723919011557No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA18950.12383654099381602No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18450.12056908608632746No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18410.12030768969372838No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG18340.11985024600668No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG17720.11579860192139418No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17520.11449161995839878No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC17000.11109346685461068No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATGTTATAGTAGT16890.1103746267749632No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16620.10861020112491937No Hit
ATAGTAGTCCGCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGA16590.10841415383047005No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16350.10684577547487556No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC15660.10233668770254137No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG15520.10142180032844457No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15460.10102970573954594No Hit
GGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTG15380.10050691295434777No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAA251.927008E-458.50000462
CTTAACG252.0579038E-457.7273858
TACGTTA252.3874457E-456.0180947
TTTCGCG200.00807513450.54934720
AACGCTA304.8269E-448.57238861
TTAACGC304.9846893E-448.25660759
GGTATCA15550.046.5851141
GTGGTAT3950.041.796321
TGGTATC3950.041.7580682
ATTACGT350.001283722239.8220445
AATTACG350.001296155639.7440544
GTATCAA39100.039.2079121
AATGGTA2700.037.04276370
TTAACGG1350.035.4158835
TATCAAC46700.032.6529772
ATCAACG47300.032.0873
TCAACGC47350.031.9820444
CAACGCA48000.031.6902075
AACGCAG48450.031.6114836
GGGATAT850.0041316331.37739870
Produced by FastQC (version 0.11.5)