FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352469
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCTGCGGCGGTCACAGACCTCAGGTTCAGGGAGA11200.31775844116787577No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9870.2800246262791905No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9250.26243442685739743No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8030.22782145380161092No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT7900.22413318618091238No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7890.22384947328701246No Hit
GAGTTCACTCTCACCATCAGCAATCTGCAGCCCGAAGATTTTGCGACATA7000.19859902572992236No Hit
CAGTAGTATGTCGCAAAATCTTCGGGCTGCAGATTGCTGATGGTGAGAGTGAACTCTGTGCCAGATTTACTGCCA6860.1946270452153239No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6320.1793065489447299No Hit
GAGTGAACTCTGTGCCAGATTTACTGCCACTGAACCTCGATGGGACTCCA6270.17788798447523044No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6230.17675313289963088No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6140.1741997168545319No Hit
GTAGTATGTCGCAAAATCTTCGGGCTGCAGATTGCTGATGGTGAGAGTGAACTCTGTGCCAGATTTACTGCCA5960.1690928847643339No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5920.16795803318873434No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG5600.15887922058393789No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC5400.15320496270594008No Hit
ATACTACTGTCTGCAGACTTCCACTACTTTGTACACTTTTGGCCAGGGGA5310.1506515466608411No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT5310.1506515466608411No Hit
ACATACTACTGTCTGCAGACTTCCACTACTTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGAGCAAAGGAA5210.1478144177218422No Hit
GTATATTACTGTGCGAGACGGGGACACGTAGGCTTCAATTACTACTTCGA5060.14355872431334385No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT4930.1398704566926453No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC4920.13958674379874544No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT4810.13646590196584663No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGAGCAAAGGAACTGTGGCTGCACCA4660.1322102085573483No Hit
GGTATCAACAGAGACCAGGGAGAGCCCCTGCGCTCCTGATCTATGCTGCATCTAATTTACAAAGTGGAGTCCCAT4510.12795451514884998No Hit
CTCCTGATCTATGCTGCATCTAATTTACAAAGTGGAGTCCCATCGAGGTT4490.1273870893610502No Hit
GATCTATGCTGCATCTAATTTACAAAGTGGAGTCCCATCGAGGTTCAGTGGCAGTAAATCTGGCACAGAGTTCA4490.1273870893610502No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG4480.1271033764671503No Hit
GATCAGGAGCGCAGGGGCTCTCCCTGGTCTCTGTTGATACCAATGCAAAT4430.12568481199765086No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4260.12086169280135274No Hit
GCATAGATCAGGAGCGCAGGGGCTCTCCCTGGTCTCTGTTGATACCAATG4240.12029426701355296No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA4230.12001055411965307No Hit
GTAGTGGAAGTCTGCAGACAGTAGTATGTCGCAAAATCTTCGGGCTGCAGATTGCTGATGGTGAGAGTGAACT4220.1197268412257532No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT4200.11915941543795341No Hit
GACCAGGGAGAGCCCCTGCGCTCCTGATCTATGCTGCATCTAATTTACAA4190.11887570254405352No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG4150.11774085096845396No Hit
CTCCCTGGTCTCTGTTGATACCAATGCAAATAGTCGCTGATGGTCTGACTTGCCCGGCAAGTGATGGTGACT4080.11575486071115475No Hit
CTTCCACTACTTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGAGCAAA4020.11405258334775539No Hit
GTGGAGTCCCATCGAGGTTCAGTGGCAGTAAATCTGGCACAGAGTTCACT3870.10979688993925708No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA3840.1089457512575574No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATCAGCGA3780.10724347389415806No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT3750.1063923352124584No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT3660.10383891916735939No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG3640.10327149337955963No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA3630.10298778048565974No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC3590.10185292891006018No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGCG150.001594150876.12269657
GACTGAT4500.061.2756370
GACCTTA200.00665360253.0770546
CACTCGC250.007137588752.14021768
GTTTCAA253.4533773E-451.9725342
TTTAAGT604.3055136E-449.68294570
CTACGCA200.009068249.07939527
ATTTAAG404.954542E-448.2993769
ACTCGCC300.009656914548.29936669
GTGGTAT900.046.9796371
CCGCTAT656.388753E-445.8611870
CGACTGA4600.040.94946369
AGTTTAT806.548362E-1140.6035422
GATTCAC350.001799074937.1707041
AATTCAT350.0018078837.1337748
ATTCACT350.00181040237.1232382
GGTTATC350.00181040237.1232382
TCGACTG4650.036.4420968
TGACTAA450.00231950535.2933363
GCTATCC651.2247128E-534.9534156
Produced by FastQC (version 0.11.5)