FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873362
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTG45270.5183417643542998No Hit
GTAATAGTCAGCCTCATCCTCTGACTGGAGGCCAGAGATGAGTAAAATCC36910.42261971553605493No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC36150.41391771109803266No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAATACCAC35880.41082620952136684No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34580.395941201930013No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34350.3933077005869273No Hit
GACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAATACCACAGCGTTGTTGTGCCAAATCATACAGTAATAGTCAG30130.3449886759442247No Hit
GACTATTACTGTATGATTTGGCACAACAACGCTGTGGTATTCGGCGGAGGGACTAAGCTGACCGTCCTAGGTCAG27550.31544766087830706No Hit
GTAATAGACAGCCGTGTCCTCGTGTCTCAGGCTGTTCATTTGCAGAAACA27510.31498966064472694No Hit
GTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATC25980.2974711517102874No Hit
GTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAG25680.29403614995843647No Hit
GGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCC25400.2908301483233756No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23200.2656401354764691No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTAGTCCCTC23120.26472413500930886No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22880.2619761336078281No Hit
GCTTAGTCCCTCCGCCGAATACCACAGCGTTGTTGTGCCAAATCATACAGTAATAGTCAGCCTCATCCTCTGA22880.2619761336078281No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22300.2553351302209164No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT21300.24388512438141344No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21280.24365612426462338No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20420.2338091192426508No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20140.23060311760759No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG19810.226824615680554No Hit
GGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGTCTCAC19690.22545061497981364No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19320.2212141128191975No Hit
CACCTGCACCCTACGCAGTGGCATCAATGTTGGTACGTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTC19240.22029811235203728No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19120.21892411165129697No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAATAC19120.21892411165129697No Hit
GTACGGGACTGTGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA18370.2103366072716697No Hit
GGACTAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG17950.20552760481907847No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17830.2041536041183381No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17580.20129110265846234No Hit
CAGATAAGCAGTGGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAA17560.2010621025416723No Hit
ATCCTGTACGTACCAACATTGATGCCACTGCGTAGGGTGCAGGTGAGACT17460.19991710195772203No Hit
CAGTAATAGTCAGCCTCATCCTCTGACTGGAGGCCAGAGATGAGTAAAAT17410.19934460166574686No Hit
GGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCT17290.1979706009650065No Hit
CCCCAGTATCTCCTGAGGTTCAAATCAGACTCAGATAAGCAGTGGGGCTC17150.19636760014747606No Hit
CTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAG16930.19384859886278544No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16680.19098609740290967No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16380.1875510956510588No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16330.18697859535908365No Hit
GTCTATTACTGTGCGAGAGGTGGTGTAGTAGACTTTGACTACTGGGGCCA16150.1849175943079731No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC15920.18228409296488743No Hit
CCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCT15540.1779330907458763No Hit
ATGTTGGTACGTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTGAGGTTCAAAT15300.17518508934439556No Hit
GTATATCCTGTACGTACCAACATTGATGCCACTGCGTAGGGTGCAGGTGA15270.17484158916921047No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15220.17426908887723533No Hit
GATAAGCAGTGGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTA14610.1672845853151385No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14340.16419308373847272No Hit
GCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTACTCATCTCTGGC14130.16178858251217706No Hit
CTATTACTGTATGATTTGGCACAACAACGCTGTGGTATTCGGCGGAGGGACTAAGCTGACCGTCCTAGGTCAGCC14040.1607580819866218No Hit
CTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAG13450.15400257854131505No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13360.1529720780157598No Hit
GTGGTATTCGGCGGAGGGACTAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCA13280.15205607754859957No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTAGTC13260.15182707743180948No Hit
TCTCTGGCCTCCAGTCAGAGGATGAGGCTGACTATTACTGTATGATTTGG13220.15136907719822937No Hit
GCTTATCTGAGTCTGATTTGAACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATATC13210.15125457713983434No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC13020.14907907603032877No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC12880.14747607521279837No Hit
ACCATATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACTCACTGTTTCTG12830.1469035749208232No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12730.14575857433687292No Hit
GGATGAGGCTGACTATTACTGTATGATTTGGCACAACAACGCTGTGGTATTCGGCGGAGGGACTAAGCTGACCGT12690.1453005741032928No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12570.14392657340255244No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12450.14255257270181207No Hit
AGTATATCCTGTACGTACCAACATTGATGCCACTGCGTAGGGTGCAGGTGAGACTGGCTGATGCTCCAGGAGATG12410.14209457246823196No Hit
ATACTGGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATATCCTGTACG12280.1406060717090966No Hit
GCCTCATCCTCTGACTGGAGGCCAGAGATGAGTAAAATCCCTGCATTGGCCGAAGCATCTTTGGATCCAGAGAAG12160.13923207100835622No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC11960.13694206984045562No Hit
ACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAATACCACAGCGTTGTTGTGCCAAATCATACAGTAATAGTCAGC11900.13625506949008545No Hit
GCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGT11870.13591156931490034No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11860.13579706925650534No Hit
GAATACCACAGCGTTGTTGTGCCAAATCATACAGTAATAGTCAGCCTCAT11620.1330490678550246No Hit
GTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGG11610.13293456779662957No Hit
CTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCT11460.13121706692070412No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11310.1294995660447787No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11280.12915606586959358No Hit
ATGCAGGGATTTTACTCATCTCTGGCCTCCAGTCAGAGGATGAGGCTGAC11100.12709506481848307No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11070.12675156464329798No Hit
GAGTACGGGACTGTGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA10570.12102656172354649No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10540.12068306154836138No Hit
GTATCAACGCAGAGTACGGGACTGTGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTC10470.11988156113959618No Hit
GTCTCACCTGCACCCTACGCAGTGGCATCAATGTTGGTACGTACAGGATA10370.11873656055564588No Hit
CTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCC10370.11873656055564588No Hit
CTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCT10240.1172480597965105No Hit
GGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTT10240.1172480597965105No Hit
CTGCTTATCTGAGTCTGATTTGAACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATAT10180.11656105944614031No Hit
GTATATGGTACTACTAGCAGTAGTAATGAATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTG10140.1161030592125602No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT10110.11575955903737511No Hit
CTCCTGGAGCATCAGCCAGTCTCACCTGCACCCTACGCAGTGGCATCAAT10030.11484355857021486No Hit
GGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCT10000.11450005839502977No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9970.1141565582198447No Hit
GTATCTCCTGAGGTTCAAATCAGACTCAGATAAGCAGTGGGGCTCTGGAG9960.11404205816144966No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG9950.11392755810305463No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9940.11381305804465959No Hit
CAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCC9910.11346955786947452No Hit
GTCCTCCCCAGTATCTCCTGAGGTTCAAATCAGACTCAGATAAGCAGTGGGGCTCTGGAGTCCCCAGCCGCTTCT9890.11324055775268445No Hit
CCTGCACCCTACGCAGTGGCATCAATGTTGGTACGTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTC9870.11301155763589439No Hit
GCGTTGTTGTGCCAAATCATACAGTAATAGTCAGCCTCATCCTCTGACTGGAGGCCAGAGATGAGTAAAATCC9820.11243905734391925No Hit
GTGCAGGTGAGACTGGCTGATGCTCCAGGAGATGCAGAGAGGGAAGACGG9680.11083605652638882No Hit
CTCCAGAGCCCCACTGCTTATCTGAGTCTGATTTGAACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTG9660.11060705640959875No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC9400.10763005489132799No Hit
CTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCT9380.10740105477453793No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC9270.10614155413219262No Hit
GTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTG9260.10602705407379757No Hit
CTCCTGAGGTTCAAATCAGACTCAGATAAGCAGTGGGGCTCTGGAGTCCC9130.10453855331466219No Hit
GAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTC8980.10282105243873674No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC8900.10190505197157651No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC8830.10110355156281128No Hit
GATTTTACTCATCTCTGGCCTCCAGTCAGAGGATGAGGCTGACTATTACT8830.10110355156281128No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAG150.00248114468.10052518
AAGCGTG400.0074756651.53912470
GGTATCA7500.044.4770471
TGGTATC2350.041.9809762
CAGGTAT651.281478E-841.893611
AGGTATC603.248224E-739.688912
GTATCAA18550.036.8827481
TTAACGG601.4011299E-534.28407335
CACATGT601.4494417E-534.1147828
GCATTGT7150.032.0779562
TATTAGA851.366243E-732.017952
GTGAGTT7050.031.84529951
TTTGGAT16750.031.5901570
ACGGGCT652.5406318E-531.42740616
TATCAAC21800.031.2101522
ATTGTCT7400.031.14074364
TACTGTG5150.031.0554187
TTGGCAT7450.031.0332759
AGTGAGT7250.030.90112950
TGTGCGA5100.030.69265210
Produced by FastQC (version 0.11.5)