FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352469
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCTGCGGCGGTCACAGACCTCAGGTTCAGGGAGA11390.3231489861519736No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9900.2808757649608902No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9020.25590903029769996No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8290.23519798904300804No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT7970.22611917643821158No Hit
CAGTAGTATGTCGCAAAATCTTCGGGCTGCAGATTGCTGATGGTGAGAGTGAACTCTGTGCCAGATTTACTGCCA6830.19377590653362423No Hit
GAGTTCACTCTCACCATCAGCAATCTGCAGCCCGAAGATTTTGCGACATA6760.19178991627632502No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6660.18895278733732612No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6500.1844133810349279No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6330.17959026183862978No Hit
GAGTGAACTCTGTGCCAGATTTACTGCCACTGAACCTCGATGGGACTCCA6210.1761857071118311No Hit
GTAGTATGTCGCAAAATCTTCGGGCTGCAGATTGCTGATGGTGAGAGTGAACTCTGTGCCAGATTTACTGCCA6140.1741997168545319No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6010.17051144923383332No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGAGCAAAGGAACTGTGGCTGCACCA5950.168809171870434No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5820.16512090424973544No Hit
ATACTACTGTCTGCAGACTTCCACTACTTTGTACACTTTTGGCCAGGGGA5540.15717694322053855No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT5420.15377238849373986No Hit
GTATATTACTGTGCGAGACGGGGACACGTAGGCTTCAATTACTACTTCGA5410.15348867559983997No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG5400.15320496270594008No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC5300.1503678337669412No Hit
ACATACTACTGTCTGCAGACTTCCACTACTTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGAGCAAAGGAA5250.14894926929744176No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4980.14128902116214476No Hit
GATCAGGAGCGCAGGGGCTCTCCCTGGTCTCTGTTGATACCAATGCAAAT4950.1404378824804451No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT4890.13873560511704575No Hit
GTAGTGGAAGTCTGCAGACAGTAGTATGTCGCAAAATCTTCGGGCTGCAGATTGCTGATGGTGAGAGTGAACT4840.1373170406475463No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT4690.13306134723904797No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG4490.1273870893610502No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA4410.12511738620985108No Hit
GATCTATGCTGCATCTAATTTACAAAGTGGAGTCCCATCGAGGTTCAGTGGCAGTAAATCTGGCACAGAGTTCA4380.12426624752815141No Hit
GCATAGATCAGGAGCGCAGGGGCTCTCCCTGGTCTCTGTTGATACCAATG4370.12398253463425152No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC4320.12256397016475208No Hit
GGTATCAACAGAGACCAGGGAGAGCCCCTGCGCTCCTGATCTATGCTGCATCTAATTTACAAAGTGGAGTCCCAT4300.1219965443769523No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT4280.12142911858915252No Hit
CTTCCACTACTTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGAGCAAA4210.1194431283318533No Hit
GACCAGGGAGAGCCCCTGCGCTCCTGATCTATGCTGCATCTAATTTACAA4210.1194431283318533No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA4170.11830827675625374No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG4060.11518743492335497No Hit
CTCCTGATCTATGCTGCATCTAATTTACAAAGTGGAGTCCCATCGAGGTT4030.11433629624165528No Hit
GTGGAGTCCCATCGAGGTTCAGTGGCAGTAAATCTGGCACAGAGTTCACT4010.1137688704538555No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT3860.1095131770453572No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA3760.1066760481063583No Hit
CTCCCTGGTCTCTGTTGATACCAATGCAAATAGTCGCTGATGGTCTGACTTGCCCGGCAAGTGATGGTGACT3700.10497377074295895No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAAGATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC3690.10469005784905906No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA3680.10440634495515917No Hit
GTTGATACCAATGCAAATAGTCGCTGATGGTCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATG3680.10440634495515917No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG3680.10440634495515917No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC3670.10412263206125928No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT3630.10298778048565974No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATCAGCGACTATTTGCATTGGTATCAACAGAGA3620.10270406759175985No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT3530.10015065154666085No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT3530.10015065154666085No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGTA150.00297118165.0652711
AATACGT150.00297118165.0652710
TGAGTTC200.006805498652.7750545
CCGAGTT303.665138E-451.34699259
ACGGGTA200.00927591448.79895416
GGTAGAC200.00927591448.7989542
TACGTAC200.00928111748.79202712
GACTGAT3800.046.56422470
ATACTGG308.4142544E-443.3836
TTACTTT709.703758E-442.1295470
GTAAGAT350.001091126741.150449
AACAGGC350.001451040338.83309639
CGAGTTC400.001469564338.73142260
TTGCCGA400.0016484637.83663656
CGTATGC557.531642E-637.52393341
GTACCTA350.001793079837.1959881
TAATACT451.4129897E-436.1473734
TATACAC551.10380915E-535.4901433
CTCTAAT658.5806823E-735.050061
GTATGCC601.3067689E-534.62549242
Produced by FastQC (version 0.11.5)