FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873362
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTG46800.5358602732887394No Hit
GTAATAGTCAGCCTCATCCTCTGACTGGAGGCCAGAGATGAGTAAAATCC39230.4491837290837018No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAATACCAC38800.4442602265727156No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC36650.41964271401778414No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35050.4013227046745794No Hit
GACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAATACCACAGCGTTGTTGTGCCAAATCATACAGTAATAGTCAG30860.3533471802070619No Hit
GTAATAGACAGCCGTGTCCTCGTGTCTCAGGCTGTTCATTTGCAGAAACA30570.350026678513606No Hit
GACTATTACTGTATGATTTGGCACAACAACGCTGTGGTATTCGGCGGAGGGACTAAGCTGACCGTCCTAGGTCAG28570.3271266668346001No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTAGTCCCTC25910.2966696513015221No Hit
GGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCC25900.29655515124312715No Hit
GTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATC25200.28854014715547505No Hit
GTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAG24960.2857921457539943No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24340.2786931421335025No Hit
GCTTAGTCCCTCCGCCGAATACCACAGCGTTGTTGTGCCAAATCATACAGTAATAGTCAGCCTCATCCTCTGA23450.26850263693634485No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23210.2657546355348641No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22510.257739631447212No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22480.25739613127202693No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT21780.24938112718437488No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21610.24743462619165935No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20450.2341526194178359No Hit
GGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGTCTCAC20240.23174811819154026No Hit
CACCTGCACCCTACGCAGTGGCATCAATGTTGGTACGTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTC20160.23083211772438003No Hit
CAGATAAGCAGTGGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAA19880.22762611608931918No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG19230.22018361229364228No Hit
GGACTAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG19190.21972561206006214No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18990.21743561089216154No Hit
CCCCAGTATCTCCTGAGGTTCAAATCAGACTCAGATAAGCAGTGGGGCTC18810.215374609841051No Hit
GTACGGGACTGTGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA18680.2138861090819156No Hit
CAGTAATAGTCAGCCTCATCCTCTGACTGGAGGCCAGAGATGAGTAAAAT18410.21079460750524984No Hit
CTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAG18260.20907710662932438No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18260.20907710662932438No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG18220.20861910639574424No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18180.20816110616216416No Hit
ATCCTGTACGTACCAACATTGATGCCACTGCGTAGGGTGCAGGTGAGACT18000.20610010511105362No Hit
GGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCT17550.20094760248327725No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC17540.20083310242488225No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17030.19499359944673572No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16990.19453559921315558No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16940.19396309892118044No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16890.19339059862920532No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAATAC16680.19098609740290967No Hit
GTCTATTACTGTGCGAGAGGTGGTGTAGTAGACTTTGACTACTGGGGCCA16450.188352596059824No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT16280.18640609506710848No Hit
GATAAGCAGTGGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTA16170.18514659442476317No Hit
GTATATCCTGTACGTACCAACATTGATGCCACTGCGTAGGGTGCAGGTGA15760.18045209203056695No Hit
GCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTACTCATCTCTGGC15680.1795360915634067No Hit
CTCCAGAGCCCCACTGCTTATCTGAGTCTGATTTGAACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTG15610.1787345911546415No Hit
CCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCT15570.17827659092106138No Hit
ATGTTGGTACGTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTGAGGTTCAAAT15400.17633008992834587No Hit
CTATTACTGTATGATTTGGCACAACAACGCTGTGGTATTCGGCGGAGGGACTAAGCTGACCGTCCTAGGTCAGCC15060.17243708794291485No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14910.17071958706698942No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14460.16556708443921306No Hit
TCTCTGGCCTCCAGTCAGAGGATGAGGCTGACTATTACTGTATGATTTGG14220.16281908303773235No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14160.16213208268736218No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC13990.16018558169464667No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13980.16007108163625164No Hit
GCTTATCTGAGTCTGATTTGAACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATATC13800.1580100805851411No Hit
GCCTCATCCTCTGACTGGAGGCCAGAGATGAGTAAAATCCCTGCATTGGCCGAAGCATCTTTGGATCCAGAGAAG13620.15594907953403056No Hit
ACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAATACCACAGCGTTGTTGTGCCAAATCATACAGTAATAGTCAGC13490.15446057877489516No Hit
CTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAG13340.15274307789896974No Hit
ATACTGGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATATCCTGTACG13240.15159807731501942No Hit
AGTATATCCTGTACGTACCAACATTGATGCCACTGCGTAGGGTGCAGGTGAGACTGGCTGATGCTCCAGGAGATG13020.14907907603032877No Hit
GGATGAGGCTGACTATTACTGTATGATTTGGCACAACAACGCTGTGGTATTCGGCGGAGGGACTAAGCTGACCGT12930.1480485755047735No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12840.14701807497921823No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12810.14667457480403315No Hit
GTGGTATTCGGCGGAGGGACTAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCA12670.14507157398650272No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTAGTC12560.14381207334415738No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12430.142323572585022No Hit
ACCATATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACTCACTGTTTCTG12340.14129307205946676No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC12340.14129307205946676No Hit
GCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGT12000.13740007007403574No Hit
GTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGG11600.13282006773823454No Hit
CTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCT11510.13178956721267926No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11460.13121706692070412No Hit
GAATACCACAGCGTTGTTGTGCCAAATCATACAGTAATAGTCAGCCTCAT11270.12904156581119855No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT11260.12892706575280355No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11230.12858356557761844No Hit
CAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCC11180.1280110652856433No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11170.12789656522724827No Hit
ATGCAGGGATTTTACTCATCTCTGGCCTCCAGTCAGAGGATGAGGCTGAC11100.12709506481848307No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10920.12503406376737253No Hit
GTATCTCCTGAGGTTCAAATCAGACTCAGATAAGCAGTGGGGCTCTGGAG10870.12446156347539737No Hit
GTATATGGTACTACTAGCAGTAGTAATGAATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTG10690.12240056242428683No Hit
CCTGCACCCTACGCAGTGGCATCAATGTTGGTACGTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTC10660.12205706224910175No Hit
GTCTCACCTGCACCCTACGCAGTGGCATCAATGTTGGTACGTACAGGATA10630.12171356207391665No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC10620.12159906201552163No Hit
GGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCT10610.1214845619571266No Hit
CTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCT10610.1214845619571266No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10320.11816406026367074No Hit
GTGCAGGTGAGACTGGCTGATGCTCCAGGAGATGCAGAGAGGGAAGACGG10310.1180495602052757No Hit
CTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCT10200.11679005956293038No Hit
GTATCAACGCAGAGTACGGGACTGTGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTC10160.11633205932935027No Hit
GTCCTCCCCAGTATCTCCTGAGGTTCAAATCAGACTCAGATAAGCAGTGGGGCTCTGGAGTCCCCAGCCGCTTCT10140.1161030592125602No Hit
CTGCTTATCTGAGTCTGATTTGAACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATAT10040.1149580586286099No Hit
CTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCC10000.11450005839502977No Hit
CTCCTGGAGCATCAGCCAGTCTCACCTGCACCCTACGCAGTGGCATCAAT9950.11392755810305463No Hit
GAGTACGGGACTGTGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA9900.11335505781107948No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG9890.11324055775268445No Hit
GCGTTGTTGTGCCAAATCATACAGTAATAGTCAGCCTCATCCTCTGACTGGAGGCCAGAGATGAGTAAAATCC9650.11049255635120375No Hit
GTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTG9370.10728655471614292No Hit
GAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTC9290.10637055424898266No Hit
GATTTTACTCATCTCTGGCCTCCAGTCAGAGGATGAGGCTGACTATTACT9100.1041950531394771No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC9090.10408055308108208No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC9060.10373705290589698No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9050.10362255284750196No Hit
GGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTT9020.10327905267231685No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC8950.10247755226355165No Hit
CTCCTGAGGTTCAAATCAGACTCAGATAAGCAGTGGGGCTCTGGAGTCCC8830.10110355156281128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3050.077.26626670
CGGGTAT150.00247522168.1415917
GTCGTAT453.0540832E-853.60671239
TTATACA3050.052.5025372
CTTATAC3050.051.4090081
CTAGCGG200.007568579651.38272534
CGTATGC2850.050.90617841
CACGAGA3150.049.76272621
TATACAC3300.049.5575183
TACACAT3400.049.104845
ATCTCGT2500.048.10674737
TCTCGTA2450.047.73008738
GTATGCC3150.047.22037542
TCGTATG2850.047.21794540
ACGAGAC3400.047.10595722
ATGCCGT2950.047.0160144
GGTATCA7150.046.24311
TCTCCGA3250.046.12925711
AGACACT3650.044.83109325
CCCACGA3500.044.78132219
Produced by FastQC (version 0.11.5)