FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008542
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACG38990.38659768259527116No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC38430.3810451126477628No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG32850.32571771924223286No Hit
GCCTTGGGCTGACCTAGGACGCTCAGCTTGGTCCCTCCGCCGAATACCAC31600.3133235898951159No Hit
GACCTAGGACGCTCAGCTTGGTCCCTCCGCCGAATACCACTGAATGATCA30950.3068786426346151No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29330.2908158510007516No Hit
GGATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC28450.2820903839403813No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27400.271679315288803No Hit
CCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCT26140.25918603290690917No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGCTCAGCTTGGTCCCTC25900.2568063600722627No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23420.23221640744758276No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22950.2275562148130668No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG21650.21466632029206517No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21380.21198918835308792No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTACTT20960.20782476089245663No Hit
CTACTACCATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGCCTTC20310.20137981363195584No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG19750.19582724368444748No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT19580.19414164209323956No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG19560.1939433360236857No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19480.1931501117454702No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19020.18858907214573117No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCAGGATTAC18550.1839288795112152No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18330.18174751274612264No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC17670.1752034124508449No Hit
GTAGTAGTGATCATTCAGTGGTATTCGGCGGAGGGACCAAGCTGAGCGTCCTAGGTCAGCCCAAGGCTGCCCCCT17390.17242712747709069No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17320.17173305623365215No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17160.17014660767722117No Hit
ATAATACACAGCCCTGTCCTCGGCTCTCAGGCTGTTCATCTGCAGATGCA17030.16885761822512102No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17010.16865931215556715No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG16690.16548641504270523No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG16460.16320589524283569No Hit
GTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCG16310.1617185997211817No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC16300.16161944668640474No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16080.15943807992131215No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA15930.15795078439965812No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGA15420.1528939796260344No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGGCGGGGATGAGGCCGACTAT15370.15239821445214974No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15340.15210075534781892No Hit
GTGTATTATTGTGCGAGAGATCGGGCGGATACGTATTACAATGATGGTAG15330.152001602313042No Hit
TGGTAGTAGTGATCATTCAGTGGTATTCGGCGGAGGGACCAAGCTGAGCGTCCTAGGTCAGCCCAAGGCTGCCCC15180.15051430679138797No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14860.14734140967852602No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14810.14684564450464135No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA14640.14516004291343346No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA14640.14516004291343346No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT14220.14099561545280218No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCAG14190.14069815634847135No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14070.13950831993114812No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13970.1385167895833788No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGG13720.13603796371395538No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13330.13217099535765492No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13190.13078285287077782No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT13100.1298904755577854No Hit
CTATTACTGTCAGGTGTGGGATGGTAGTAGTGATCATTCAGTGGTATTCG13030.12919640431434684No Hit
CACTACTACCATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGCCTTCGACCCTGCTGATGGTCAGGGTGG12840.12731249665358507No Hit
GTAATAGTCGGCCTCATCCCCGCCTTCGACCCTGCTGATGGTCAGGGTGG12660.12552774202760023No Hit
GTGGTATTCGGCGGAGGGACCAAGCTGAGCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG12490.12384214043639234No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGCTCAGC12490.12384214043639234No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG12460.12354468133206153No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG12420.12314806919295378No Hit
CCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTG12130.12027263118442265No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGG12050.11947940690620718No Hit
CTTTTACTTCCAATGTTGTTTCCCCCACAGGTAATCCTGGCCGTCTTTCCTGGGGCCACTGACACCGAGGGTGG11970.11868618262799169No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11930.11828957048888396No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11730.11630650979334524No Hit
GTGTCAGTGGCCCCAGGAAAGACGGCCAGGATTACCTGTGGGGGAAACAA11720.11620735675856832No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC11660.1156124385499067No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11580.11481921427169123No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11550.11452175516736042No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT11400.11303445964570638No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11330.11234038840226782No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGCTCAGCTTGGTCCCTCCGCCGAATACCACTGAATGAT11140.11045648074150605No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC11110.11015902163717525No Hit
CAGTAATAGTCGGCCTCATCCCCGCCTTCGACCCTGCTGATGGTCAGGGT11050.10956410342851364No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11020.10926664432418284No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA10930.1083742670111904No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC10920.10827511397641348No Hit
GAATACCACTGAATGATCACTACTACCATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGCCTTCGACC10920.10827511397641348No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10770.10678781845475945No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10660.10569713507221315No Hit
GCTTGGTCCCTCCGCCGAATACCACTGAATGATCACTACTACCATCCCACACCTGACAGTAATAGTCGGCCTCAT10600.10510221686355155No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10400.10311915616801283No Hit
TCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACC10380.10292085009845897No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG10260.10173101368113574No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)